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Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

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Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

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Cordero-Cucart, MT.; Aragonés, V.; Daros Arnau, JA. (2018). Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli. Journal of Visualized Experiments. (141). https://doi.org/10.3791/58472

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/125104

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Title: Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
Author: Cordero-Cucart, María Teresa Aragonés, V. Daros Arnau, Jose Antonio
UPV Unit: Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Issued date:
Abstract:
[EN] With increasing interest in RNA biology and the use of RNA molecules in sophisticated biotechnological applications, the methods to produce large amounts of recombinant RNAs are limited. Here, we describe a protocol ...[+]
Subjects: Biochemistry , Issue 141 , Recombinant RNA , Circular RNA , Viroid,tRNA ligase , RNA aptamer , Escherichia coli
Copyrigths: Reserva de todos los derechos
Source:
Journal of Visualized Experiments. (eissn: 1940-087X )
DOI: 10.3791/58472
Publisher:
Journal of Visualized Experiments
Publisher version: http://doi.org/10.3791/58472
Project ID:
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83184-R/ES/VIRUS DE PLANTAS: PATOGENOS Y TAMBIEN VECTORES PARA LA PRODUCCION DE PROTEINAS, METABOLITOS, RNAS Y NANOPARTICULAS/
info:eu-repo/grantAgreement/AEI//BIO2017-91865-EXP/ES/VIROIDES CONTRA INSECTOS/
Thanks:
This work was supported by grants BIO2017-83184-R and BIO2017-91865-EXP from the Spanish Ministerio de Ciencia, Innovacion y Universidades (co-financed FEDER funds).
Type: Artículo

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