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Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of virus-free plants

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Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of virus-free plants

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dc.contributor.author San Pedro-Galan, Tania es_ES
dc.contributor.author Gammoudi, Najet es_ES
dc.contributor.author Peiró Barber, Rosa Mª es_ES
dc.contributor.author Olmos Castelló, Antonio es_ES
dc.contributor.author Gisbert Domenech, María Carmen es_ES
dc.date.accessioned 2018-06-09T04:21:31Z
dc.date.available 2018-06-09T04:21:31Z
dc.date.issued 2017 es_ES
dc.identifier.issn 1471-2229 es_ES
dc.identifier.uri http://hdl.handle.net/10251/103709
dc.description.abstract [EN] Background: Somatic embryogenesis is the preferred method for cell to plant regeneration in Vitis vinifera L. However, low frequencies of plant embryo conversion are commonly found. In a previous work we obtained from cut-seeds of a grapevine infected with the Grapevine leafroll associated viruses 1 and 3 (GLRaV-1 and GLRaV-3), high rates of direct regeneration, embryo plant conversion and sanitation. The aim of this study is to evaluate the usefulness of this procedure for regeneration of other grapevine varieties which include some infected with one to three common grapevine viruses (GLRaV-3, Grapevine fanleaf virus (GFLV) and Grapevine fleck virus (GFkV)). As grapevine is highly heterozygous, it was necessary to select from among the virus-free plants those that regenerated from mother tissues around the embryo, (true-to-type). Results: Somatic embryogenesis and plant regeneration were achieved in a first experiment, using cut-seeds from the 14 grapevine varieties Airen, Cabernet Franc, Cabernet Sauvignon, Mencia, Merlot, Monastrell, Petit Verdot, Pinot Blanc (infected by GFLV and GFkV), Pinot Gris, Pinot Meunier, Pinot Noir, Syrah, Tempranillo (infected by GFLV), and Verdil. All regenerated plants were confirmed to be free of GFkV whereas at least 68% sanitation was obtained for GFLV. The SSR profiles of the virus-free plants showed, in both varieties, around 10% regeneration from mother tissue (the same genetic make-up as the mother plant). In a second experiment, this procedure was used to sanitize the varieties Cabernet Franc, Godello, Merlot and Valenci Blanc infected by GLRaV-3, GFkV and/or GFLV. Conclusions: Cut-seeds can be used as explants for embryogenesis induction and plant conversion in a broad range of grapevine varieties. The high regeneration rates obtained with this procedure facilitate the posterior selection of true-to-type virus-free plants. A sanitation rate of 100% was obtained for GFkV as this virus is not seed-transmitted. However, the presence of GLRaV-3 and GFLV in some of the regenerated plants showed that both viruses are seed-transmitted. The regeneration of true-to-type virus-free plants from all infected varieties indicates that this methodology may represent an alternative procedure for virus cleaning in grapevine. es_ES
dc.description.sponsorship The study was supported by the INIA (Instituto Nacional de Investigaciones Agrarias) projects RTA2011-00067-C04, RTA2014-00061-C03 and the MINECO (Ministerio de Economia y Competitividad) project CGL2015-70843-R, all co-funded with FEDER Funds. TSP has a fellowship (01/14-FSE-22) from the Instituto Valenciano de Investigaciones Agrarias (IVIA). en_EN
dc.language Inglés es_ES
dc.publisher Springer (Biomed Central Ltd.) es_ES
dc.relation.ispartof BMC Plant Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Grapevine es_ES
dc.subject Direct and indirect embryogenesis es_ES
dc.subject Microsatellites es_ES
dc.subject TDZ es_ES
dc.subject Sanitation es_ES
dc.subject.classification GENETICA es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of virus-free plants es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12870-017-1159-3 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CGL2015-70843-R/ES/DESARROLLO DE PROTOCOLOS DE CONSERVACION IN VITRO Y DE CRIOCONSERVACION DE GERMOPLASMA DE VID: ANALISIS DE LA VARIABILIDAD Y CONSERVACION DE PORTAINJERTOS Y VARIEDADES MINORIT/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//RTA2011-00067-C04-04/ES/Desarrollo y aplicación de distintas técnicas de cultivo in vitro para el saneamiento, micropropagación y conservación de variedades de vid. Desarrollo de un sistema de purificación de protoplastos de vid adecuado para el estudio de la infección y de la replicación vírica/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation San Pedro-Galan, T.; Gammoudi, N.; Peiró Barber, RM.; Olmos Castelló, A.; Gisbert Domenech, MC. (2017). Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of virus-free plants. BMC Plant Biology. 17(226):1-12. https://doi.org/10.1186/s12870-017-1159-3 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1186/s12870-017-1159-3 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 12 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 17 es_ES
dc.description.issue 226 es_ES
dc.identifier.pmid 29187140 en_EN
dc.identifier.pmcid PMC5706158 en_EN
dc.relation.pasarela S\343302 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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