Soria, JM.; Sancho-Tello, M.; Garcia Esparza, MA.; Mirabet, V.; Bagan, JV.; Monleón Pradas, M.; Carda, C. (2011). Biomaterials coated by dental pulp cells as substrate for neural stem cell differentiation. Journal of Biomedical Materials Research Part A. 97 A(1):85-92. https://doi.org/10.1002/jbm.a.33032
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/107317
Title:
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Biomaterials coated by dental pulp cells as substrate for neural stem cell differentiation
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Author:
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Soria, Jose Miguel
Sancho-Tello, María
Garcia Esparza, M. Angeles
Mirabet, Vicente
Bagan, Jose Vicente
Monleón Pradas, Manuel
Carda, Carmen
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UPV Unit:
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Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada
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Issued date:
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Abstract:
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[EN] This study is focused on the development of an in vitro hybrid system, consisting in a polymeric biomaterial covered by a dental pulp cellular stroma that acts as a scaffold offering a neurotrophic support for the ...[+]
[EN] This study is focused on the development of an in vitro hybrid system, consisting in a polymeric biomaterial covered by a dental pulp cellular stroma that acts as a scaffold offering a neurotrophic support for the subsequent survival and differentiation of neural stem Cells. In the first place, the behavior of dental pulp stroma on the polymeric biomaterial based on ethyl acrylate and hydroxy ethyl acrylate copolymer was studied. For this purpose, cells from normal human third molars were grown onto 0.5-mm-diameter biomaterial discs. After cell culture, quantification of neurotrophic factors generated by the stromal cells was performed by means of an ELISA assay. In the second place, survival and differentiation of adult murine neural stem cells on the polymeric biomaterials covered by dental pulp stromal cells was studied. The results show the capacity of dental pulp cells to uniformly coat the majority of the material's surface and to secrete neurotrophic factors that become crucial for a subsequent differentiation of neural stem cells. The use of stromal cells cultured on scaffolding biomaterials provides neurotrophic pumps that may suggest new criteria for the design of cell therapy experiments in animal models to assist the repair of lesions in Central Nervous System.
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Subjects:
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Cell culture
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Cell adhesion
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Nerve growth factor
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Neural cell
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Stem cell
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Copyrigths:
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Cerrado |
Source:
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Journal of Biomedical Materials Research Part A. (issn:
1549-3296
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DOI:
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10.1002/jbm.a.33032
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Publisher:
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John Wiley & Sons
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Publisher version:
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https://doi.org/10.1002/jbm.a.33032
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Project ID:
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info:eu-repo/grantAgreement/MICINN//MAT2008-06434/ES/MATERIALES PARA REGENERACION NEURAL Y ANGIOGENESIS EN EL SISTEMA NERVIOSO CENTRAL/
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Thanks:
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Contract grant sponsors: Programa de ayudas a la Investigacion cientifica Raman Areces; convenio de colaboracion entre el Instituto de Salud Carlos III, la Conselleria de Sanidad de la Generalitat Valenciana y la Fundacion ...[+]
Contract grant sponsors: Programa de ayudas a la Investigacion cientifica Raman Areces; convenio de colaboracion entre el Instituto de Salud Carlos III, la Conselleria de Sanidad de la Generalitat Valenciana y la Fundacion de la Comunidad Valenciana Centro de Investigacion Principe Felipe para la investigacion basica y traslacional en medicina regenerative
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Type:
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Artículo
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