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Subjective and objective assessment of fish sperm motility: when the technique and technicians matter

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Subjective and objective assessment of fish sperm motility: when the technique and technicians matter

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dc.contributor.author Gallego Albiach, Victor es_ES
dc.contributor.author Herranz-Jusdado, Juan Germán es_ES
dc.contributor.author Rozenfeld, Christoffer es_ES
dc.contributor.author Pérez Igualada, Luz María es_ES
dc.contributor.author Asturiano Nemesio, Juan Francisco es_ES
dc.date.accessioned 2018-07-02T04:22:03Z
dc.date.available 2018-07-02T04:22:03Z
dc.date.issued 2018 es_ES
dc.identifier.issn 0920-1742 es_ES
dc.identifier.uri http://hdl.handle.net/10251/104902
dc.description.abstract [EN] Fish sperm motility is nowadays considered the best sperm quality biomarker in fish, and can be evaluated both by subjective and computerized methods. With the aim to compare the precision and accuracy of both techniques, fish sperm samples were assessed by subjective methods and by a computerassisted sperm analysis (CASA-Mot) system, and simultaneously by three different technicians with different degrees of expertise on the sperm quality analysis. Statistical dispersion parameters (CV, coefficient of variation; and RG, range) were estimated in order to determine the precision and accuracy of the techniques and the influence of laboratory staff on sperm motion assessments. Concerning precision, there were not much significant differences between the technical support staff (high, medium, and low experimented technician), and statistical dispersion parameters were quite similar between them independent of the technique used and the sperm motility class analyzed. However, concerning accuracy, experimented technician reported subjective motility values very closed to the values provided by the CASA-Mot system, only 10 percentage points away from the data provided by a CASA-Mot system. However, medium and low experimented technicians often overestimate the CASA-Mot values, and amplitudes up to 30 percentage points were detected in several sperm assessments. To sum up, both the technique (subjective or objective) and the technician (degree of expertise) became key factors in order to reach accurate motility estimations, so the use of both qualified staff and novel CASA-Mot systems seems to be a critical requirement for obtaining satisfying results in fish species with similar motility patterns.
dc.description.sponsorship This study is funded by the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 642893 (IMPRESS) and the COST Office (COST Action FA1205: AQUAGAMETE). VG has a postdoc grant from the UPV (PAID-10-16).
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Fish Physiology and Biochemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Subjective and objective assessment of fish sperm motility: when the technique and technicians matter es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10695-018-0505-1 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/642893/EU/Improved production strategies for endangered freshwater species./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-10-16/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/COST//FA1205/EU/Assessing and improving the quality of aquatic animal gametes to enhance aquatic resources - The need to harmonize and standardize evolving methodologies, and improve transfer from academia to industry/ es_ES
dc.rights.accessRights Abierto es_ES
dc.date.embargoEndDate 2019-04-30 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Gallego Albiach, V.; Herranz-Jusdado, JG.; Rozenfeld, C.; Pérez Igualada, LM.; Asturiano Nemesio, JF. (2018). Subjective and objective assessment of fish sperm motility: when the technique and technicians matter. Fish Physiology and Biochemistry. 1-11. https://doi.org/10.1007/s10695-018-0505-1 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s10695-018-0505-1 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 11 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.relation.pasarela S\364185 es_ES
dc.contributor.funder European Commission
dc.contributor.funder European Cooperation in Science and Technology es_ES
dc.contributor.funder Universitat Politècnica de València
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