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dc.contributor.author | Jimenez-Meneses, P. | es_ES |
dc.contributor.author | Bañuls Polo, María-José | es_ES |
dc.contributor.author | Puchades, Rosa | es_ES |
dc.contributor.author | Maquieira Catala, Angel | es_ES |
dc.date.accessioned | 2019-02-21T21:03:31Z | |
dc.date.available | 2019-02-21T21:03:31Z | |
dc.date.issued | 2018 | es_ES |
dc.identifier.issn | 0003-2700 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/117074 | |
dc.description.abstract | [EN] Spatially controlled anchoring of NA probes onto microscope glass slides by a novel fluor-thiol coupling reaction is performed. By this UV-initiated reaction, covalent immobilization in very short times (30 s at 254 nm) is achieved with probe densities of up to 39.6 pmol/cm(2). Modulating the surface hydrophobicity by combining a hydrophobic silane and a hydrophilic silane allows the fabrication of tuned surfaces where the analyte approaches only the anchored probe, which notably reduces nonspecific adsorption and the background. The generated substrates have proven clear advantages for discriminating single-base-pair mismatches, and for detecting bacterial PCR products. The hybridization sensitivity achieved by these high-performance surfaces is about 1.7 pM. Finally, this anchoring reaction is demonstrated using two additional surfaces: polytetrafluoroethylene (PTFE) and polyvinylidene fluoride (PVDF) membranes. This provides a very interesting pathway for anchoring thiolated biomolecules onto surfaces with C-F motifs via a quick clean UV reaction. | es_ES |
dc.description.sponsorship | Financial support from INTERBOINTER (project CTQ2013-45875-R) and BIHOLOG (Project CTQ2016-75749-R), FEDER, and GVA PROMETEO II 2014/040 is acknowledged. The authors also thank Dr. Tortajada-Genaro and Dr. Ninoles Rodenes for providing the Salmonella and Campylobacter PCR-amplified products. P.J.-M. acknowledges the Spanish Ministry of Economy, Industry and Competitiveness for the public FPI grant (Project CTQ2013-45875-R) and the cofinancing by, the European Social Fund. Dr. Miguel Angel Gonzalez-Martinez, Dr. Sergio Navalon, and Dr. Patricia Concepcion from Universitat Politecnica de Valencia are acknowledged for their help in the XPS analysis. | |
dc.language | Inglés | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.relation.ispartof | Analytical Chemistry | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | Fluor-thiol Photocoupling Reaction for Developing High Performance Nucleic Acid (NA) Microarrays | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1021/acs.analchem.8b00265 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CTQ2016-75749-R/ES/BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CTQ2013-45875-R/ES/BIOSENSADO EN SOPORTES INTERACTIVOS CON PROPIEDADES INTERFEROMETRICAS PARA APLICACIONES CLINICAS. DEMOSTRACION EN FARMACOGENETICA Y ALERGIAS MEDICAMENTOSAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F040/ES/Estudio de estrategias fisico-químicas para el desarrollo de biosensores interferométricos en soportes interactivos de aplicación en clínica/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.date.embargoEndDate | 2019-10-02 | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.description.bibliographicCitation | Jimenez-Meneses, P.; Bañuls Polo, M.; Puchades, R.; Maquieira Catala, A. (2018). Fluor-thiol Photocoupling Reaction for Developing High Performance Nucleic Acid (NA) Microarrays. Analytical Chemistry. 90(19):11224-11231. https://doi.org/10.1021/acs.analchem.8b00265 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://doi.org/10.1021/acs.analchem.8b00265 | es_ES |
dc.description.upvformatpinicio | 11224 | es_ES |
dc.description.upvformatpfin | 11231 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 90 | es_ES |
dc.description.issue | 19 | es_ES |
dc.identifier.pmid | 30178656 | |
dc.relation.pasarela | S\368603 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |