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DNA assembly standards: Setting the low-level programming code for plant biotechnology

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DNA assembly standards: Setting the low-level programming code for plant biotechnology

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dc.contributor.author Vázquez-Vilar, Marta es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.contributor.author Patron, N es_ES
dc.date.accessioned 2019-09-26T09:30:37Z
dc.date.available 2019-09-26T09:30:37Z
dc.date.issued 2018 es_ES
dc.identifier.issn 0168-9452 es_ES
dc.identifier.uri http://hdl.handle.net/10251/126411
dc.description.abstract [EN] Synthetic Biology is defined as the application of engineering principles to biology. It aims to increase the speed, ease and predictability with which desirable changes and novel traits can be conferred to living cells. The initial steps in this process aim to simplify the encoding of new instructions in DNA by establishing low-level programming languages for biology. Together with advances in the laboratory that allow multiple DNA molecules to be efficiently assembled together into a desired order in a single step, this approach has simplified the design and assembly of multigene constructs and has even facilitated the automated construction of synthetic chromosomes. These advances and technologies are now being applied to plants, for which there are a growing number of software and wetware tools for the design, construction and delivery of DNA molecules and for the engineering of endogenous genes. Here we review the efforts of the past decade that have established synthetic biology workflows and tools for plants and discuss the constraints and bottlenecks of this emerging field. es_ES
dc.description.sponsorship Marta Vazquez-Vilar is funded by Wageningen University & Research. Diego Orzaez is funded by the Spanish Ministry of Economy and Competitiveness [grant number BIO2016-78601-R]. Nicola Patron is funded by the UK Biotechnological and Biological Sciences Research Council (BBSRC) and Engineering and Physics Research Council (EPSRC)Synthetic Biology for Growth programme [OpenPlant Synthetic Biology Research Centre, grant number BB/L0I4130/1], and by the Earlham DNA Foundry [grant number BB/CCG1720/1]. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Plant Science es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Synthetic biology es_ES
dc.subject DNA assembly es_ES
dc.subject Molecular cloning es_ES
dc.subject Laboratory automation es_ES
dc.subject Biotechnology es_ES
dc.subject Genome engineering es_ES
dc.subject CRISPR es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title DNA assembly standards: Setting the low-level programming code for plant biotechnology es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.plantsci.2018.02.024 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2016-78601-R/ES/DISEÑO DE CIRCUITOS GENICOS SINTETICOS Y ORTOGONALES PARA PLANTAS MEDIANTE EL USO DE FACTORES PROGRAMABLES DE UNION A DNA BASADOS EN LA ARQUITECTURA CRISPR-CAS9./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//BB%2FL0I4130%2F1/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//BB%2FCCG1720%2F1/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Vázquez-Vilar, M.; Orzáez Calatayud, DV.; Patron, N. (2018). DNA assembly standards: Setting the low-level programming code for plant biotechnology. Plant Science. 273:33-41. https://doi.org/10.1016/j.plantsci.2018.02.024 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1016/j.plantsci.2018.02.024 es_ES
dc.description.upvformatpinicio 33 es_ES
dc.description.upvformatpfin 41 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 273 es_ES
dc.identifier.pmid 29907307
dc.relation.pasarela S\377554 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder UK Research and Innovation es_ES
dc.contributor.funder Engineering and Physical Sciences Research Council, Reino Unido


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