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Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants

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Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants

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dc.contributor.author Fadl, Aya Mohamed es_ES
dc.contributor.author Ghallab, Abdel-Raouf Morsy es_ES
dc.contributor.author Abou-Ahmed, Mostafa Mohamed es_ES
dc.date.accessioned 2019-10-02T12:01:35Z
dc.date.available 2019-10-02T12:01:35Z
dc.date.issued 2019-06-28
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/127016
dc.description.abstract [EN] The present study aimed to evaluate the effect of supplementation of INRA-82 semen extender with different cryoprotectants (dimethyl sulphoxide; DMSO vs. dimethyl formamide; DMF) on the quality of white New Zealand rabbit buck spermatozoa. We also investigated the possible association between the synergistic action of DMSO and DMF and their relation with INRA-82 extender composition. Semen was collected and pooled from 8 adult rabbit bucks. Pooled semen samples were diluted 1:1 with INRA-82 extender supplemented with DMSO 8%, DMF 8% or a combination of DMSO 4% and DMF 4%. The diluted semen samples were cryopreserved in 0.25 plastic straws. After thawing, progressive motility, sperm viability, sperm abnormalities, membrane integrity, acrosome status, viability index and DNA integrity were evaluated. The results showed that dilution of rabbit buck semen in INRA-82 supplemented with DMSO and DMF (4% each) before freezing significantly (P<0.05) improved sperm motility (42.00%), percentage of live spermatozoa (45.30%), proportions of spermatozoa with intact acrosome (59.75%) and percentage of spermatozoa with non-fragmented DNA (86.04%), compared to those diluted in INRA-82 supplemented either with DMSO 8% (+9, +10, +5 and +7 percentage points, respectively) or with DMF 8% alone (+18 +18, +12 and +9 percentage points, respectively). In conclusion, dilution of rabbit buck semen before freezing with INRA-82 extender supplemented with a combination of DMSO 4% and DMF 4% improved quality of frozen-thawed New Zealand White rabbit spermatozoa. Furthermore, our results also suggest that supplementation of INRA-82 with DMSO or with DMF alone at higher concentrations deteriorates the sperm quality. es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València
dc.relation.ispartof World Rabbit Science
dc.rights Reserva de todos los derechos es_ES
dc.subject Rabbit buck es_ES
dc.subject Cryopreservation es_ES
dc.subject INRA-82 es_ES
dc.subject DMSO es_ES
dc.subject DMF es_ES
dc.title Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants es_ES
dc.type Artículo es_ES
dc.date.updated 2019-10-02T11:49:51Z
dc.identifier.doi 10.4995/wrs.2019.10892
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Fadl, AM.; Ghallab, AM.; Abou-Ahmed, MM. (2019). Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants. World Rabbit Science. 27(2):77-83. https://doi.org/10.4995/wrs.2019.10892 es_ES
dc.description.accrualMethod SWORD es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2019.10892 es_ES
dc.description.upvformatpinicio 77 es_ES
dc.description.upvformatpfin 83 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 27
dc.description.issue 2
dc.identifier.eissn 1989-8886
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