dc.contributor.author |
Calvo-Lerma, Joaquim
|
es_ES |
dc.contributor.author |
Fornes-Ferrer, Victor
|
es_ES |
dc.contributor.author |
Peinado Pardo, Irene
|
es_ES |
dc.contributor.author |
Heredia Gutiérrez, Ana Belén
|
es_ES |
dc.contributor.author |
Ribes-Koninckx C.
|
es_ES |
dc.contributor.author |
Andrés Grau, Ana María
|
es_ES |
dc.date.accessioned |
2020-04-06T08:55:49Z |
|
dc.date.available |
2020-04-06T08:55:49Z |
|
dc.date.issued |
2019-02-22 |
es_ES |
dc.identifier.issn |
1932-6203 |
es_ES |
dc.identifier.uri |
http://hdl.handle.net/10251/140190 |
|
dc.description.abstract |
[EN] Background
Patients with cystic fibrosis have to take enzymatic supplements to allow for food digestion. However, an evidence-based method to adjust Pancreatic Enzyme Replacement Therapy (PERT) is inexistent, and lipid content of meals is used as a rough criterion.
Objective
In this study, an in vitro digestion model was set up to determine the theoretical optimal dose (TOD) of enzymatic supplement for a selection of foods, which is the dose that allows for maximum lipolysis extent.
Methods
A static in vitro digestion model was applied to simulate digestion of eight foods covering a wide range of lipid contents. First, the dose of the enzymatic supplement was fixed at 2000 lipase units per gram of fat (LU/g fat) using intestinal pH and bile salt concentration as variables. Second, intestinal pH and bile salt concentrations were fixed and the variable was the dose of the enzymatic supplement. Lipolysis extent was determined by measuring the free fatty acids released from initial triglycerides content of foods after digestion. Results in terms of percentage of lipolysis extent were fitted into a linear-mixed segmented model and the deducted equations were used to predict the TOD to reach 90% of lipolysis in every food. In addition, the effect of intestinal pH and bile salt concentration were investigated.
Results
The predictive equations obtained for the assessed foods showed that lipolysis was not only dependent on the dose of the enzyme supplement or the lipid content. Moreover, intestinal pH and bile salt concentration had significant effects on lipolysis. Therefore an evidence-based model can be developed taking into account these variables.
Conclusions
Depending on food characteristics, a specific TOD should be assigned to achieve an optimal digestion extent. This work represents a first step towards an evidence-based method for PERT dosing, which will be applied in an in vivo setting to validate its efficacy. |
es_ES |
dc.description.sponsorship |
This work was fully funded by the European Union and the Horizon 2020 Research and Innovation Framework Programme (PHC-26-2014 call Self management of health and disease: citizen engagement and mHealth) under grant number 643806. |
es_ES |
dc.language |
Inglés |
es_ES |
dc.publisher |
Public Library of Science |
es_ES |
dc.relation.ispartof |
PLoS ONE |
es_ES |
dc.rights |
Reconocimiento (by) |
es_ES |
dc.subject.classification |
TECNOLOGIA DE ALIMENTOS |
es_ES |
dc.title |
A first approach for an evidence-based in vitro digestion method to adjust pancreatic enzyme replacement therapy in cystic fibrosis |
es_ES |
dc.type |
Artículo |
es_ES |
dc.identifier.doi |
10.1371/journal.pone.0212459 |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/EC/H2020/643806/EU/Innovative approach for self-management and social welfare of Cystic Fibrosis patients in Europe: development, validation and implementation of a telematics tool./ |
es_ES |
dc.rights.accessRights |
Abierto |
es_ES |
dc.contributor.affiliation |
Universitat Politècnica de València. Departamento de Tecnología de Alimentos - Departament de Tecnologia d'Aliments |
es_ES |
dc.contributor.affiliation |
Universitat Politècnica de València. Instituto Universitario de Ingeniería de Alimentos para el Desarrollo - Institut Universitari d'Enginyeria d'Aliments per al Desenvolupament |
es_ES |
dc.description.bibliographicCitation |
Calvo-Lerma, J.; Fornes-Ferrer, V.; Peinado Pardo, I.; Heredia Gutiérrez, AB.; Ribes-Koninckx C.; Andrés Grau, AM. (2019). A first approach for an evidence-based in vitro digestion method to adjust pancreatic enzyme replacement therapy in cystic fibrosis. PLoS ONE. 14(2):1-14. https://doi.org/10.1371/journal.pone.0212459 |
es_ES |
dc.description.accrualMethod |
S |
es_ES |
dc.relation.publisherversion |
https://doi.org/10.1371/journal.pone.0212459 |
es_ES |
dc.description.upvformatpinicio |
1 |
es_ES |
dc.description.upvformatpfin |
14 |
es_ES |
dc.type.version |
info:eu-repo/semantics/publishedVersion |
es_ES |
dc.description.volume |
14 |
es_ES |
dc.description.issue |
2 |
es_ES |
dc.relation.pasarela |
S\379077 |
es_ES |
dc.description.references |
Lesmes, U., & McClements, D. J. (2012). Controlling lipid digestibility: Response of lipid droplets coated by β-lactoglobulin-dextran Maillard conjugates to simulated gastrointestinal conditions. Food Hydrocolloids, 26(1), 221-230. doi:10.1016/j.foodhyd.2011.05.011 |
es_ES |
dc.description.references |
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es_ES |
dc.description.references |
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es_ES |
dc.description.references |
Muggeo, V. & Muggeo, V. M. R. Segmented mixed models with random changepoints in R Working paper (2016). |
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