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Development and Genetic Characterization of Advanced Backcross Materials and An Introgression Line Population of Solanum incanum in a S. melongena Background

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Development and Genetic Characterization of Advanced Backcross Materials and An Introgression Line Population of Solanum incanum in a S. melongena Background

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dc.contributor.author Gramazio, Pietro es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.contributor.author Plazas Ávila, María de la O es_ES
dc.contributor.author Mangino, Giulio es_ES
dc.contributor.author Herraiz García, Francisco Javier es_ES
dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.date.accessioned 2019-05-04T20:02:18Z
dc.date.available 2019-05-04T20:02:18Z
dc.date.issued 2017 es_ES
dc.identifier.uri http://hdl.handle.net/10251/119861
dc.description.abstract [EN] Advanced backcrosses (ABs) and introgression lines (ILs) of eggplant (Solanum melongena) can speed up genetics and genomics studies and breeding in this crop. We have developed the first full set of ABs and ILs in eggplant using Solanum incanum, a wild eggplant that has a relatively high tolerance to drought, as a donor parent. The development of these ABs and IL eggplant populations had a low efficiency in the early stages, because of the lack of molecular markers and genomic tools. However, this dramatically improved after performing genotyping-by-sequencing in the first round of selfing, followed by high-resolution-melting single nucleotide polymorphism genotyping in subsequent selection steps. A set of 73 selected ABs covered 99% of the S. incanum genome, while 25 fixed immortal ILs, each carrying a single introgressed fragment in homozygosis, altogether spanned 61.7% of the S. incanum genome. The introgressed size fragment in the ILs contained between 0.1 and 10.9% of the S. incanum genome, with a mean value of 4.3%. Sixty-eight candidate genes involved in drought tolerance were identified in the set of ILs. This first set of ABs and ILs of eggplant will be extremely useful for the genetic dissection of traits of interest for eggplant, and represents an elite material for introduction into the breeding pipelines for developing new eggplant cultivars adapted to the challenges posed by the climate-change scenario. es_ES
dc.description.sponsorship This work has been funded in part by the initiative "Adapting Agriculture to Climate Change: Collecting, Protecting and Preparing Crop Wild Relatives," which is supported by the Government of Norway. This project is managed by the Global Crop Diversity Trust with the Millennium Seed Bank of the Royal Botanic Gardens, Kew and implemented in partnership with national and international gene banks and plant breeding institutes around the world. For further information see the project website: http://www.cwrdiversity.org/. Funding has also been received from the European Union's Horizon 2020 Research and Innovation Programme under grant agreement No 677379 (G2P-SOL project: Linking genetic resources, genomes and phenotypes of Solanaceous crops) and from Spanish Ministerio de Economia, Industria y Competitividad and Fondo Europeo de Desarrollo Regional (grant AGL201564755-R from MINECO/ FEDER). PG is grateful to Universitat Politecnica de Valencia for a pre-doctoral (Programa FPI de la UPV-Subprograma 1/2013 call) contract. MP is grateful to Ministerio de Economia, Industria y Competitividad for a post-doctoral grant within the Juan de la Cierva programme (FCJI-2015-24835). GM is grateful to Conselleria d'Educacio, Investigacio, Cultura i Esport de la Generalitat Valenciana for a pre-doctoral grant within the Santiago Grisolia programme (GRISOLIAP/2016/012). Authors also thank the Italian Eggplant Genome Sequencing Consortium for providing access to an improved version of the eggplant genome. es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//AGL2015-64755-R/ES/MEJORA GENETICA DE LA CALIDAD FUNCIONAL Y APARENTE DE LA BERENJENA/ es_ES
dc.relation GV/GRISOLIA/2016/012 es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//FJCI-2015-24835/ES/FJCI-2015-24835/
dc.relation.ispartof Frontiers in Plant Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Solanum melongena es_ES
dc.subject Solanum incanum es_ES
dc.subject Advanced backcrosses es_ES
dc.subject Introgression lines es_ES
dc.subject Drought tolerance es_ES
dc.subject High-throughput es_ES
dc.subject.classification GENETICA es_ES
dc.title Development and Genetic Characterization of Advanced Backcross Materials and An Introgression Line Population of Solanum incanum in a S. melongena Background es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fpls.2017.01477 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/677379/EU/Linking genetic resources, genomes and phenotypes of Solanaceous crops/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Gramazio, P.; Prohens Tomás, J.; Plazas Ávila, MDLO.; Mangino, G.; Herraiz García, FJ.; Vilanova Navarro, S. (2017). Development and Genetic Characterization of Advanced Backcross Materials and An Introgression Line Population of Solanum incanum in a S. melongena Background. Frontiers in Plant Science. 8. https://doi.org/10.3389/fpls.2017.01477 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.3389/fpls.2017.01477 es_ES
dc.description.upvformatpinicio 1477 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.identifier.eissn 1664-462X es_ES
dc.identifier.pmid 28912788
dc.identifier.pmcid PMC5582342
dc.relation.pasarela S\347958 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES


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