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Rabbit seminal plasma proteome: The importance of the genetic origin.

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Rabbit seminal plasma proteome: The importance of the genetic origin.

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dc.contributor.author Casares-Crespo, Lucía es_ES
dc.contributor.author Fernández-Serrano, P. es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.contributor.author Marco-Jiménez, Francisco es_ES
dc.contributor.author Viudes de Castro, María Pilar es_ES
dc.date.accessioned 2020-04-27T05:55:18Z
dc.date.available 2020-04-27T05:55:18Z
dc.date.issued 2018 es_ES
dc.identifier.issn 0378-4320 es_ES
dc.identifier.uri http://hdl.handle.net/10251/141517
dc.description.abstract [EN] The present study was conducted to characterise rabbit seminal plasma proteins (SP proteins) focusing on the influence of the genetic origin and seasonality. In addition, ß-NGF protein quantity in SP was determined. Semen samples were recovered from January to December 2014 using 6 males belonging to genotype A and six from genotype R. For each genotype, one pooled sample at the beginning, middle and end of each season was selected to develop the experiment. A total of 24 pools (3 for each season and genetic line) were analysed. SP proteins of the two experimental groups were recovered and subjected to in-solution digestion nano LC¿MS/MS and bioinformatics analysis. The resulting library included 402 identified proteins validated with ¿95% Confidence (unused Score¿1.3). These data are available via ProteomeXchange with identifier PXD006308. Only 6 proteins were specifically implicated in reproductive processes according to Gene Ontology annotation. Twenty-three proteins were differentially expressed between genotypes, 11 over-expressed in genotype A and 12 in genotype R. Regarding the effect of season on rabbit SP proteome, results showed that there is no clear pattern of protein variation throughout the year. Similar ß-NGF relative quantity was observed between seasons and genotypes. In conclusion, this study generates the largest library of SP proteins reported to date in rabbits and provides evidence that genotype is related to a specific abundance of SP proteins. es_ES
dc.description.sponsorship This research was supported in part by the RTA2013-00058-00-00 from INIA, the European Social Fund and the European FEDER Funds. L. Casares-Crespo is supported by a scholarship from Instituto Valenciano de Investigaciones Agrarias (IVIA) and the European Social Fund. P. Fernandez-Serrano is supported by Spanish funds from IVIA and Ministerio de Empleo y Seguridad Social (Youth Guarantee Program). The authors are grateful to M. Luz Valero for her excellent technical assistance. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation INIA/RTA2013-00058-00-00 es_ES
dc.relation.ispartof Animal Reproduction Science es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Rabbit es_ES
dc.subject Seminal plasma es_ES
dc.subject Proteome es_ES
dc.subject Genotype es_ES
dc.subject Season es_ES
dc.subject LC MS/MS es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Rabbit seminal plasma proteome: The importance of the genetic origin. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.anireprosci.2017.12.004 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Casares-Crespo, L.; Fernández-Serrano, P.; Vicente Antón, JS.; Marco-Jiménez, F.; Viudes De Castro, MP. (2018). Rabbit seminal plasma proteome: The importance of the genetic origin. Animal Reproduction Science. 189:30-42. https://doi.org/10.1016/j.anireprosci.2017.12.004 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.anireprosci.2017.12.004 es_ES
dc.description.upvformatpinicio 30 es_ES
dc.description.upvformatpfin 42 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 189 es_ES
dc.relation.pasarela S\349070 es_ES
dc.contributor.funder European Social Fund es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Empleo y Seguridad Social es_ES
dc.contributor.funder Institut Valencià d'Investigacions Agràries es_ES
dc.contributor.funder Instituto Nacional de Investigaciones Agrarias es_ES


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