- -

High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods.

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods.

Show simple item record

Files in this item

dc.contributor.author Santiago Cuellar, Paula es_ES
dc.contributor.author Jiménez Belenguer, Ana Isabel es_ES
dc.contributor.author García Hernández, Jorge es_ES
dc.contributor.author Montes Estellés, Rosa Mª es_ES
dc.contributor.author Hernández Pérez, Manuel es_ES
dc.contributor.author Castillo López, Mª Ángeles es_ES
dc.contributor.author Ferrús Pérez, Mª Antonia es_ES
dc.contributor.author Moreno Trigos, Mª Yolanda es_ES
dc.date.accessioned 2019-03-03T21:07:25Z
dc.date.available 2019-03-03T21:07:25Z
dc.date.issued 2018 es_ES
dc.identifier.issn 1438-4639 es_ES
dc.identifier.uri http://hdl.handle.net/10251/117578
dc.description.abstract [EN] Salmonella spp. is one of the most important causal agents of food-borne illness in developed countries and its presence in irrigation water poses a risk to public health. Its detection in environmental samples is not easy when culture methods are used, and molecular techniques such as PCR or ribosomal rRNA probe hybridization (Fluorescent zn situ Hybridization, FISH) are outstanding alternatives. The aim of this work was to determine the environmental risk due to the presence of Salmonella spp. in wastewater by culture, PCR and FISH. A new specific rDNA probe for Salmonella was designed and its efficiency was compared with the rest of methods Serotype and antibiotic resistance of isolated strains were determined. Forty-five wastewater samples (collected from two secondary wastewater treatment plants) were analysed. Salmonella strains were isolated in 24 wastewater samples (53%), two of them after disinfection treatment. Twenty-three Salmonella strains exhibited resistance to one or more antimicrobial agent. Analysis of wastewater samples yielded PCR positive results for Salmonella in 28 out of the 45 wastewater samples (62%). FISH analysis allowed for the detection of Salmonella in 27 (60%) samples. By using molecular methods, Salmonella was detected in four samples after disinfection treatment. These results show the prevalence of Salmonella in reclaimed wastewater even after U.V. disinfection, what is a matter of public health concern, the high rates of resistance to antibiotics and the adequacy of molecular methods for its rapid detection. FISH method, with SA23 probe developed and assayed in this work provides a tool for detecting Salmonella in water within few hours, with a high rate of effectiveness. es_ES
dc.description.sponsorship Authors gratefully acknowledge the financial support from the Ministerio de Ciencia e Innovacion (Project: AGL2008-05275-C03-02).
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation MECD/AGL2008-05275-C03-02 es_ES
dc.relation.ispartof International Journal of Hygiene and Environmental Health es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject FISH es_ES
dc.subject Molecular detection es_ES
dc.subject Reclaimed water es_ES
dc.subject Salmonella es_ES
dc.subject Wastewater es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.ijheh.2017.10.007 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Santiago Cuellar, P.; Jiménez Belenguer, AI.; García Hernández, J.; Montes Estellés, RM.; Hernández Pérez, M.; Castillo López, MÁ.; Ferrús Pérez, MA.... (2018). High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods. International Journal of Hygiene and Environmental Health. 221(1):95-101. https://doi.org/10.1016/j.ijheh.2017.10.007 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1016/j.ijheh.2017.10.007 es_ES
dc.description.upvformatpinicio 95 es_ES
dc.description.upvformatpfin 101 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 221 es_ES
dc.description.issue 1 es_ES
dc.identifier.pmid 29107574
dc.relation.pasarela 346813 es_ES
dc.contributor.funder Ministerio de Educación es_ES


This item appears in the following Collection(s)

Show simple item record