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Long -term feeding with high plant protein based diets in gilthead seabream (Sparus aurata, L.) leads to changes in the inflammatory and immune related gene expression at intestinal level

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Long -term feeding with high plant protein based diets in gilthead seabream (Sparus aurata, L.) leads to changes in the inflammatory and immune related gene expression at intestinal level

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dc.contributor.author Estruch-Cucarella, Guillem es_ES
dc.contributor.author Collado, Mª Carmen es_ES
dc.contributor.author Monge-Ortiz, Raquel es_ES
dc.contributor.author Tomas-Vidal, A. es_ES
dc.contributor.author Jover Cerdá, Miguel es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Perez Martinez, Gaspar es_ES
dc.contributor.author Martínez-Llorens, Silvia es_ES
dc.date.accessioned 2018-10-19T04:32:19Z
dc.date.available 2018-10-19T04:32:19Z
dc.date.issued 2018 es_ES
dc.identifier.uri http://hdl.handle.net/10251/110908
dc.description.abstract [EN] Background: In order to ensure sustainability of aquaculture production of carnivourous fish species such as the gilthead seabream (Sparus aurata, L.), the impact of the inclusion of alternative protein sources to fishmeal, including plants, has been assessed. With the aim of evaluating long-term effects of vegetable diets on growth and intestinal status of the on-growing gilthead seabream (initial weight = 129 g), three experimental diets were tested: a strict plant protein-based diet (VM), a fishmeal based diet (FM) and a plant protein-based diet with 15% of marine ingredients (squid and krill meal) alternative to fishmeal (VM+). Intestines were sampled after 154 days. Besides studying growth parameters and survival, the gene expression related to inflammatory response, immune system, epithelia integrity and digestive process was analysed in the foregut and hindgut sections, as well as different histological parameters in the foregut. Results: There were no differences in growth performance (p = 0.2703) and feed utilization (p = 0.1536), although a greater fish mortality was recorded in the VM group (p = 0.0141). In addition, this group reported a lower expression in genes related to pro-inflammatory response, as Interleukine-1 beta (il1 beta, p = 0.0415), Interleukine-6 (il6, p = 0.0347) and cyclooxigenase-2 (cox2, p = 0.0014), immune-related genes as immunoglobulin M (igm, p = 0.0002) or bacterial defence genes as alkaline phosphatase (alp, p = 0.0069). In contrast, the VM+ group yielded similar survival rate to FM (p = 0.0141) and the gene expression patterns indicated a greater induction of the inflammatory and immune markers (il1 beta, cox2 and igm). However, major histological changes in gut were not detected. Conclusions: Using plants as the unique source of protein on a long term basis, replacing fishmeal in aqua feeds for gilthead seabream, may have been the reason of a decrease in the level of different pro-inflammatory mediators (il1 beta, il6 and cox2) and immune-related molecules (igm and alp), which reflects a possible lack of local immune response at the intestinal mucosa, explaining the higher mortality observed. Krill and squid meal inclusion in vegetable diets, even at low concentrations, provided an improvement in nutrition and survival parameters compared to strictly plant protein based diets as VM, maybe explained by the maintenance of an effective immune response throughout the assay. es_ES
dc.description.sponsorship The research has been partially funded by Vicerrectorat d'Investigacio, Innovacio i Transferencia of the Universitat Politecnica de Valencia, which belongs to the project Aquaculture feed without fishmeal (SP20120603). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.
dc.language Inglés es_ES
dc.publisher Springer (Biomed Central Ltd.) es_ES
dc.relation info:eu-repo/grantAgreement/MECD//FPU13%2F01278/ES/FPU13%2F01278/ es_ES
dc.relation UPV-VIN/SP20120603 es_ES
dc.relation.ispartof BMC Veterinary Research es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Gilthead seabream es_ES
dc.subject Vegetable meal es_ES
dc.subject Squid meal es_ES
dc.subject Krill meal es_ES
dc.subject Intestine es_ES
dc.subject Histology es_ES
dc.subject Gene expression es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.title Long -term feeding with high plant protein based diets in gilthead seabream (Sparus aurata, L.) leads to changes in the inflammatory and immune related gene expression at intestinal level es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12917-018-1626-6 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Estruch-Cucarella, G.; Collado, MC.; Monge-Ortiz, R.; Tomas-Vidal, A.; Jover Cerda, M.; Peñaranda, D.; Pérez Martínez, G.... (2018). Long -term feeding with high plant protein based diets in gilthead seabream (Sparus aurata, L.) leads to changes in the inflammatory and immune related gene expression at intestinal level. BMC Veterinary Research. 14. doi:10.1186/s12917-018-1626-6 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1186/s12917-018-1626-6 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 14 es_ES
dc.identifier.eissn 1746-6148 es_ES
dc.identifier.pmid 30285734
dc.identifier.pmcid PMC6171182
dc.relation.pasarela S\369342 es_ES
dc.contributor.funder Ministerio de Educación es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
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