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Molecular characterization of the cucumber (Cucumis sativus L.) accessions held at the COMAV s genebank

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Molecular characterization of the cucumber (Cucumis sativus L.) accessions held at the COMAV s genebank

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dc.contributor.author Valcarcel Germes, José Vicente es_ES
dc.contributor.author Pérez De Castro, Ana María es_ES
dc.contributor.author Díez Niclós, Mª José Teresa De Jesús es_ES
dc.contributor.author Peiró Barber, Rosa Mª es_ES
dc.date.accessioned 2019-06-30T20:02:47Z
dc.date.available 2019-06-30T20:02:47Z
dc.date.issued 2018 es_ES
dc.identifier.issn 1695-971X es_ES
dc.identifier.uri http://hdl.handle.net/10251/122924
dc.description.abstract [EN] The cucumber (Cucumis sativus L.) is an important crop worldwide. In the present study, the molecular genetic diversity of 131 Spanish accessions was analyzed using 23 simple sequence repeat (SSRs). Eighteen of these SSRs were polymorphic; the mean number of alleles, mean observed heterozygosity and mean polymorphic information content were 3.2, 0.065 and 0.229, respectively. Seven SSRs showed a polymorphic information content (PIC) ranging from 0.31 to 0.44, therefore they were reasonably informative. Around 60% of the alleles showed a frequency higher than 0.05, and only one allele in the SSR31399 showed a frequency lower than 0.01. In addition, three accession-specific alleles were found. A high proportion of variation among accessions was obtained. In no case all plants of any accession showed the same genotype and only 18 of 131 Spanish accessions had at least two plants with the same genotype. A cluster analysis did not show any relation with morphological types or geographical area. Therefore, these results demonstrated that molecular diversity of the cucumber did not reflect its phenotypic variability. Finally, this study provided information for the rationalization of the cucumber collection of the COMAV. Morphological traits, origin and molecular data were taken into account to select 47 accessions, six belonging to `French¿ type, 15 to `Long¿ type, and 24 to `Short¿ type. Phenotypic and molecular variability contained in the complete collection was conserved in the selected accessions es_ES
dc.description.sponsorship Partially funded by the Generalitat Valenciana (project GV/2012/080). es_ES
dc.language Inglés es_ES
dc.publisher Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria es_ES
dc.relation.ispartof Spanish Journal of Agricultural Research es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Spanish cucumber landraces es_ES
dc.subject Genebank rationalization es_ES
dc.subject Simple sequence repeat es_ES
dc.subject.classification GENETICA es_ES
dc.title Molecular characterization of the cucumber (Cucumis sativus L.) accessions held at the COMAV s genebank es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.5424/sjar/2018161-12351 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//GV%2F2012%2F080/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-06-10-2408/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Valcarcel Germes, JV.; Pérez De Castro, AM.; Díez Niclós, MJTDJ.; Peiró Barber, RM. (2018). Molecular characterization of the cucumber (Cucumis sativus L.) accessions held at the COMAV s genebank. Spanish Journal of Agricultural Research. 16(1):1-11. https://doi.org/10.5424/sjar/2018161-12351 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.5424/sjar/2018161-12351 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 11 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 16 es_ES
dc.description.issue 1 es_ES
dc.relation.pasarela S\362508 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
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