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Testing cryopreserved European eel sperm for hybridization (A. japonica × A. anguilla)

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Testing cryopreserved European eel sperm for hybridization (A. japonica × A. anguilla)

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dc.contributor.author Müller, T. es_ES
dc.contributor.author Matsubara, H. es_ES
dc.contributor.author Kubara, Y. es_ES
dc.contributor.author Horváth, Ákos es_ES
dc.contributor.author Kolics, B. es_ES
dc.contributor.author Taller, J. es_ES
dc.contributor.author Stéger, V. es_ES
dc.contributor.author Kovács, B. es_ES
dc.contributor.author Horváth, L. es_ES
dc.contributor.author Asturiano Nemesio, Juan Francisco es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Urbanyi, B. es_ES
dc.date.accessioned 2018-07-02T04:24:01Z
dc.date.available 2018-07-02T04:24:01Z
dc.date.issued 2018 es_ES
dc.identifier.issn 0093-691X es_ES
dc.identifier.uri http://hdl.handle.net/10251/104907
dc.description.abstract [EN] The objective of this study was to assess impact of cryopreserved European eel sperm and Japanese eel native sperm on early fertilization, hatch, survival, and malformation rates of larvae, as well as develop molecular techniques to distinguish different eel species. Eggs from Japanese eel females (Anguilla japonica) were artificially fertilized with sperm of Japanese eel males and cryopreserved sperm from European eel (A. anguilla, extender was modified Tanaka solution and methanol as cryoprotectant). There were no statistical differences (p¿>¿0.05) among the measured parameters such as fertilization, hatch and survival after 10 days post-hatch rates due to large individual differences. The malformation rate of larvae compared to the hatching rate was higher in cryopreserved groups than in the control indicating that the methodology needs further refinement. Genetic analyses (PCR-RFLP, PCR-HRM) proved a clear result in the detection of paternal contribution in hybridization between the Japanese and the European eel and applied PCR-HRM method is a quick and cost effective tool to identify illegally imported A. anguilla at the glass eel stage, which can be transported from Europe to Asia. es_ES
dc.description.sponsorship The research was supported by The Ministry of Education, Culture, Sports, Science and Technology (MEXT)/Japan Society for the Promotion of Science (JSPS) Kakenhi Grant No.15K07562 and Tokyo University of Agriculture Strategic Research Program (TUA-SRP), Mohamed bin Zayed Species Conservation Fund (grant number 12252178), GINOP-2.3.2-15-2016-00054 project of the National Research, Development and Innovation Office of Hungary and EFOP-3.6.3-VEKOP-16-2017-00008 project. The project is co-financed by the European Union, the European Social Fund and KMR_12-1-2012-0435. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Theriogenology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Propagation es_ES
dc.subject Eel larvae es_ES
dc.subject SNP es_ES
dc.subject High resolution melting analyses es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Testing cryopreserved European eel sperm for hybridization (A. japonica × A. anguilla) es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.theriogenology.2018.02.021 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NKFIH//GINOP-2.3.2-15-2016-00054/
dc.relation.projectID info:eu-repo/grantAgreement/NKFIH//KMR_12-1-2012-0435/HU/Biomanipuláció és biotechnikai módszerek alkalmazása az angolna ivarérlelésének és indukált szaporításának elősegítésének céljából (komplex felkészítési programok, inoxikáció, gaméta krioprezerváció, ivari hormonháztartás befolyásolás)/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Mohamed bin Zayed Species Conservation Fund//12252178/
dc.relation.projectID info:eu-repo/grantAgreement/MEXT//15K07562/
dc.rights.accessRights Abierto es_ES
dc.date.embargoEndDate 2019-06-01 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Müller, T.; Matsubara, H.; Kubara, Y.; Horváth, Á.; Kolics, B.; Taller, J.; Stéger, V.... (2018). Testing cryopreserved European eel sperm for hybridization (A. japonica × A. anguilla). Theriogenology. 113:153-158. https://doi.org/10.1016/j.theriogenology.2018.02.021 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.theriogenology.2018.02.021 es_ES
dc.description.upvformatpinicio 153 es_ES
dc.description.upvformatpfin 158 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 113 es_ES
dc.relation.pasarela S\354127 es_ES
dc.contributor.funder Ministry of Education, Culture, Sports, Science and Technology, Japón
dc.contributor.funder Tokyo University of Agriculture and Technology
dc.contributor.funder Mohamed bin Zayed Species Conservation Fund
dc.contributor.funder National Research, Development and Innovation Office, Hungría
dc.contributor.funder European Commission
dc.contributor.funder European Social Fund
dc.contributor.funder Japan Society for the Promotion of Science


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