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dc.contributor.author | Gugutkov, Dencho | es_ES |
dc.contributor.author | Awaja, Firas | es_ES |
dc.contributor.author | Belemezova, Kalina | es_ES |
dc.contributor.author | Keremidarska, Milena | es_ES |
dc.contributor.author | Krasteva, Natalia | es_ES |
dc.contributor.author | Kyurkchiev, Stanimir | es_ES |
dc.contributor.author | Gallego-Ferrer, Gloria | es_ES |
dc.contributor.author | Seker, Sukran | es_ES |
dc.contributor.author | Elcin, Ayse Eser | es_ES |
dc.contributor.author | Elçin, Yasar Murat | es_ES |
dc.contributor.author | Altankov, George | es_ES |
dc.date.accessioned | 2020-04-17T12:51:05Z | |
dc.date.available | 2020-04-17T12:51:05Z | |
dc.date.issued | 2017 | es_ES |
dc.identifier.issn | 1549-3296 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/140939 | |
dc.description.abstract | [EN] Novel, hybrid fibrinogen/polylactic acid (FBG/PLA) nanofibers with different configuration (random vs aligned) and dimensionality (2¿D vs 3¿D environment) were used to control the overall behavior and the osteogenic differentiation of human adipose¿derived mesenchymal stem cells (ADMSCs). Aligned nanofibers in both the 2¿D and 3¿D configurations are proved to be favored for osteodifferentiation. Morphologically, we found that on randomly configured nanofibers, the cells developed a stellate¿like morphology with multiple projections; however, time¿lapse analysis showed significantly diminished cell movements. Conversely, an elongated cell shape with advanced cell spreading and extended actin cytoskeleton accompanied with significantly increased cell mobility were observed when cells attached on aligned nanofibers. Moreover, a clear tendency for higher alkaline phosphatase activity was also found on aligned fibers when ADMSCs were switched to osteogenic induction medium. The strongest accumulation of Alizarin red (AR) and von Kossa stain at 21 days of culture in osteogenic medium were found on 3¿D aligned constructs while the rest showed lower and rather undistinguishable activity. Quantitative reverse transcription¿polymerase chain reaction analysis for Osteopontin (OSP) and RUNX 2 generally confirmed this trend showing favorable expression of osteogenic genes activity in 3¿D environment particularly in aligned configuration. | es_ES |
dc.description.sponsorship | Contract grant sponsor: CIBER-BBN Spain (project BIOSURFACES) Contract grant sponsor: European Commission through the FP7 Industry-Academia Partnerships and Pathways (IAPP) project FIBROGELNET; contract grant number: PAP-GA-2012-324386 Contract grant sponsor: Spanish Ministry of Economy and Competitiveness; contract grant number: MAT 2015-69315-C3 MYOHEAL Contract grant sponsor: The Scientific and Technological Research Council of Turkey (TUBITAK); contract grant number: 11S497 | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | John Wiley & Sons | es_ES |
dc.relation.ispartof | Journal of Biomedical Materials Research Part A | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Mesenchymal stem cells | es_ES |
dc.subject | Nanofibers | es_ES |
dc.subject | Osteogenic | es_ES |
dc.subject | Fibrinogen | es_ES |
dc.subject | Cell movements | es_ES |
dc.subject.classification | MAQUINAS Y MOTORES TERMICOS | es_ES |
dc.title | Osteogenic differentiation of mesenchymal stem cells using hybrid nanofibers with different configurations and dimensionality | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1002/jbm.a.36065 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/324386/EU/Network for Development of Soft Nanofibrous Construct for Cellular Therapy of Degenerative Skeletal Disorders/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//MAT2015-69315-C3-2-R/ES/REMODELACION POR MIOBLASTOS DE LA MATRIZ EXTRACELULAR EN LA INTERFAZ CELULA-BIOMATERIAL/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/TUBITAK//11S497/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//MAT2015-69315-C3-1-R/ES/SOPORTES CELULARES BIODEGRADABLES CARGADOS CON IONES BIOACTIVOS PARA REGENERACION MUSCULAR/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada | es_ES |
dc.description.bibliographicCitation | Gugutkov, D.; Awaja, F.; Belemezova, K.; Keremidarska, M.; Krasteva, N.; Kyurkchiev, S.; Gallego-Ferrer, G.... (2017). Osteogenic differentiation of mesenchymal stem cells using hybrid nanofibers with different configurations and dimensionality. Journal of Biomedical Materials Research Part A. 105(7):2065-2074. https://doi.org/10.1002/jbm.a.36065 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1002/jbm.a.36065 | es_ES |
dc.description.upvformatpinicio | 2065 | es_ES |
dc.description.upvformatpfin | 2074 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 105 | es_ES |
dc.description.issue | 7 | es_ES |
dc.relation.pasarela | S\357246 | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |
dc.contributor.funder | Technological Research Council of Turkey | es_ES |
dc.contributor.funder | Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina | es_ES |
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