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METHANOGENESIS IN RABBIT CAECUM AS AFFECTED BY THE FERMENTATION PATTERN: IN VITRO AND IN VIVO MEASUREMENTS

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METHANOGENESIS IN RABBIT CAECUM AS AFFECTED BY THE FERMENTATION PATTERN: IN VITRO AND IN VIVO MEASUREMENTS

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dc.contributor.author Belenguer, A. es_ES
dc.contributor.author Fondevila, M. es_ES
dc.contributor.author Balcells, J. es_ES
dc.contributor.author Abecia, L. es_ES
dc.contributor.author Lachica, M. es_ES
dc.contributor.author Carro, M.D. es_ES
dc.date.accessioned 2011-07-08T11:20:33Z
dc.date.issued 2011-07
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/11150
dc.description.abstract [EN] Methane formation and caecal fermentation patterns were studied in vivo and in vitro in 16 white New Zealand rabbits (70-80 d and 2.27 ± 0.064 kg) allocated to four diets formulated to have a similar neutral detergent fibre (33.8±0.53%) and protein (17.7±0.33%) content, with two different fibre sources (alfalfa hay, AH or sugar beet pulp, SP) and starch (wheat or maize). Animals received the diet for 16 to 20 days before methane production was measured in vivo in a respiratory chamber. Animals were subsequently slaughtered at approximately 9:00 and caecal contents were sampled and used as inoculum for in vitro incubations to determine gas and methane production. Volatile fatty acid (VFA) and purine base (PB) concentrations were determined from both caecal content and incubation medium after 6 h. Total VFA concentration in caecal content decreased (P<0.05) in rabbits fed AH-maize diet compared with rabbits fed AH-wheat and SP-maize diets (37.7 vs. 59.6 mM), with those fed SP-wheat showing an intermediate value (53.0 mM). Fermentation pattern was affected when maize was the source of starch compared to wheat, with lower acetate (0.72 vs. 0.79; P<0.01) and higher butyrate (0.19 vs. 0.14; P<0.001) molar proportions. Fermentation in vivo vs. in vitro showed some differences (molar proportions of acetate, 0.76 vs. 0.73, P<0.001, and propionate, 0.069 vs. 0.091, P<0.001, in vivo and in vitro, respectively), probably due to differences in pH (6.0 vs. 6.7 in vivo and in vitro; P<0.001). Only 2 out of 16 rabbits produced a substantial volume of methane in vivo (on average, 12.6 ml/BW0.75/d or 0.56 mmol/BW0.75/d), showing a high inter-individual variability that hindered comparison of treatment differences. In contrast, methane was detected in vitro in all cases and volumes were more homogenous, a higher formation (P<0.05) being observed with maize compared to wheat. A similar effect was shown in total gas production. The low methane production and H2 recovery suggest the importance of H2 disposal mechanisms other than methanogenesis, such as reductive acetogenesis. PB concentration in caecal content and the incubation medium, as an index of microbial concentration, was highest when SP was added with maize (P<0.05). es_ES
dc.description.sponsorship For the realisation of this work, A. Belenguer and L. Abecia received grants from the Spanish Ministry of Education and Science and the Basque Government, respectively. This work was financed by the Diputación General de Aragón through project DGA PM095/2006.
dc.language Inglés es_ES
dc.publisher World Rabbit Science. ICTA. UPV es_ES
dc.relation DGA/PM095/2006
dc.relation.ispartof World Rabbit Science
dc.rights Reserva de todos los derechos es_ES
dc.subject Rabbit es_ES
dc.subject Caecal fermentation es_ES
dc.subject Methanogenesis es_ES
dc.subject Reductive acetogenesi es_ES
dc.title METHANOGENESIS IN RABBIT CAECUM AS AFFECTED BY THE FERMENTATION PATTERN: IN VITRO AND IN VIVO MEASUREMENTS es_ES
dc.type Artículo es_ES
dc.date.updated 2011-07-08T07:48:12Z
dc.identifier.doi 10.4995/wrs.2011.826
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Belenguer, A.; Fondevila, M.; Balcells, J.; Abecia, L.; Lachica, M.; Carro, M. (2011). METHANOGENESIS IN RABBIT CAECUM AS AFFECTED BY THE FERMENTATION PATTERN: IN VITRO AND IN VIVO MEASUREMENTS. World Rabbit Science. 19(2):75-83. doi:10.4995/wrs.2011.826 es_ES
dc.relation.publisherversion http://doi.org/10.4995/wrs.2011.826
dc.description.upvformatpinicio 75
dc.description.upvformatpfin 83
dc.description.volume 19
dc.description.issue 2
dc.identifier.eissn  1989-8886
dc.identifier.eissn 1989-8886 es_ES
dc.contributor.funder Diputación General de Aragón (DGA)


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