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dc.contributor.author | Juste-Dolz, Augusto Miguel | es_ES |
dc.contributor.author | Avella-Oliver, Miquel | es_ES |
dc.contributor.author | Puchades, Rosa | es_ES |
dc.contributor.author | Maquieira Catala, Angel | es_ES |
dc.date.accessioned | 2019-02-23T21:06:16Z | |
dc.date.available | 2019-02-23T21:06:16Z | |
dc.date.issued | 2018 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/117235 | |
dc.description.abstract | [EN] Microcontact printing (mu CP) is a practical and versatile approach to create nanostructured patterns of biomolecular probes, but it involves conformational changes on the patterned bioreceptors that often lead to a loss on the biological activity of the resulting structures. Herein we introduce indirect mu CP to create functional patterns of bioreceptors on solid substrates. This is a simple strategy that relies on physisorbing biomolecular probes of interest in the nanostructured gaps that result after patterning backfilling agents by standard mu CP. This study presents the approach, assesses bovine serum albumin as backfilling agent for indirect mu CP on different materials, reports the limitations of standard mu CP on the functionality of patterned antibodies, and demonstrates the capabilities of indirect mu CP to solve this issue. Bioreceptors were herein structured as diffractive gratings and used to measure biorecognition events in label-free conditions. Besides, as a preliminary approach towards sensing biomarkers, this work also reports the implementation of indirect mu CP in an immunoassay to detect human immunoglobulin E. | es_ES |
dc.description.sponsorship | This research was funded by the Spanish Ministry of Economy and Competitiveness (CTQ2016-75749-R), FEDER, Generalitat Valenciana (PROMETEO II/2014/040), and Universitat Politecnica de Valencia (FPI program). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.relation.ispartof | Sensors | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Microcontact printing | es_ES |
dc.subject | Physisorption | es_ES |
dc.subject | Diffraction-based sensing | es_ES |
dc.subject | Label-free | es_ES |
dc.subject | Antibody | es_ES |
dc.subject | Immunoglobulin E | es_ES |
dc.subject | Allergy | es_ES |
dc.subject | Soft lithography | es_ES |
dc.subject | Denaturation | es_ES |
dc.subject | Paratope | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | Indirect Microcontact Printing to Create Functional Patterns of Physisorbed Antibodies | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/s18093163 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CTQ2016-75749-R/ES/BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F040/ES/Estudio de estrategias fisico-químicas para el desarrollo de biosensores interferométricos en soportes interactivos de aplicación en clínica/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.description.bibliographicCitation | Juste-Dolz, AM.; Avella-Oliver, M.; Puchades, R.; Maquieira Catala, A. (2018). Indirect Microcontact Printing to Create Functional Patterns of Physisorbed Antibodies. Sensors. 18(9). https://doi.org/10.3390/s18093163 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://doi.org/10.3390/s18093163 | es_ES |
dc.description.upvformatpinicio | 3163 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 18 | es_ES |
dc.description.issue | 9 | es_ES |
dc.identifier.eissn | 1424-8220 | es_ES |
dc.identifier.pmid | 30235856 | |
dc.identifier.pmcid | PMC6164925 | |
dc.relation.pasarela | S\373890 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |