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Enhancing the sensitivity in optical biosensing by striped arrays and frequency-domain analysis

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Enhancing the sensitivity in optical biosensing by striped arrays and frequency-domain analysis

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dc.contributor.author Sancho-Fornes, Gabriel es_ES
dc.contributor.author Avella-Oliver, Miquel es_ES
dc.contributor.author Carrascosa Rubio, Javier es_ES
dc.contributor.author Morais, Sergi es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Catala, Angel es_ES
dc.date.accessioned 2020-02-27T21:01:50Z
dc.date.available 2020-02-27T21:01:50Z
dc.date.issued 2019 es_ES
dc.identifier.issn 0925-4005 es_ES
dc.identifier.uri http://hdl.handle.net/10251/137956
dc.description.abstract [EN] An approach to enhance the sensitivity of optical biosensors is developed. It is based on patterning strips of biochemical probes and signal processing (frequency-domain analysis, FDA) to the resulting analytical data after a biorecognition assay. This paper introduces FDA, demonstrates for the first time its potential in real bioanalytical systems, describes its critical parameters, and discusses how to optimize them to efficiently exploit this approach for optical biosensing. The results show that FDA enables for the selective identification and removal of the undesired noise contributions from the analytical signal arising from a biorecognition event of interest, thus increasing signal-to-noise ratios and, therefore, enhancing the sensitivity of the analysis. In this study, we proof the concept with a model immunassay system for IgGs detection (BSA/anti-BSA) that achieves a sensitivity enhancement of up to 3 orders of magnitude, and then FDA is also demonstrated in a sandwich immunoassay to quantify casein that reaches a limit of detection of 4 ng/mL (174 pM). Beyond these particular assays, this work also addres to stablish the basis of this approach and to provide keys to stimulate future developments that expand the potential of FDA to other biorecognition assays, transduction systems, and applications. es_ES
dc.description.sponsorship This research was supported by FEDER and the Spanish Ministry of Economy and Competitiveness (CTQ2016-75749-R). es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Sensors and Actuators B Chemical es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Optical biosensor es_ES
dc.subject Frequency-domain analysis es_ES
dc.subject Sensitivity enhancement es_ES
dc.subject Immunoassay es_ES
dc.subject Casein es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Enhancing the sensitivity in optical biosensing by striped arrays and frequency-domain analysis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.snb.2018.10.130 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CTQ2016-75749-R/ES/BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Sancho-Fornes, G.; Avella-Oliver, M.; Carrascosa Rubio, J.; Morais, S.; Puchades, R.; Maquieira Catala, A. (2019). Enhancing the sensitivity in optical biosensing by striped arrays and frequency-domain analysis. Sensors and Actuators B Chemical. (281):432-438. https://doi.org/10.1016/j.snb.2018.10.130 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.snb.2018.10.130 es_ES
dc.description.upvformatpinicio 432 es_ES
dc.description.upvformatpfin 438 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.issue 281 es_ES
dc.relation.pasarela S\372025 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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