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Chondrocytes cultured in an adhesive macroporous scaffold subjected to stirred flow bioreactor behave like in static culture

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Chondrocytes cultured in an adhesive macroporous scaffold subjected to stirred flow bioreactor behave like in static culture

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dc.contributor.author Gamboa Martínez, Tatiana Carolina es_ES
dc.contributor.author Ródenas Rochina, Joaquín es_ES
dc.contributor.author Rico Tortosa, Patricia María es_ES
dc.contributor.author Lebourg, Myriam Madeleine es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.contributor.author Gallego Ferrer, Gloria es_ES
dc.date.accessioned 2018-07-16T07:03:32Z
dc.date.available 2018-07-16T07:03:32Z
dc.date.issued 2013 es_ES
dc.identifier.issn 2157-9083 es_ES
dc.identifier.uri http://hdl.handle.net/10251/105869
dc.description.abstract [EN] The aim of this study was to evaluate whether mechanical stimulation helps to modulate the redifferentiation of human chondrocytes on adhesive 3D substrates. In a previous work, it was shown that chondrocytes seeded in a non-adherent porous scaffold cluster inside the pores when cultured in a bioreactor and produced extracellular matrix with the components of hyaline cartilage suggesting cellular redifferentiation. The current study followed the same culture protocol for up to 4 weeks using two substrates with good adhesion properties: polycaprolactone (PCL) scaffolds uncoated, and coated with fibrin. The cellular response was characterized by studying the cell-material interaction at short times (6, 24 and 72 h), followed by the use of a biological stirrer to evaluate its effect on the chondrogenesis for 7, 14 and 28 days. The study showed strong cell-substrate interaction in both coated and uncoated scaffolds. In short times, cells adhere to the pore walls, form focal adhesions, develop actin fibers and acquire spread morphology showing the characteristics of a monolayer culture. The use of a bioreactor system was useful for reducing cellular proliferation for up to 14 days but no signs of redifferentiation were found for long culture times. es_ES
dc.description.sponsorship The authors are grateful for the financial support received from the Spanish Ministry through the DPI2010-20399-004-03 project. CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER Actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. We would also like to thank Dr. N. Garcia-Giralt from the Institut Municipal d'Investigacio Medica de l'Hospital del Mar, Barcelona, for supplying chondrocytes. en_EN
dc.language Inglés es_ES
dc.publisher American Scientific Publishers es_ES
dc.relation.ispartof Journal of Biomaterials and Tissue Engineering es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Bioreactor es_ES
dc.subject Chondrocytes es_ES
dc.subject Fibrin coating es_ES
dc.subject Polycaprolactone es_ES
dc.subject Redifferentiation es_ES
dc.subject Scaffold es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.subject.classification TERMODINAMICA APLICADA (UPV) es_ES
dc.subject.classification INGENIERIA QUIMICA es_ES
dc.title Chondrocytes cultured in an adhesive macroporous scaffold subjected to stirred flow bioreactor behave like in static culture es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1166/jbt.2013.1091 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//DPI2010-20399-C04-03/ES/DISEÑO Y FABRICACION DE UNA PLATAFORMA BIOMIMETICA TIPO SCAFFOLD%2FSOPORTE PARA LA REGENERACION DEL CARTILAGO ARTICULAR/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Gamboa Martínez, TC.; Ródenas Rochina, J.; Rico Tortosa, PM.; Lebourg, MM.; Gómez Ribelles, JL.; Salmerón Sánchez, M.; Gallego Ferrer, G. (2013). Chondrocytes cultured in an adhesive macroporous scaffold subjected to stirred flow bioreactor behave like in static culture. Journal of Biomaterials and Tissue Engineering. 3(3):312-319. https://doi.org/10.1166/jbt.2013.1091 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1166/jbt.2013.1091 es_ES
dc.description.upvformatpinicio 312 es_ES
dc.description.upvformatpfin 319 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 3 es_ES
dc.description.issue 3 es_ES
dc.relation.pasarela S\260783 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES


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