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Dynamics of calcium during in vitro microspore embryogenesis and in vivo microspore development in Brassica napus and Solanum melongena

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Dynamics of calcium during in vitro microspore embryogenesis and in vivo microspore development in Brassica napus and Solanum melongena

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dc.contributor.author Rivas-Sendra, Alba es_ES
dc.contributor.author Calabuig-Serna, Antonio es_ES
dc.contributor.author Seguí-Simarro, Jose M. es_ES
dc.date.accessioned 2018-06-18T04:22:21Z
dc.date.available 2018-06-18T04:22:21Z
dc.date.issued 2017 es_ES
dc.identifier.uri http://hdl.handle.net/10251/104244
dc.description.abstract [EN] Calcium is widely known to have a role as a signaling molecule in many different processes, including stress response and activation of the embryogenic program. However, there are no direct clues about calcium levels during microspore embryogenesis, an experimental process that combines a developmental switch toward embryogenesis and the simultaneous application of different stressing factors. In this work, we used FluoForte, a calcium-specific fluorescent vital dye, to track by confocal microscopy the changes in levels and subcellular distribution of calcium in living rapeseed (B. napus) and eggplant (S. melongena) microspores and pollen grains during in vivo development, as well as during the first stages of in vitro-induced microspore embryogenesis in rapeseed. During in vivo development, a clear peak of cytosolic Ca2+ was observed in rapeseed vacuolate microspores and young pollen grains, the stages more suitable for embryogenesis induction. However, the Ca2+ levels observed in eggplant were dramatically lower than in rapeseed. Just after in vitro induction, Ca2+ levels increased specifically in rapeseed embryogenic microspores at levels dramatically higher than during in vivo development. The increase was observed in the cytosol, but predominantly in vacuoles. Non-embryogenic forms such as callus-like and pollen-like structures presented remarkably different calcium patterns. After the heat shock-based inductive treatment, Ca2+ levels progressively decreased in all cases. Together, our results reveal unique calcium dynamics in in vivo rapeseed microspores, as well as in those reprogrammed to in vitro embryogenesis, establishing a link between changes in Ca2+ level and subcellular distribution, and microspore embryogenesis. es_ES
dc.description.sponsorship This work was supported by grant AGL2014-55177-R to JS from Spanish Ministerio de Economia y Competitividad (MINECO) jointly funded by FEDER. AR was supported by a predoctoral fellowship from the FPI Program of Universitat Politecnica de Valencia.
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//AGL2014-55177-R/ES/NUEVAS VIAS DE MEJORA DE LA EMBRIOGENESIS DE MICROSPORAS EN SOLANACEAS RECALCITRANTES: ESTUDIO DE LA AUTOFAGIA, LA UPR Y LA REGULACION HORMONAL/ es_ES
dc.relation.ispartof Frontiers in Plant Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Androgenesis es_ES
dc.subject Eggplant es_ES
dc.subject FluoForte es_ES
dc.subject In vitro culture es_ES
dc.subject Microgametogenesis es_ES
dc.subject Microspore culture es_ES
dc.subject Microsporogenesis es_ES
dc.subject Rapeseed es_ES
dc.subject.classification GENETICA es_ES
dc.title Dynamics of calcium during in vitro microspore embryogenesis and in vivo microspore development in Brassica napus and Solanum melongena es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fpls.2017.01177 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Rivas-Sendra, A.; Calabuig-Serna, A.; Seguí-Simarro, JM. (2017). Dynamics of calcium during in vitro microspore embryogenesis and in vivo microspore development in Brassica napus and Solanum melongena. Frontiers in Plant Science. 8. doi:10.3389/fpls.2017.01177 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.3389/fpls.2017.01177 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.identifier.eissn 1664-462X es_ES
dc.identifier.pmid 28736567
dc.identifier.pmcid PMC5500647
dc.relation.pasarela S\340795 es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES


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