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Della protein function during differential growth processes in arabidopsis

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Della protein function during differential growth processes in arabidopsis

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dc.contributor.advisor Blazquez Rodriguez, Miguel Angel es_ES
dc.contributor.advisor Alabadí Diego, David es_ES
dc.contributor.author Gallego Bartolomé, Javier es_ES
dc.date.accessioned 2011-08-01T17:19:27Z
dc.date.available 2011-08-01T17:19:27Z
dc.date.created 2011-06-27T08:00:00Z es_ES
dc.date.issued 2011-08-01T17:19:20Z es_ES
dc.identifier.uri http://hdl.handle.net/10251/11403
dc.description.abstract The plant hormones gibberellins (GAs) regulate multiple processes of plant development. Most of this regulation occurs at the transcriptional level, through the activity of the DELLAs, which are nuclear-localized proteins subjected to GA-mediated proteolitic degradation. DELLAs are encoded by five genes, and genetic studies show that each DELLA displays specific, but also partially overlapping roles with respect to their paralogs. In this Thesis, we have addressed two issues: (1) the contribution of DELLA multiplication to the diversification of functions controlled by GAs; and (2) the identification of direct targets regulated by DELLAs in etiolated seedlings with special attention to those involved in differential growth processes. Using combinations of mutants and transgenic lines expressing two phylogenetically distant DELLA genes (RGA and RGL2), we have found that these two DELLA proteins can perform each other's role as long as they are expressed under the reciprocal promoters, indicating that DELLA subfunctionalization relies mainly on their differential expression patterns. To identify direct DELLA targets, we have performed transcriptomic analyses of dark-grown seedlings expressing an inducible version of gai-1, a stable, dominant allele of a DELLA gene. This approach rendered a list of over 150 genes differentially expressed after induction of gai-1. The presence of several auxin-related genes among the primary targets of DELLA proteins has allowed us to establish a new role for GAs in the modulation of hypocotyl gravitropism through the repression of IAA19/MASSUGU2 expression by DELLAs. Moreover, the repression of HOOKLESS1 and the auxin efflux carriers PIN3 and PIN7 by DELLAs, is proposed as the molecular mechanism to explain the already known physiological regulation of apical hook development by GAs. es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.rights Reserva de todos los derechos es_ES
dc.source Riunet es_ES
dc.subject Gibberellin es_ES
dc.subject Arabidopsis es_ES
dc.subject Auxin es_ES
dc.subject Tropism es_ES
dc.subject Hook es_ES
dc.subject Della es_ES
dc.title Della protein function during differential growth processes in arabidopsis
dc.type Tesis doctoral es_ES
dc.identifier.doi 10.4995/Thesis/10251/11403 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Gallego Bartolomé, J. (2011). Della protein function during differential growth processes in arabidopsis [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/11403 es_ES
dc.description.accrualMethod Palancia es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.relation.tesis 3582 es_ES


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