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2b or not 2b: experimental evolution of functional exogenous sequences in a plant RNA virus

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2b or not 2b: experimental evolution of functional exogenous sequences in a plant RNA virus

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dc.contributor.author Willemsen, Anouk es_ES
dc.contributor.author Zwart, Mark Peter es_ES
dc.contributor.author Ambros Palaguerri, Silvia es_ES
dc.contributor.author Carrasco Jiménez, José Luis es_ES
dc.contributor.author Elena Fito, Santiago Fco es_ES
dc.date.accessioned 2018-09-25T07:15:03Z
dc.date.available 2018-09-25T07:15:03Z
dc.date.issued 2017 es_ES
dc.identifier.issn 1759-6653 es_ES
dc.identifier.uri http://hdl.handle.net/10251/108070
dc.description.abstract [EN] Horizontal gene transfer (HGT) is pervasive in viruses and thought to be a key mechanism in their evolution. On the other hand, strong selective constraints against increasing genome size are an impediment for HGT, rapidly purging horizontally transferred sequences and thereby potentially hindering evolutionary innovation. Here, we explore experimentally the evolutionary fate of viruses with simulated HGT events, using the plant RNA virus Tobacco etch virus (TEV), by separately introducing two functional, exogenous sequences to its genome. One of the events simulates the acquisition of a new function though HGT of a conserved AlkB domain, responsible for the repair of alkylation or methylation damage in many organisms. The other event simulates the acquisition of a sequence that duplicates an existing function, through HGT of the 2b RNA silencing suppressor from Cucumber mosaic virus. We then evolved these two viruses, tracked the maintenance of the horizontally transferred sequences over time, and for the final virus populations, sequenced their genome and measured viral fitness. We found that the AlkB domain was rapidly purged from the TEV genome, restoring fitness to wild-type levels. Conversely, the 2b gene was stably maintained and did not have a major impact on viral fitness. Moreover, we found that 2b is functional in TEV, as it provides a replicative advantage when the RNA silencing suppression domain of HC-Pro is mutated. These observations suggest a potentially interesting role for HGT of short functional sequences in ameliorating evolutionary constraints on viruses, through the duplication of functions. es_ES
dc.description.sponsorship We thank Francisca de la Iglesia and Paula Agudo for excellent technical assistance. This work was supported by the John Templeton Foundation (grant 22371), the European Commission 7th Framework Program EvoEvo Project (grant ICT-610427), and the Spanish Ministerio de Economia y Competitividad (grants BFU2012-30805 and BFU2015-65037-P) to S.F.E. The opinions expressed in this publication are those of the authors and do not necessarily reflect the views of the John Templeton Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.language Inglés es_ES
dc.publisher Oxford University Press es_ES
dc.relation John Templeton Foundation /JTF22371 es_ES
dc.relation MINECO/BFU2012-30805 es_ES
dc.relation MINECO/BFU2015-65037-P es_ES
dc.relation.ispartof Genome Biology and Evolution es_ES
dc.rights Reconocimiento - No comercial (by-nc) es_ES
dc.subject Horizontal gene transfer es_ES
dc.subject Virus evolution es_ES
dc.subject Genome evolution es_ES
dc.subject Experimental evolution es_ES
dc.title 2b or not 2b: experimental evolution of functional exogenous sequences in a plant RNA virus es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/gbe/evw300 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/610427/EU es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Willemsen, A.; Zwart, MP.; Ambros Palaguerri, S.; Carrasco Jiménez, JL.; Elena Fito, SF. (2017). 2b or not 2b: experimental evolution of functional exogenous sequences in a plant RNA virus. Genome Biology and Evolution. 9(2):297-310. https://doi.org/10.1093/gbe/evw300 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1093/gbe/evw300 es_ES
dc.description.upvformatpinicio 297 es_ES
dc.description.upvformatpfin 310 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 9 es_ES
dc.description.issue 2 es_ES
dc.identifier.pmid 28137747
dc.identifier.pmcid PMC5381683
dc.relation.pasarela S\339110 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder John Templeton Foundation es_ES


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