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Modulating receptor-ligand binding in biorecognition by setting surface wettability

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Modulating receptor-ligand binding in biorecognition by setting surface wettability

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dc.contributor.author Aragón Revuelta, Pilar es_ES
dc.contributor.author Noguera Murray, Patricia Silvestre es_ES
dc.contributor.author Bañuls Polo, María-José es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Catala, Angel es_ES
dc.contributor.author González Martínez, Miguel Ángel es_ES
dc.date.accessioned 2019-02-21T21:03:43Z
dc.date.available 2019-02-21T21:03:43Z
dc.date.issued 2018 es_ES
dc.identifier.issn 1618-2642 es_ES
dc.identifier.uri http://hdl.handle.net/10251/117076
dc.description.abstract [EN] Modulation of support wettability used for microarray format biosensing has led to an improvement of results. Hydrophobicity of glass chips was set by derivatizing with single vinyl organosilanes of different chain length and silane mixtures. Thiol-ene photochemical linking has been used as effective chemistry for covalent anchoring of thiolated probes. Lowest unspecific binding and highest signal intensity and SNR were obtained with large hydrocarbon chain (C-22) silanes or a shorter one (C-10) containing fluorine atoms. SNR resulting values are improved, reaching levels higher than 1500 in some cases, when using vinyl silanes modified with 1% C-10 alkyl fluorinated one, because mild hydrophobicity was achieved (water contact angle ca. 110 degrees) for all silanes, including the short C-2 and C-3, thus giving rise to smaller and better defined array spots. In addition, unspecific binding of reagents and targets was totally withdrawn. Hence, good-performing surfaces for biosensing applications can be built using appropriate organosilane reagent selection, including fluorinated ones. es_ES
dc.description.sponsorship We thank Patricia Concepcion and Dolores Soriano (Instituto de Tecnologia Quimica, Universitat Politecnica de Valencia-Consejo Superior de Investigaciones Cientificas) for helping with XPS analysis. Financial support from the Generalitat Valenciana (GVA-PROMETEO/2014/040), FEDER, and the Spanish Ministry of Economy and Competitiveness (CTQ2013-45875-R and CTQ2016-75749-R) is acknowledged.
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Analytical and Bioanalytical Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Surface wettability es_ES
dc.subject Organosilane es_ES
dc.subject Probe anchoring es_ES
dc.subject Thiol-ene click reaction es_ES
dc.subject Biosensing es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Modulating receptor-ligand binding in biorecognition by setting surface wettability es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00216-018-1247-8 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F040/ES/Estudio de estrategias fisico-químicas para el desarrollo de biosensores interferométricos en soportes interactivos de aplicación en clínica/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CTQ2016-75749-R/ES/BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CTQ2013-45875-R/ES/BIOSENSADO EN SOPORTES INTERACTIVOS CON PROPIEDADES INTERFEROMETRICAS PARA APLICACIONES CLINICAS. DEMOSTRACION EN FARMACOGENETICA Y ALERGIAS MEDICAMENTOSAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.date.embargoEndDate 2019-09-01 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Aragón Revuelta, P.; Noguera Murray, PS.; Bañuls Polo, M.; Puchades, R.; Maquieira Catala, A.; González Martínez, MÁ. (2018). Modulating receptor-ligand binding in biorecognition by setting surface wettability. Analytical and Bioanalytical Chemistry. 410(23):5723-5730. https://doi.org/10.1007/s00216-018-1247-8 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1007/s00216-018-1247-8 es_ES
dc.description.upvformatpinicio 5723 es_ES
dc.description.upvformatpfin 5730 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 410 es_ES
dc.description.issue 23 es_ES
dc.identifier.pmid 30019083
dc.relation.pasarela S\366792 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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