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Biological activity of the substrate-induced fibronectin network: Insight into the third dimension through electrospun fibers

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Biological activity of the substrate-induced fibronectin network: Insight into the third dimension through electrospun fibers

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dc.contributor.author Gugutkov, Dencho es_ES
dc.contributor.author González-García, Cristina es_ES
dc.contributor.author Rodríguez Hernández, José Carlos es_ES
dc.contributor.author Altankov, George es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.date.accessioned 2018-11-08T05:34:37Z
dc.date.available 2018-11-08T05:34:37Z
dc.date.issued 2009 es_ES
dc.identifier.issn 0743-7463 es_ES
dc.identifier.uri http://hdl.handle.net/10251/112087
dc.description.abstract [EN] Fibronectin (FN) fibrillogenesis is a cell-mediated process involving integrin activation that results in conformational changes of FN molecules and the organization of actin cytoskeleton. A similar process can be induced by some chemistries in the absence of cells, e.g., poly(ethyl acrylate) (PEA), which enhance FN-FN interactions leading to the formation of a biologically active network. Atomic force microscopy images of single FN molecules, at the early stages of adsorption on plane PEA, allow one to rationalize the process. Further, the role of the spatial organization of the FN network on the cellular response is investigated through its adsorption on electrospun fibers. Randomly oriented and aligned PEA fibers were prepared to mimic the three-dimensional organization of the extracellular matrix. The formation of the FN network on the PEA fibers but not on the supporting coverglass was confirmed. Fibroblasts aligned with oriented fibers, displayed extended morphology, developed linearly organized focal adhesion complexes, and matured actin filaments. Conversely, oil random PEA fibers, cells acquired polygonal morphology with altered actin cytoskeleton but well-developed focal adhesions. Late FN matrix formation was also influenced: spatially organized FN matrix fibrils along the oriented PEA fibers and an altered arrangement on random ones, es_ES
dc.description.sponsorship AFM was performed tinder the technical guidance of the Microscopy Service at the Universidad Politecnica de Valencia, whose advice is greatly appreciated. The support of the Spanish Ministry of Science through project MAT200608120 (including the FEDER financial support) is kindly acknowledged. This work has been partly financed through 'convenio de colaboracion entre el Instituto de Salud Carlos III, la Conselleria de Sanidad de la Generalitat Valenciana y la Fundacion de la Comunidad Valenciana Centro de Investigacion Principe Felipe para la investigacion basica y traslacional en medicina regenerativa'. en_EN
dc.language Inglés es_ES
dc.publisher American Chemical Society es_ES
dc.relation MINISTERIO DE EDUCACION /MAT2006-08120 es_ES
dc.relation.ispartof Langmuir es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.subject.classification TERMODINAMICA APLICADA (UPV) es_ES
dc.title Biological activity of the substrate-induced fibronectin network: Insight into the third dimension through electrospun fibers es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1021/la9012203 es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Gugutkov, D.; González-García, C.; Rodríguez Hernández, JC.; Altankov, G.; Salmerón Sánchez, M. (2009). Biological activity of the substrate-induced fibronectin network: Insight into the third dimension through electrospun fibers. Langmuir. 25(18):10893-10900. doi:10.1021/la9012203 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1021/la9012203 es_ES
dc.description.upvformatpinicio 10893 es_ES
dc.description.upvformatpfin 10900 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 25 es_ES
dc.description.issue 18 es_ES
dc.relation.pasarela S\36715 es_ES
dc.contributor.funder Ministerio de Educación es_ES


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