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First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity

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First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity

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dc.contributor.author Asturiano Nemesio, Juan Francisco es_ES
dc.contributor.author Sorensen, S.R. es_ES
dc.contributor.author Pérez Igualada, Luz María es_ES
dc.contributor.author Lauesen, P. es_ES
dc.contributor.author Tomkiewicz, J. es_ES
dc.date.accessioned 2018-06-28T08:21:01Z
dc.date.available 2018-06-28T08:21:01Z
dc.date.issued 2016 es_ES
dc.identifier.issn 0936-6768 es_ES
dc.identifier.uri http://hdl.handle.net/10251/104774
dc.description.abstract [EN] Contents Sperm cryopreservation is a useful tool in captive fish reproduction management, that is to synchronize gamete production, especially in the case of species as the European eel, where the time of female spawning readiness is unpredictable. Several protocols to cryopreserve sperm of this species have been described, but until recently fertilization trials were not feasible. This study evaluated the effect of cold storage of diluted sperm prior to fertilizations and tested whether a previously defined protocol for European eel sperm cryopreservation can be successfully applied in fertilization trials to produce viable offspring. In our experiment, the sperm motility was evaluated after the extraction and the best samples were selected and pooled. Until stripping of eggs and fertilization, diluted sperm samples were maintained at either 4 or 20 degrees C, or cryopreserved, following existing protocols. Fertilization of two egg batches was attempted. Diluted sperm caused a similar percentage of fertilized eggs and a similar number of embryos and larvae, independently of storage temperature (4 or 20 degrees C). The cryopreserved sperm resulted in a lower percentage of fertilized eggs, but embryos developed and a few larvae (cryolarvae') were obtained 55h after fertilization in one of the two egg batches. This result evidences that the tested cryopreservation protocol is applicable for eel reproduction management, although improvements will be required to enhance fertilization success. es_ES
dc.description.sponsorship This study was part of the project: 'Reproduction of European Eel: Towards a Self-sustained Aquaculture' (PRO-EEL) European Community's 7th Framework Programme under the Theme 2 'Food, Agriculture and Fisheries, and Biotechnology', Grant Agreement no245257. Juan F. Asturiano and Luz Perez had a grant to staying in Denmark from Programa de Apoyo a la Investigacion y Desarrollo (PAID-00-11) of the Universitat Politecnica de Valencia. We thank Christian Graver and Lars B. Tybjerg for assistance during en_EN
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation COMISION DE LAS COMUNIDADES EUROPEA/245257 es_ES
dc.relation.ispartof Reproduction in Domestic Animals es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cryopreservation es_ES
dc.subject Spermatozoa es_ES
dc.subject Fertilization es_ES
dc.subject Eel larvae es_ES
dc.subject Anguilla anguilla es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/rda.12706 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Asturiano Nemesio, JF.; Sorensen, S.; Pérez Igualada, LM.; Lauesen, P.; Tomkiewicz, J. (2016). First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity. Reproduction in Domestic Animals. 51(4):485-491. doi:10.1111/rda.12706 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1111/rda.12706 es_ES
dc.description.upvformatpinicio 485 es_ES
dc.description.upvformatpfin 491 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 51 es_ES
dc.description.issue 4 es_ES
dc.relation.pasarela S\315797 es_ES
dc.contributor.funder European Commission es_ES


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