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Molecular and biological characterization of an isolate of Tomato mottle mosaic virus (ToMMV) infecting tomato and other experimental hosts in eastern Spain

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Molecular and biological characterization of an isolate of Tomato mottle mosaic virus (ToMMV) infecting tomato and other experimental hosts in eastern Spain

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dc.contributor.author Ambros Palaguerri, Silvia es_ES
dc.contributor.author Martinez, F. es_ES
dc.contributor.author Ivars, P. es_ES
dc.contributor.author Hernandez Fort, Carmen es_ES
dc.contributor.author De la Iglesia Jordán, Francisca es_ES
dc.contributor.author Elena Fito, Santiago Fco. es_ES
dc.date.accessioned 2018-06-28T08:22:20Z
dc.date.available 2018-06-28T08:22:20Z
dc.date.issued 2017 es_ES
dc.identifier.issn 0929-1873 es_ES
dc.identifier.uri http://hdl.handle.net/10251/104777
dc.description.abstract [EN] Tomato is known to be a natural and experimental reservoir host for many plant viruses. In the last few years a new tobamovirus species, Tomato mottle mosaic virus (ToMMV), has been described infecting tomato and pepper plants in several countries worldwide. Upon observation of symptoms in tomato plants growing in a greenhouse in Valencia, Spain, we aimed to ascertain the etiology of the disease. Using standard molecular techniques, we first detected a positive sense single-stranded RNA virus as the probable causal agent. Next, we amplified and sequenced its full-length genomic RNA which identified the virus as a new ToMMV isolate. Through extensive assays on distinct plant species, we investigated the host range of the Spanish ToMMV isolate. Several plant species were locally and/or systemically infected by the virus, some of which had not been previously reported as ToMMV hosts despite they are commonly used in research greenhouses. Finally, two reliable molecular diagnostic techniques were developed and used to assess the presence of ToMMV. This is the first observation of ToMMV in tomato plants in Europe. We discuss the possibility that, given the high sequence homology between ToMMV and Tomato mosaic virus, the former may have been mistakenly diagnosed as the latter by serological methods. es_ES
dc.description.sponsorship This work was supported by grants BFU2015-70261-P and BFU2015-65037-P (to C.H. and S.F.E., respectively) from Spain Ministry of Economy, Industry and Competitiveness/FEDER. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation Ministerio de Economía y Competitividad/BFU2015-70261-P es_ES
dc.relation Ministerio de Economía, Industria y Competitividad/BFU2015-65037-P es_ES
dc.relation.ispartof European Journal of Plant Pathology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Tomato mottle mosaic virus es_ES
dc.subject Emerging virus es_ES
dc.subject Diagnostic techniques es_ES
dc.subject Experimental host range es_ES
dc.subject Biological characterization es_ES
dc.subject Tobamovirus es_ES
dc.title Molecular and biological characterization of an isolate of Tomato mottle mosaic virus (ToMMV) infecting tomato and other experimental hosts in eastern Spain es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10658-017-1180-2 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Ambros Palaguerri, S.; Martinez, F.; Ivars, P.; Hernandez Fort, C.; De La Iglesia Jordán, F.; Elena Fito, SF. (2017). Molecular and biological characterization of an isolate of Tomato mottle mosaic virus (ToMMV) infecting tomato and other experimental hosts in eastern Spain. European Journal of Plant Pathology. 149(2):261-268. doi:10.1007/s10658-017-1180-2 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s10658-017-1180-2 es_ES
dc.description.upvformatpinicio 261 es_ES
dc.description.upvformatpfin 268 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 149 es_ES
dc.description.issue 2 es_ES
dc.relation.pasarela S\356338 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES
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