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Osteogenic differentiation of mesenchymal stem cells using hybrid nanofibers with different configurations and dimensionality

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Osteogenic differentiation of mesenchymal stem cells using hybrid nanofibers with different configurations and dimensionality

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Nombre: Gugutkov;Awaja;Be ...
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dc.contributor.author Gugutkov, Dencho es_ES
dc.contributor.author Awaja, Firas es_ES
dc.contributor.author Belemezova, Kalina es_ES
dc.contributor.author Keremidarska, Milena es_ES
dc.contributor.author Krasteva, Natalia es_ES
dc.contributor.author Kyurkchiev, Stanimir es_ES
dc.contributor.author Gallego-Ferrer, Gloria es_ES
dc.contributor.author Seker, Sukran es_ES
dc.contributor.author Elcin, Ayse Eser es_ES
dc.contributor.author Elçin, Yasar Murat es_ES
dc.contributor.author Altankov, George es_ES
dc.date.accessioned 2020-04-17T12:51:05Z
dc.date.available 2020-04-17T12:51:05Z
dc.date.issued 2017 es_ES
dc.identifier.issn 1549-3296 es_ES
dc.identifier.uri http://hdl.handle.net/10251/140939
dc.description.abstract [EN] Novel, hybrid fibrinogen/polylactic acid (FBG/PLA) nanofibers with different configuration (random vs aligned) and dimensionality (2¿D vs 3¿D environment) were used to control the overall behavior and the osteogenic differentiation of human adipose¿derived mesenchymal stem cells (ADMSCs). Aligned nanofibers in both the 2¿D and 3¿D configurations are proved to be favored for osteodifferentiation. Morphologically, we found that on randomly configured nanofibers, the cells developed a stellate¿like morphology with multiple projections; however, time¿lapse analysis showed significantly diminished cell movements. Conversely, an elongated cell shape with advanced cell spreading and extended actin cytoskeleton accompanied with significantly increased cell mobility were observed when cells attached on aligned nanofibers. Moreover, a clear tendency for higher alkaline phosphatase activity was also found on aligned fibers when ADMSCs were switched to osteogenic induction medium. The strongest accumulation of Alizarin red (AR) and von Kossa stain at 21 days of culture in osteogenic medium were found on 3¿D aligned constructs while the rest showed lower and rather undistinguishable activity. Quantitative reverse transcription¿polymerase chain reaction analysis for Osteopontin (OSP) and RUNX 2 generally confirmed this trend showing favorable expression of osteogenic genes activity in 3¿D environment particularly in aligned configuration. es_ES
dc.description.sponsorship Contract grant sponsor: CIBER-BBN Spain (project BIOSURFACES) Contract grant sponsor: European Commission through the FP7 Industry-Academia Partnerships and Pathways (IAPP) project FIBROGELNET; contract grant number: PAP-GA-2012-324386 Contract grant sponsor: Spanish Ministry of Economy and Competitiveness; contract grant number: MAT 2015-69315-C3 MYOHEAL Contract grant sponsor: The Scientific and Technological Research Council of Turkey (TUBITAK); contract grant number: 11S497 es_ES
dc.language Inglés es_ES
dc.publisher John Wiley & Sons es_ES
dc.relation.ispartof Journal of Biomedical Materials Research Part A es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Mesenchymal stem cells es_ES
dc.subject Nanofibers es_ES
dc.subject Osteogenic es_ES
dc.subject Fibrinogen es_ES
dc.subject Cell movements es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Osteogenic differentiation of mesenchymal stem cells using hybrid nanofibers with different configurations and dimensionality es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1002/jbm.a.36065 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/324386/EU/Network for Development of Soft Nanofibrous Construct for Cellular Therapy of Degenerative Skeletal Disorders/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2015-69315-C3-2-R/ES/REMODELACION POR MIOBLASTOS DE LA MATRIZ EXTRACELULAR EN LA INTERFAZ CELULA-BIOMATERIAL/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/TUBITAK//11S497/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2015-69315-C3-1-R/ES/SOPORTES CELULARES BIODEGRADABLES CARGADOS CON IONES BIOACTIVOS PARA REGENERACION MUSCULAR/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Gugutkov, D.; Awaja, F.; Belemezova, K.; Keremidarska, M.; Krasteva, N.; Kyurkchiev, S.; Gallego-Ferrer, G.... (2017). Osteogenic differentiation of mesenchymal stem cells using hybrid nanofibers with different configurations and dimensionality. Journal of Biomedical Materials Research Part A. 105(7):2065-2074. https://doi.org/10.1002/jbm.a.36065 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1002/jbm.a.36065 es_ES
dc.description.upvformatpinicio 2065 es_ES
dc.description.upvformatpfin 2074 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 105 es_ES
dc.description.issue 7 es_ES
dc.relation.pasarela S\357246 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Technological Research Council of Turkey es_ES
dc.contributor.funder Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina es_ES
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