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dc.contributor.author | Godoy-Reyes, Tania Mariel![]() |
es_ES |
dc.contributor.author | Llopis-Lorente, Antoni![]() |
es_ES |
dc.contributor.author | Costero, Ana M.![]() |
es_ES |
dc.contributor.author | Sancenón, Félix![]() |
es_ES |
dc.contributor.author | Gaviña, Pablo![]() |
es_ES |
dc.contributor.author | Martínez-Máñez, Ramón![]() |
es_ES |
dc.date.accessioned | 2019-02-23T21:06:12Z | |
dc.date.available | 2019-02-23T21:06:12Z | |
dc.date.issued | 2018 | es_ES |
dc.identifier.issn | 0925-4005 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/117234 | |
dc.description.abstract | [EN] We report a simple, sensitive and selective method for the colorimetric detection of serotonin (5-HT) in aqueous media using bifunctionalized gold nanoparticles (AuNPs). The probe (1) consisted of AuNPs functionalised with dithiobis(succinimidylpropionate) (DSP) and N-acetyl-l-cysteine (NALC). DSP was chosen to react with the amino group of 5-HT, whereas NALC was chosen to bind the hydroxyl group in 5-HT through hydrogen bonding and electrostatic interactions. A double interaction between nanoparticles and the hydroxyl and the amino group of serotonin led to interparticle-crosslinking aggregation. This, resulted in a colour change from red to blue that can be observed by the naked eye. The probe was selective to 5-HT and no colour modulation was observed in the presence of other neurotransmitters (i.e. dopamine, epinephrine, norepinephrine), selected biomolecules (i.e. L-tyrosine, gamma-aminobutyric acid, L-cysteine, uric acid, oxalic acid, aspartic acid and glutamic acid) and common inorganic species. A limit of detection as low as 0.1 mu M was determined in buffered water at pH 7 by UV-vis titrations. Similar response of the probe to 5-HT was observed in simulated blood serum, with a limit of detection of 0.12 mu M, and a linear response within the 0-3 mu M concentration range, which is within the range of the 5-HT concentrations of clinical interest. Finally, the performance of probe (1) in real human blood samples was evaluated, and showed a remarkable ability to distinguish between normal 5-HT levels and 5-HT levels indicative of disease. (c) 2017 Elsevier B.V. All rights reserved. | es_ES |
dc.description.sponsorship | Financial support from the Spanish Government (Projects MAT2015-64139-C4-1-R and MAT2015-64139-C4-4-R) and the Generalitat Valenciana (Project PROMETEOII/2014/047) is gratefully acknowledged. T. Godoy-Reyes is grateful to the Generalitat Valenciana for her Santiago Grisolia fellowship. A. Llopis-Lorente thanks "La Caixa" Banking Foundation for his PhD grant. SCSIE (Universitat de Valencia) is gratefully acknowledged for all the equipment employed. NMR was registered at the U26 facility of ICTS "NANBIOSIS" at the Universitat de Valencia. Support from the Juana Portaceli Health Centre of the Universitat Politecnica de Valencia to obtain blood samples from a healthy volunteer is gratefully acknowledged. | |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Sensors and Actuators B Chemical | es_ES |
dc.rights | Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) | es_ES |
dc.subject | Serotonin (5-HT) | es_ES |
dc.subject | Gold nanoparticles | es_ES |
dc.subject | Colorimetric detection | es_ES |
dc.subject | Aggregation | es_ES |
dc.subject | Neurotransmitters | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.subject.classification | QUIMICA ORGANICA | es_ES |
dc.subject.classification | QUIMICA INORGANICA | es_ES |
dc.title | Selective and sensitive colorimetric detection of the neurotransmitter serotonin based on the aggregation of bifunctionalised gold nanoparticles | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.snb.2017.11.181 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//MAT2015-64139-C4-1-R/ES/NANOMATERIALES INTELIGENTES, SONDAS Y DISPOSITIVOS PARA EL DESARROLLO INTEGRADO DE NUEVAS HERRAMIENTAS APLICADAS AL CAMPO BIOMEDICO/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F047/ES/Nuevas aproximaciones para el diseño de materiales de liberación controlada y la detección de compuestos peligrosos/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//MAT2015-64139-C4-4-R/ES/QUIMIOSENSORES CROMOGENICOS Y FLUOROGENICOS PARA LA DETECCION DE NEUROTRASMISORES/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.description.bibliographicCitation | Godoy-Reyes, TM.; Llopis-Lorente, A.; Costero, AM.; Sancenón, F.; Gaviña, P.; Martínez-Máñez, R. (2018). Selective and sensitive colorimetric detection of the neurotransmitter serotonin based on the aggregation of bifunctionalised gold nanoparticles. Sensors and Actuators B Chemical. 258:829-835. https://doi.org/10.1016/j.snb.2017.11.181 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.snb.2017.11.181 | es_ES |
dc.description.upvformatpinicio | 829 | es_ES |
dc.description.upvformatpfin | 835 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 258 | es_ES |
dc.relation.pasarela | S\348852 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |
dc.contributor.funder | Ministerio de Economía, Industria y Competitividad | es_ES |