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Arabidopsis ILITHYIA protein is necessary for proper chloroplast biogenesis and root development independent of eIF2alpha phosphorylation

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Arabidopsis ILITHYIA protein is necessary for proper chloroplast biogenesis and root development independent of eIF2alpha phosphorylation

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dc.contributor.author Faus, I. es_ES
dc.contributor.author Niñoles Rodenes, Regina es_ES
dc.contributor.author Kesari, V. es_ES
dc.contributor.author Llabata, P. es_ES
dc.contributor.author Tam, E. es_ES
dc.contributor.author Nebauer, Sergio G. es_ES
dc.contributor.author Santiago, J. es_ES
dc.contributor.author Hauser, M.T. es_ES
dc.contributor.author Gadea Vacas, José es_ES
dc.date.accessioned 2019-05-31T20:44:56Z
dc.date.available 2019-05-31T20:44:56Z
dc.date.issued 2018 es_ES
dc.identifier.issn 0176-1617 es_ES
dc.identifier.uri http://hdl.handle.net/10251/121382
dc.description.abstract [EN] One of the main mechanisms blocking translation after stress situations is mediated by phosphorylation of the alpha-subunit of the eukaryotic initiation factor 2 (eIF2), performed in Arabidopsis by the protein kinase GCN2 which interacts and is activated by ILITHYIA(ILA). ILA is involved in plant immunity and its mutant lines present phenotypes not shared by the gcn2 mutants. The functional link between these two genes remains elusive in plants. In this study, we show that, although both ILA and GCN2 genes are necessary to mediate eIF2 alpha phosphorylation upon treatments with the aromatic amino acid biosynthesis inhibitor glyphosate, their mutants develop distinct root and chloroplast phenotypes. Electron microscopy experiments reveal that ila mutants, but not gcn2, are affected in chloroplast biogenesis, explaining the macroscopic phenotype previously observed for these mutants. ila3 mutants present a complex transcriptional reprogramming affecting defense responses, photosynthesis and protein folding, among others. Double mutant analyses suggest that ILA has a distinct function which is independent of GCN2 and eIF2 alpha phosphorylation. These results suggest that these two genes may have common but also distinct functions in Arabidopsis. es_ES
dc.description.sponsorship Microarray experiments were done in the Genomics Facility of the IBMCP. MTH was supported by the Austrian Science Found (FWF) projectF03707. This work has been supported by the Spanish Ministry for Science and Education (Plan Nacional 2008-2011). es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Journal of Plant Physiology es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject GCN1 es_ES
dc.subject GCN2 es_ES
dc.subject Arabidopsis es_ES
dc.subject Transcriptomic es_ES
dc.subject EIF2 alpha es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.subject.classification FISIOLOGIA VEGETAL es_ES
dc.title Arabidopsis ILITHYIA protein is necessary for proper chloroplast biogenesis and root development independent of eIF2alpha phosphorylation es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.jplph.2018.04.003 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FWF//F03707/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Producción Vegetal - Departament de Producció Vegetal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Faus, I.; Niñoles Rodenes, R.; Kesari, V.; Llabata, P.; Tam, E.; Nebauer, SG.; Santiago, J.... (2018). Arabidopsis ILITHYIA protein is necessary for proper chloroplast biogenesis and root development independent of eIF2alpha phosphorylation. Journal of Plant Physiology. 224:173-182. https://doi.org/10.1016/j.jplph.2018.04.003 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1016/j.jplph.2018.04.003 es_ES
dc.description.upvformatpinicio 173 es_ES
dc.description.upvformatpfin 182 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 224 es_ES
dc.identifier.pmid 29680783
dc.relation.pasarela S\369140 es_ES
dc.contributor.funder Austrian Science Fund


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