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Rabbit haemorrhagic disease: experimental study of a recent highly pathogenic GI.2/RHDV2/b strain and evaluation of vaccine efficacy

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Rabbit haemorrhagic disease: experimental study of a recent highly pathogenic GI.2/RHDV2/b strain and evaluation of vaccine efficacy

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dc.contributor.author Le Minor, O. es_ES
dc.contributor.author Boucher, S. es_ES
dc.contributor.author Joudou, L. es_ES
dc.contributor.author Mellet, R. es_ES
dc.contributor.author Sourice, M. es_ES
dc.contributor.author Le Moullec, T. es_ES
dc.contributor.author Nicolier, A. es_ES
dc.contributor.author Beilvert, F. es_ES
dc.contributor.author Sigognault-Flochlay, A. es_ES
dc.date.accessioned 2019-10-02T07:34:08Z
dc.date.available 2019-10-02T07:34:08Z
dc.date.issued 2019-09-30
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/126955
dc.description.abstract [EN] In 2010, a variant of the rabbit haemorrhagic disease virus (RHDV) belonging to a new GI.2 genotype was identified in France and rapidly spread worldwide. Due to antigenic difference, new vaccines including G1.2 strains have been developed to confer adequate protection. An increase in the pathogenicity of the circulating strains was recently reported. The objective of this experimental study was to characterise the infection with a highly pathogenic GI.2/RHDV2/b isolate (2017) and assess the efficacy of Filavac VHD K C+V vaccine (Filavie) against this strain. Four and 10-wk-old specific pathogen-free rabbits were inoculated with a recommended dose of vaccine. After 7 d, controls and vaccinated rabbits were challenged and clinically monitored for 14 d. All animals were necropsied and blood, organs and urine were sampled for quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis. In adult groups, regular nasal and rectal swabbing were performed, and faeces were collected after death to monitor RNA shedding. In control groups, the challenge strain induced acute RHD between 31 and 72 h post-inoculation, with a mortality rate of 100% for kits and 89% for adult rabbits. Except for a shorter mean time to death in kits, similar clinical signs and lesions were observed between age groups. The vaccination significantly prevented all mortality, clinical signs, detection of viral RNA in serum and gross lesions in kits and adult rabbits. In adult groups, we also demonstrated that vaccine significantly protected from detectable RNA shedding via naso-conjunctival and rectal routes. Two weeks after challenge, RNA copies were not detected by PCR in the liver, spleen, lungs, kidneys, faeces and urine of vaccinated adult rabbits. The findings for kits were similar, except that very low levels of RNA were present in the liver and spleen of a few rabbits. These data show that immunisation prevented any significant viral multiplication and/or allowed a rapid clearance. We concluded that, despite the quick evolution of GI.2/RHDV2/b strains, the protection conferred by the vaccine remains adequate. In the context of coexistence of both GI.1 and GI.2 genotypes in some countries, with the circulation of multiples recombinant viruses, the vaccination should be based on the association of strains from both genotypes. es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València
dc.relation.ispartof World Rabbit Science
dc.rights Reserva de todos los derechos es_ES
dc.subject Rabbit haemorrhagic disease (RHD) es_ES
dc.subject GI.2/RHDV2 es_ES
dc.subject Pathogenicity es_ES
dc.subject Vaccine es_ES
dc.subject Efficacy es_ES
dc.subject RT-qPCR es_ES
dc.title Rabbit haemorrhagic disease: experimental study of a recent highly pathogenic GI.2/RHDV2/b strain and evaluation of vaccine efficacy es_ES
dc.type Artículo es_ES
dc.date.updated 2019-10-02T07:10:23Z
dc.identifier.doi 10.4995/wrs.2019.11082
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Le Minor, O.; Boucher, S.; Joudou, L.; Mellet, R.; Sourice, M.; Le Moullec, T.; Nicolier, A.... (2019). Rabbit haemorrhagic disease: experimental study of a recent highly pathogenic GI.2/RHDV2/b strain and evaluation of vaccine efficacy. World Rabbit Science. 27(3):143-156. https://doi.org/10.4995/wrs.2019.11082 es_ES
dc.description.accrualMethod SWORD es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2019.11082 es_ES
dc.description.upvformatpinicio 143 es_ES
dc.description.upvformatpfin 156 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 27
dc.description.issue 3
dc.identifier.eissn 1989-8886
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