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Live Tracking Biofunctionalization and Label-Free Protein Detection Performed by a Nanophotonic Biosensor

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Live Tracking Biofunctionalization and Label-Free Protein Detection Performed by a Nanophotonic Biosensor

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dc.contributor.author Sabek, Jad es_ES
dc.contributor.author Torrijos-Morán, Luis es_ES
dc.contributor.author Díaz-Betancor, Zeneida es_ES
dc.contributor.author Bañuls Polo, María-José es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.contributor.author García-Rupérez, Jaime es_ES
dc.date.accessioned 2020-04-06T08:56:43Z
dc.date.available 2020-04-06T08:56:43Z
dc.date.issued 2018-11-14 es_ES
dc.identifier.uri http://hdl.handle.net/10251/140221
dc.description.abstract [EN] A label-free biosensor based on silicon-on-insulator (SOI) photonic bandgap (PBG) structures is performed for specific protein detection. First, the SOI sensing surface is functionalized using triethoxyvinylsilane (TEVS) organosilane. Then, a UV light photocatalyzed immobilization of polyclonal half anti-bovine serum albumin (haBSA) antibodies is performed. Finally, a direct detection of target BSA antigen is carried out. Both the immobilization and the detection steps are monitored by making a continuous tracking of the PBG edge shift. In order to confirm the recognition of the antigen by the immobilized antibody, a fluorophore-labelled secondary antibody was flowed at the end of the experiment in order to perform a confirmation fluorescence test after the photonic detection. es_ES
dc.description.sponsorship This work was supported by Horizon 2020 Framework Program of the European Union under the project H2020-PHC-634013 (PHOCNOSIS). es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Proceedings es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Photonic sensor es_ES
dc.subject Photonic bandgap es_ES
dc.subject Silicon on insulator es_ES
dc.subject Biofunctionalization es_ES
dc.subject Photocatalysis es_ES
dc.subject Half antibodies es_ES
dc.subject.classification TEORIA DE LA SEÑAL Y COMUNICACIONES es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Live Tracking Biofunctionalization and Label-Free Protein Detection Performed by a Nanophotonic Biosensor es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/ecsa-5-05718 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/634013/EU/Advanced nanophotonic point-of-care analysis device for fast and early diagnosis of cardiovascular diseases/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Tecnología Nanofotónica - Institut Universitari de Tecnologia Nanofotònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Comunicaciones - Departament de Comunicacions es_ES
dc.description.bibliographicCitation Sabek, J.; Torrijos-Morán, L.; Díaz-Betancor, Z.; Bañuls Polo, M.; Maquieira Catala, Á.; García-Rupérez, J. (2018). Live Tracking Biofunctionalization and Label-Free Protein Detection Performed by a Nanophotonic Biosensor. Proceedings. 4(1):1-6. https://doi.org/10.3390/ecsa-5-05718 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/ecsa-5-05718 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 6 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 4 es_ES
dc.description.issue 1 es_ES
dc.identifier.eissn 2504-3900 es_ES
dc.relation.pasarela S\376748 es_ES
dc.contributor.funder European Commission es_ES


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