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Comparison of European eel sperm cryopreservation protocols with standardization as a target

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Comparison of European eel sperm cryopreservation protocols with standardization as a target

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dc.contributor.author Herranz-Jusdado, Juan Germán es_ES
dc.contributor.author Gallego Albiach, Victor es_ES
dc.contributor.author MORINI, MARINA es_ES
dc.contributor.author Rozenfeld, Christoffer es_ES
dc.contributor.author Pérez Igualada, Luz María es_ES
dc.contributor.author Kása, E. es_ES
dc.contributor.author Kollár, T. es_ES
dc.contributor.author Depincé, A. es_ES
dc.contributor.author Labbé, C. es_ES
dc.contributor.author Horváth, Á. es_ES
dc.contributor.author Asturiano Nemesio, Juan Francisco es_ES
dc.date.accessioned 2019-05-08T20:31:32Z
dc.date.available 2019-05-08T20:31:32Z
dc.date.issued 2019 es_ES
dc.identifier.issn 0044-8486 es_ES
dc.identifier.uri http://hdl.handle.net/10251/120142
dc.description.abstract [EN] The critical situation of the European eel (Anguilla anguilla) has urged the development of sperm cryopreservation protocols for reproduction in captivity and cryobanking. In the last years, two research groups have developed their own protocols in Spain and Hungary with positive results, but difficult to compare. Here, a series of experiments were conducted to test the quality of thawed sperm after using both protocols, determining which of them produce the best results and aiming for standardization. The quality of thawed sperm was assessed by studying the motility and kinetic values of thawed sperm from both cryopreservation protocols using a computer-assisted sperm analysis (CASA-Mot) system. In addition, a viability analysis was performed using flow cytometry to test if the cryoprotectants or the freezing-thawing process led to a reduction in spermatozoa survival. Furthermore, since during cryopreservation the sperm was treated with methylated cryoprotectants (DMSO or methanol) that may induce epigenetic changes in the sperm DNA (cytosine methylation) and could affect the offspring, we conducted a luminometric methylation assay (LUMA) to study the DNA methylation levels induced by both protocols. In this work, all the above-mentioned parameters were analyzed in fresh and frozen-thawed sperm samples. Our results showed that thawed sperm samples from both protocols presented lower sperm motility and velocity, and lower percentage of live cells than those shown in fresh sperm samples. Furthermore, sperm samples from the methanol based protocol showed significantly higher motility, velocity and percentage of live spermatozoa than the same sperm samples treated with the DMSO based protocol. In addition, the DMSO based protocol induced a hypomethylation of sperm DNA compared to fresh samples whereas the methanol based protocol did not alter sperm DNA methylation level. Our results indicate that the methanol based protocol is a more suitable protocol that preserves better the motility and genetic qualities of the European eel sperm. es_ES
dc.description.sponsorship Funded by the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement N 642893 (IMPRESS), including the JGHJ and CR predoctoral contracts, the French CRB Anim project "Investissements d'avenir", ANR-11-INBS-0003, as well as the Hungarian EFOP 3.6.3.-VEKOP 16.-2017-00008 project, and by the Higher Education Institutional Excellence Program (1783-3/2018/FEKUTSRAT) awarded by the Ministry of Human Capacities within the framework of water related researches of Szent Istvan University. MM, EK, TK and AH were granted with Short Term Scientific Missions by the COST Office (COST Action FA1205: AQUAGAMETE). es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation COMISION DE LAS COMUNIDADES EUROPEA/642893//MSCA-ITN-2014-ETN es_ES
dc.relation.ispartof Aquaculture es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Anguilla anguilla es_ES
dc.subject Methanol es_ES
dc.subject DMSO es_ES
dc.subject DNA methylation es_ES
dc.subject Epigenetics es_ES
dc.subject.classification ZOOLOGIA es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Comparison of European eel sperm cryopreservation protocols with standardization as a target es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.aquaculture.2018.09.006 es_ES
dc.rights.accessRights Embargado es_ES
dc.date.embargoEndDate 2021-01-01 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.description.bibliographicCitation Herranz-Jusdado, JG.; Gallego Albiach, V.; Morini, M.; Rozenfeld, C.; Pérez Igualada, LM.; Kása, E.; Kollár, T.... (2019). Comparison of European eel sperm cryopreservation protocols with standardization as a target. Aquaculture. 498:539-544. https://doi.org/10.1016/j.aquaculture.2018.09.006 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.aquaculture.2018.09.006 es_ES
dc.description.upvformatpinicio 539 es_ES
dc.description.upvformatpfin 544 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 498 es_ES
dc.relation.pasarela S\367639 es_ES
dc.contributor.funder European Commission es_ES


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