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Successful development of vitrified embryonic kidney after laparoscopy transplantation into non-immunosuppressed hosts

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Successful development of vitrified embryonic kidney after laparoscopy transplantation into non-immunosuppressed hosts

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dc.contributor.author Garcia-Dominguez, X es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.contributor.author Vera Donoso, Cesar David es_ES
dc.contributor.author Marco-Jiménez, Francisco es_ES
dc.date.accessioned 2018-12-19T21:05:58Z
dc.date.available 2018-12-19T21:05:58Z
dc.date.issued 2017 es_ES
dc.identifier.uri http://hdl.handle.net/10251/114243
dc.description.abstract [EN] Transplantation from living or deceased donors has been limited by donor availability that is opposed to the increasing demand and by the risk of allograft loss rejection and immunosuppressive therapy toxicity. In recent years, xenotransplantation of metanephroi has offered a novel solution for the unlimited supply of human donor organs. However, even if in a most favourable and idyllic situation the organ availability and its demand could be balanced using transplantation of animal embryonic organs, the future of this treatment would still be compromised without proper long-term storage procedure. Thus, based on the ongoing long-term storage necessities, this study was designed to investigate the effect of two specific time window of the metanephroi development (15 days-old and 16 days-old) on the in vivo developmental capacity and the developed morphologically normal glomeruli of vitrified metanephroi in non-immunosuppressive rabbits. Metanephroi originating from 15 and 16 days old rabbit embryos were vitrified using M22 solution and Cryotop® as a device. After three months of storage in liquid nitrogen, metanephroi were transplanted into non-immunosuppressed adult hosts by laparoscopy surgery. Twenty-one days after allotransplantation, 6 (32%) and 7 (35%) new kidneys were recovered. All the new kidneys recovered exhibited significant growth and mature glomeruli. However, histomorphometry analysis revealed that new kidneys developed from 16 days-old metanephroi exhibit a greater degree of maturity compared with 15 days-old metanephroi. Results obtained in the present study point out that, in rabbit model, vitrified 16 days-old metanephroi could be stored in liquid nitrogen, achieving good in vivo developmental capacity and the developed morphologically normal glomeruli after laparoscopy transplantation into non-immunosuppressed hosts. es_ES
dc.description.sponsorship This work was supported by funds from the Generalitat Valenciana Research Programme (PrometeoII 2014/036), ALCER-TURIA foundation and PRECIPITA CROWDFUNDING. English text version was revised by N. Macowan English Language Service.
dc.language Inglés es_ES
dc.publisher Open Access Text es_ES
dc.relation GV/PROMETEOII/2014/036 es_ES
dc.relation.ispartof Transplantation open es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Metanephros es_ES
dc.subject Cryopreservtion es_ES
dc.subject Xenotransplant es_ES
dc.subject Kidney disease es_ES
dc.subject Cryobanks es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.title Successful development of vitrified embryonic kidney after laparoscopy transplantation into non-immunosuppressed hosts es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.15761/JTO.1000125 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Garcia-Dominguez, X.; Vicente Antón, JS.; Vera Donoso, CD.; Marco-Jiménez, F. (2017). Successful development of vitrified embryonic kidney after laparoscopy transplantation into non-immunosuppressed hosts. Transplantation open. 2(2):1-5. doi:10.15761/JTO.1000125 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.15761/JTO.1000125 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 5 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 2 es_ES
dc.description.issue 2 es_ES
dc.identifier.eissn 2399-5289 es_ES
dc.relation.pasarela S\334130 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Asociación para la lucha contra las enfermedades del riñón es_ES


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