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Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation

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Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation

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dc.contributor.author Tortajada-Genaro, Luis Antonio es_ES
dc.contributor.author Yamanaka, Eric Seiti es_ES
dc.contributor.author Maquieira Catala, Angel es_ES
dc.date.accessioned 2020-04-24T07:14:03Z
dc.date.available 2020-04-24T07:14:03Z
dc.date.issued 2019 es_ES
dc.identifier.issn 0039-9140 es_ES
dc.identifier.uri http://hdl.handle.net/10251/141451
dc.description.abstract [EN] Consumer electronic technologies offer practical performances to develop compact biosensing systems intended for the point-of-care testing of DNA biomarkers. Herein a discrimination method for detecting single nucleotide polymorphisms, based on isothermal amplification and on-chip hybridisation, was developed and integrated into user-friendly optical devices: e.g., USB digital microscope, flatbed scanner, smartphone and DVD drive. In order to adequately identify a single base change, loop-mediated isothermal amplification (LAMP) was employed, with high yields (8 orders) within 45 min. Subsequently, products were directly hybridised to the allele-specific probes attached to plastic chips in an array format. After colorimetric staining, four consumer electronic techniques were compared. Sensitive precise measurements were taken (high signal-to-noise ratios, 10-mu m image resolution, 99% scan-to-scan reproducibility). These features confirmed their potential as analytical tools, are a competitive alternative to fluorescence scanners, and incorporate additional advantages, such as user-friendly interface and connectivity for telemedicine needs. The analytical performances of the integrated platform (assay and reader) in the human samples were also excellent, with a low detection limit (100 genomic DNA copies), and reproducible (< 15%) and cheap assays (< 10 (sic)/test). The correct genotyping of a genetic biomarker (single-nucleotide polymorphism located in the GRIK4 gene) was achieved as the assigned genotypes agreed with those determined by using sequencing. The portability, favourable discriminating and read-out capabilities reveal that the implementation of mass-produced low-cost devices into minimal-specialised clinical laboratories is closer to becoming a reality. es_ES
dc.description.sponsorship The authors acknowledge the financial support received from the Generalitat Valenciana, Spain (GVA-PROMETEOII/2014/040 Project and GRISOLIA/2014/024 Ph.D. grant) and the Spanish Ministry of Economy and Competitiveness, Spain (MINECO CTQ2016-75749-R project) by U. E. FEDER funds. The authors also thank J. Carrascosa for supporting the DVD reader measurements. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation GV/GRISOLIA/2014/024 es_ES
dc.relation GENERALITAT VALENCIANA/PROMETEOII/2014/040 es_ES
dc.relation AEI/CTQ2016-75749-R es_ES
dc.relation.ispartof Talanta es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Single-nucleotide polymorphism es_ES
dc.subject Isothermal DNA amplification es_ES
dc.subject Point-of-care testing es_ES
dc.subject Smartphone es_ES
dc.subject Scanner es_ES
dc.subject Compact disc es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.talanta.2019.01.124 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Tortajada-Genaro, LA.; Yamanaka, ES.; Maquieira Catala, A. (2019). Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation. Talanta. 198:424-431. https://doi.org/10.1016/j.talanta.2019.01.124 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.talanta.2019.01.124 es_ES
dc.description.upvformatpinicio 424 es_ES
dc.description.upvformatpfin 431 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 198 es_ES
dc.relation.pasarela S\388821 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES


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