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A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compounds

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A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compounds

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dc.contributor.author Avella-Oliver, Miquel es_ES
dc.contributor.author Ferrando Martín, Vicente es_ES
dc.contributor.author Monsoriu Serra, Juan Antonio es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Catala, Angel es_ES
dc.date.accessioned 2019-03-09T21:01:54Z
dc.date.available 2019-03-09T21:01:54Z
dc.date.issued 2018 es_ES
dc.identifier.issn 0003-2670 es_ES
dc.identifier.uri http://hdl.handle.net/10251/117898
dc.description.abstract [EN] Herein we present a diffractometric immunosensor to quantify low molecular weight organic compounds in a label-free, simple, and sensitive fashion. The approach is based on patterning analyte analogues (haptens) on solid surfaces according to a diffractive structure, and then loading specific antibodies on them to be subsequently displaced by free analytes in solution. This displacement generates a measurable change in the diffractive response that enables to quantify the analyte concentration. In this study we address the fabrication, optimization, and assessment of these diffractive structures of biological probes and their application to the analysis of atrazine, an organic compound extensively used as pesticide. This immunosensor displays well-correlated dose-response curves that reach a detection limit of 1.1¿ng¿mL¿1 of atrazine in label-free conditions. From a general viewpoint, this study also aims to provide insights into exploiting this approach towards prospective in-field analysis and screening strategies to sense multiple low molecular weight compounds in label-free conditions. es_ES
dc.description.sponsorship This work was supported by the Spanish Ministry of Economy and Competitiveness (CTQ2013-45875-R and FIS2011-23175), FEDER, and the Generalitat Valenciana (PROMETEO II/2014/040 and PROMETEO II/2014/072). Special thanks go to Richard A. McAloney and M. Cynthia Goh for hosting M.A.-O. as visiting researcher, sharing their expertise, and offering their valuable support. M.A.-O. also acknowledges the FPI program of the Spanish Ministry of Economy and Competitiveness for a PhD and an EEBB mobility grant.
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation MINECO/CTQ2013-45875-R es_ES
dc.relation MINECO/FIS2011-23175 es_ES
dc.relation GV/PROMETEOII/2014/040 es_ES
dc.relation GV/PROMETEOII/2014/072 es_ES
dc.relation.ispartof Analytica Chimica Acta es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Biograting es_ES
dc.subject Diffraction-based sensing es_ES
dc.subject Atrazine es_ES
dc.subject Label-free es_ES
dc.subject Microcontact printing es_ES
dc.subject Displacement immunoassay es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.subject.classification MATEMATICA APLICADA es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compounds es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.aca.2018.05.060 es_ES
dc.rights.accessRights Embargado es_ES
dc.date.embargoEndDate 2020-11-29 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation Avella-Oliver, M.; Ferrando Martín, V.; Monsoriu Serra, JA.; Puchades, R.; Maquieira Catala, A. (2018). A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compounds. Analytica Chimica Acta. 1033:173-179. https://doi.org/10.1016/j.aca.2018.05.060 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1016/j.aca.2018.05.060 es_ES
dc.description.upvformatpinicio 173 es_ES
dc.description.upvformatpfin 179 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 1033 es_ES
dc.identifier.pmid 30172323
dc.relation.pasarela 367744 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES


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