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Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants

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Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants

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dc.contributor.author Fadl, Aya Mohamed es_ES
dc.contributor.author Ghallab, Abdel-Raouf Morsy es_ES
dc.contributor.author Abou-Ahmed, Mostafa Mohamed es_ES
dc.date.accessioned 2019-10-02T12:01:35Z
dc.date.available 2019-10-02T12:01:35Z
dc.date.issued 2019-06-28
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/127016
dc.description.abstract [EN] The present study aimed to evaluate the effect of supplementation of INRA-82 semen extender with different cryoprotectants (dimethyl sulphoxide; DMSO vs. dimethyl formamide; DMF) on the quality of white New Zealand rabbit buck spermatozoa. We also investigated the possible association between the synergistic action of DMSO and DMF and their relation with INRA-82 extender composition. Semen was collected and pooled from 8 adult rabbit bucks. Pooled semen samples were diluted 1:1 with INRA-82 extender supplemented with DMSO 8%, DMF 8% or a combination of DMSO 4% and DMF 4%. The diluted semen samples were cryopreserved in 0.25 plastic straws. After thawing, progressive motility, sperm viability, sperm abnormalities, membrane integrity, acrosome status, viability index and DNA integrity were evaluated. The results showed that dilution of rabbit buck semen in INRA-82 supplemented with DMSO and DMF (4% each) before freezing significantly (P<0.05) improved sperm motility (42.00%), percentage of live spermatozoa (45.30%), proportions of spermatozoa with intact acrosome (59.75%) and percentage of spermatozoa with non-fragmented DNA (86.04%), compared to those diluted in INRA-82 supplemented either with DMSO 8% (+9, +10, +5 and +7 percentage points, respectively) or with DMF 8% alone (+18 +18, +12 and +9 percentage points, respectively). In conclusion, dilution of rabbit buck semen before freezing with INRA-82 extender supplemented with a combination of DMSO 4% and DMF 4% improved quality of frozen-thawed New Zealand White rabbit spermatozoa. Furthermore, our results also suggest that supplementation of INRA-82 with DMSO or with DMF alone at higher concentrations deteriorates the sperm quality. es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València
dc.relation.ispartof World Rabbit Science
dc.rights Reserva de todos los derechos es_ES
dc.subject Rabbit buck es_ES
dc.subject Cryopreservation es_ES
dc.subject INRA-82 es_ES
dc.subject DMSO es_ES
dc.subject DMF es_ES
dc.title Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants es_ES
dc.type Artículo es_ES
dc.date.updated 2019-10-02T11:49:51Z
dc.identifier.doi 10.4995/wrs.2019.10892
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Fadl, AM.; Ghallab, AM.; Abou-Ahmed, MM. (2019). Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants. World Rabbit Science. 27(2):77-83. https://doi.org/10.4995/wrs.2019.10892 es_ES
dc.description.accrualMethod SWORD es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2019.10892 es_ES
dc.description.upvformatpinicio 77 es_ES
dc.description.upvformatpfin 83 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 27
dc.description.issue 2
dc.identifier.eissn 1989-8886
dc.relation.references Alvarenga M.A., Papa F.O., Landim-Alvarenga F.C., Medeiros A.S. 2005. Amides as cryoprotectants for freezing stallion semen: A review. Anim. Reprod. Sci., 89: 105-113. https://doi.org/10.1016/j.anireprosci.2005.07.001 es_ES
dc.relation.references Ashwood-Smith M.J. 1987. Mechanisms of cryoprotectant action. In: Bowler, K., Fuller, B.J., Temperature and Animal Cells. Biologists, Cambridge, UK., 41: 395-406. es_ES
dc.relation.references Bezerra F.S.B., Castelo T.S., Alves H.M., Oliveira I.R.S., Lima G.L., Peixoto G.C.X., Bezerra A.S.D., Silva A.R. 2011. Objective assessment of the cryoprotective effects of dimethyl formamide for freezing goat semen. Cryobiology, 63: 263-266. https://doi.org/10.1016/j.cryobiol.2011.09.136 es_ES
dc.relation.references Chalah T., Seigneurin F., Blesbois E., Brillard J.P. 1999. In vitro comparison of fowl sperm viability in ejaculates frozen by three different techniques and relationship with subsequent fertility in vivo. Cryobiology, 39: 185-191. https://doi.org/10.1006/cryo.1999.2201 es_ES
dc.relation.references Chan P.J., Corselli J.U., Jacobson J.D., Patton W.C., King A. 1999. Spermac stain analysis of human sperm acrosomes. Fertil. Steril., 72: 124-128. https://doi.org/10.1016/S0015-0282(99)00201-0 es_ES
dc.relation.references Courtens J.L. 1995. La congélation de la semence de lapin. Cuniculture, 123: 101-102. es_ES
dc.relation.references Cristanelli M.J., Amann R.P., Squires E.L., Pickett B.W. 1985. Effects of egg yolk and glycerol level in lactose-EDTA-egg yolk extender and of freezing rate on the motility of frozenthawed stallion spermatozoa. Theriogenology, 23: 25-38. es_ES
dc.relation.references https://doi.org/10.1016/0093-691X(85)90166-9 es_ES
dc.relation.references Curry M.R., Redding B.J., Watson P.F. 1995. Determination of water permeability coefficient and its activation energy for rabbit spermatozoa. Cryobiology, 32: 175-181. https://doi.org/10.1006/cryo.1995.1016 es_ES
dc.relation.references Darin-Bennett, A., White, I.G. 1977. Influence of the cholesterol content of mammalian spermatozoa on susceptibility to cold-shock. Cryobiology. 14: 466-470. https://doi.org/10.1016/0011-2240(77)90008-6 es_ES
dc.relation.references Di Iorio M., Rosato M.P., Iaffaldano N. 2012. Effects of semen frozen with DMA and DMSO as cryoprotectants on rabbit reproductive performances. International PhD Workshop on "Welfare, Biotechnology and Quality of Animal Production", Zielonka, (Poland), 5th-8th September, p.3. es_ES
dc.relation.references Domingo P., Olaciregui M., González N., De Blas I., Gil L. 2018. Effects of seminal plasma and different cryoprotectants on rabbit sperm preservation at 16°C. Exp. Anim., 67: 413-420. https://doi.org/10.1538/expanim.17-0152 es_ES
dc.relation.references Evans G., Maxwell W.C. 1987. Salamons' artificial insemination of sheep and goats (No. Ed. 2). Butterworths. Fadl A.M. 2016. Studies on some factors affecting freezability of stallion spermatozoa. M.V.Sc. Thesis, Cairo Univ. es_ES
dc.relation.references Gao D.Y., Mazur P., Critser J.K. 1997. Fundamental cryobiology of mammalian spermatozoa. In: Karow AM, Critser JK (eds) Reproductive tissue banking. Academic press, San Diego. 263-327. https://doi.org/10.1016/B978-012399770-8/50007-1 es_ES
dc.relation.references Gogol P. 1999. Cryopreservation of plasma-deprived rabbit semen. Ann. Anim. Sci., 26: 85-92. es_ES
dc.relation.references Graham J.K., Mocé E. 2005. Fertility evaluation of frozen/thawed semen. Theriogenology, 64: 492-504. https://doi.org/10.1016/j.theriogenology.2005.05.006 es_ES
dc.relation.references Hanada A., Nagase. 1980. Cryoprotective effects of some amides on rabbit spermatozoa. J.Reprod. Fertil., 60: 247-252. https://doi.org/10.1530/jrf.0.0600247 es_ES
dc.relation.references Hall S.E, Negus C, Johinke D, Bathgate R. 2017. Adjusting cryodiluent composition for improved post-thaw quality of rabbit spermatozoa. PLoS ONE, 12: e0175965. https://doi.org/10.1371/journal.pone.0175965 es_ES
dc.relation.references Holt W.V. 2000. Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenology, 53: 47-58. https://doi.org/10.1016/S0093-691X(99)00239-3 es_ES
dc.relation.references Kashiwazaki N., Okuda Y., Seita Y., Hisamatsu S., Sonoki S., Shino M., Masaoka T., Inomata T. 2006. Comparison of glycerol, lactamide, acetamide and dimethyl sulfoxide as cryoprotectants of Japanese white rabbit spermatozoa. J. Reprod. Dev., 52: 511-516. https://doi.org/10.1262/jrd.18008 es_ES
dc.relation.references Long J.A. 2006. Avian semen cryopreservation: what are the biological challenges? Poult. Sci., 85: 232-236. https://doi.org/10.1093/ps/85.2.232 es_ES
dc.relation.references Medeiros A.S.L., Gomes G.M., Carmo M.T., Papa F.O., Alvarenga M.A. 2002. Cryopreservation of stallion sperm using different amides. Theriogenology, 58: 273-276. es_ES
dc.relation.references https://doi.org/10.1016/S0093-691X(02)00898-1 es_ES
dc.relation.references Mesa A., Henao G. 2012. Effect of cholesterol and dimethylformamide on the cryosurvival of Colombian creole stallion sperm. Revista Medicina Veterinaria Zootecnia Cordoba, 17: 2908-2915. https://doi.org/10.21897/rmvz.260 es_ES
dc.relation.references Milovanov V.K. 1962. Biology of reproduction and artificial insemination of farm animals. Selihozizdat, Moscow. es_ES
dc.relation.references Mocé E., Vicente J.S. 2009. Rabbit sperm cryopreservation: a review. Anim. Reprod. Sci., 110: 1-24. https://doi.org/10.1016/j.anireprosci.2008.08.015 es_ES
dc.relation.references Mota Filho A.C., Teles C.H., Juca R.P., Cardoso J.F., Uchoa D.C., Campello C.C., Silva L.D. 2011. Dimethyl formamide as acryoprotectant for canine semen diluted and frozen in ACP- 106C. Theriogenology, 76: 67-72. https://doi.org/10.1016/j.theriogenology.2011.05.010 es_ES
dc.relation.references Olaciregui, M., Gil, L., Montón, A., Luño, V., Jerez, R.A., Martí, J.I. 2014. Cryopreservation of epididymal stallion sperm. Cryobiology 68: 91-95. https://doi.org/10.1016/j.cryobiol.2013.12.009 es_ES
dc.relation.references Partyka A., Niżański W., Ochota M. 2012. Methods of assessment of cryopreserved semen. In: Katkov II (ed) Current Frontiers in Cryobiology. In Tech Open Access Publisher, Rijeka, Croatia. pp 547-574. https://doi.org/10.5772/33565 es_ES
dc.relation.references Pukazhenthi, B.S., Johnson, A., Guthrie, H.D., Songsasen, N., Padilla, L.R., Wolfe, B.A., Coutinho da Silva, M., Alvarenga, M.A., Wildt, D.E. 2014. Improved sperm cryosurvival in diluents containing amides versus glycerol in the Przewalski's horse (Equus ferus przewalskii). Cryobiology 68: 205-214. https://doi.org/10.1016/j.cryobiol.2014.01.013 es_ES
dc.relation.references Sarıözkan S., Tuncer P.B., Bucak M.N., Ulutaş P.A. 2009. Influence of various antioxidants on microscopicoxidative stress indicators and fertilizing ability of frozenthawed bull semen. Acta Vet. Brno.,78: 463-469. https://doi.org/10.2754/avb200978030463 es_ES
dc.relation.references Squires E.L., Keith S.L., Graham J.K. 2004. Evaluation of alternative cryoprotectants for preserving stallion spermatozoa. Theriogenology, 62: 1056-1065. https://doi.org/10.1016/j.theriogenology.2003.12.024 es_ES
dc.relation.references Vidament M., Ecot P., Noue P., Bourgeois C., Magistrini M., Palmer E. 2000. Centrifugation and addition of glycerol at 22°C instead of 4°C improve post-thaw motility and fertility of stallion spermatozoa. Theriogenology, 54: 907-920. es_ES
dc.relation.references https://doi.org/10.1016/S0093-691X(00)00401-5 es_ES
dc.relation.references Viudes de Castro M.P., Vicente J.S. 1996. A simple method for freezing rabbit semen with successful results on fertility and prolificity. Anim. Reprod. Sci., 44: 195-201. es_ES
dc.relation.references https://doi.org/10.1016/0378-4320(96)01550-3 es_ES
dc.relation.references Watson P.F. 2000. The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci., 60: 481-492. es_ES
dc.relation.references https://doi.org/10.1016/S0378-4320(00)00099-3 es_ES


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