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In vitro 3D culture of human chondrocytes using modified epsilon-caprolactone scaffolds with varying hydrophilicity and porosity

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In vitro 3D culture of human chondrocytes using modified epsilon-caprolactone scaffolds with varying hydrophilicity and porosity

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dc.contributor.author Pérez Olmedilla, Marcos es_ES
dc.contributor.author Lebourg, Myriam Madeleine es_ES
dc.contributor.author Escobar Ivirico, Jorge Luis es_ES
dc.contributor.author Nebot, I. es_ES
dc.contributor.author Garcia Giralt, N. es_ES
dc.contributor.author Gallego-Ferrer, Gloria es_ES
dc.contributor.author Soria, J. M. es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.date.accessioned 2018-09-17T06:53:33Z
dc.date.available 2018-09-17T06:53:33Z
dc.date.issued 2012 es_ES
dc.identifier.issn 0885-3282 es_ES
dc.identifier.uri http://hdl.handle.net/10251/107329
dc.description.abstract [EN] Two series of 3D scaffolds based on epsilon-caprolactone were synthesized. The pore size and architecture (spherical interconnected pores) was the same in all the scaffolds. In one of the series of scaffolds, made of pure epsilon-polycaprolactone, the volume fraction of pores varied between 60% and 85% with the main consequence of varying the interconnectivity between pores since the pore size was kept constant. The other scaffolds were prepared with copolymers made of a epsilon-caprolactone-based hydrophobous monomer and hydroxyethyl acrylate, as the hydrophilic component. Thus, the hydrophilicity and, presumably, the adhesion properties varied monotonously in the copolymer series while porosity was kept constant. A suspension of human chondrocytes in culture medium was injected in the 3D scaffolds and cultured in static conditions up to 28 days. SEM and immunofluorescence assays allowed characterizing cells and extracellular matrix inside the scaffolds after different culture times. To do that, cross sections of the scaffolds were observed by SEM and confocal microscopy. The quantity of cells inside the scaffolds decreases with a decrease of the volume fraction of pores, due to the lack of interconnectivity between the cavities. The scaffolds up to a 30% of hydrophilicity behave in a similar way than the hydrophobous; a further increase of the hydrophilicity rapidly decreases cell viability. In all the experiments production of collagen type I, type II, and aggrecan was found, and some cells were Ki-67 positive, showing that some cells are adhered to the pore walls and maintain their dedifferentiated phenotype even when cultured in three-dimensional conditions. es_ES
dc.description.sponsorship GGF and JLGR acknowledge funding in the framework of the collaboration agreement among the Instituto de Salud Carlos III, the Conselleria de Sanidad de la Comunidad Valenciana and the CIPF for the 'Investigacion Basica y Traslacional en Medicina Regenerativa' en_EN
dc.language Inglés es_ES
dc.publisher SAGE Publications es_ES
dc.relation.ispartof Journal of Biomaterials Applications es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Scaffold es_ES
dc.subject Tissue engineering es_ES
dc.subject Biodegradable es_ES
dc.subject Poly(epsilon-caprolactone) es_ES
dc.subject Chondrocyte es_ES
dc.subject Cartilage es_ES
dc.subject Proliferation es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title In vitro 3D culture of human chondrocytes using modified epsilon-caprolactone scaffolds with varying hydrophilicity and porosity es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1177/0885328211404263 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MEC//MAT2007-66759-C03-01/ES/NUEVOS SUBSTRATOS POLIMERICOS BIORREABSORBIBLES PARA LA REGENERACION DEL CARTILAGO ARTICULAR/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Pérez Olmedilla, M.; Lebourg, MM.; Escobar Ivirico, JL.; Nebot, I.; Garcia Giralt, N.; Gallego-Ferrer, G.; Soria, JM.... (2012). In vitro 3D culture of human chondrocytes using modified epsilon-caprolactone scaffolds with varying hydrophilicity and porosity. Journal of Biomaterials Applications. 27(3):299-309. https://doi.org/10.1177/0885328211404263 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1177/0885328211404263 es_ES
dc.description.upvformatpinicio 299 es_ES
dc.description.upvformatpfin 309 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 27 es_ES
dc.description.issue 3 es_ES
dc.relation.pasarela S\222518 es_ES
dc.contributor.funder Ministerio de Educación y Ciencia es_ES


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