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DVC-FISH to identify potentially pathogenic Legionella inside free-living amoebae from water sources

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DVC-FISH to identify potentially pathogenic Legionella inside free-living amoebae from water sources

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dc.contributor.author Moreno Trigos, Mª Yolanda es_ES
dc.contributor.author Moreno-Mesonero, Laura es_ES
dc.contributor.author García Hernández, Jorge es_ES
dc.date.accessioned 2020-04-29T07:04:32Z
dc.date.available 2020-04-29T07:04:32Z
dc.date.issued 2019-09 es_ES
dc.identifier.issn 0013-9351 es_ES
dc.identifier.uri http://hdl.handle.net/10251/141950
dc.description.abstract [EN] Despite all safety efforts, drinking and wastewater can still be contaminated by Legionella and free-living amoebae (FLA) since these microorganisms are capable of resisting disinfection treatments. An amoebae cyst harboring pathogenic Legionella spp. can be a transporter of this organism, protecting it and enhancing its infection abilities. Therefore, the aim of this work is to identify by DVC-FISH viable Legionella spp and Legionella pneumophila cells inside FLA from water sources in a specific and rapid way with the aim of assessing the real risk of these waters. A total of 55 water samples were processed, 30 reclaimed wastewater and 25 drinking water. FLA presence was detected in 52.7% of the total processed water samples. When DVC-FISH technique was applied, the presence of viable internalized Legionella spp. cells was identified in 69.0% of the total FLA-positive samples, concretely in 70.0% and 66.7% of wastewater and drinking water samples, respectively. L. pneumophila was simultaneously identified in 48.3% of the total FLA-positive samples, specifically in 50.0% and 44.4% of wastewater and drinking water samples, respectively. By culture, potentially pathogenic Legionella cells were recovered in 27.6% of the total FLA-positive bacteria, particularly in 35.0% and 11.1% of wastewater and drinking water samples, respectively. These findings demonstrate that FLA may promote resistance of bacteria to the performed disinfection treatments for drinking as well as for wastewater. So, in addition to the risk for the presence of pathogenic FLA in water it is necessary to take into account that these can be transporters of the pathogenic bacteria Legionella, which are able to survive inside them. The DVC-FISH method described here has been proved to be a rapid and specific tool to identify pathogenic Legionella spp. and L. penumophila viable cells harboured by FLA in these water sources, posing particular public health concern. es_ES
dc.description.sponsorship This study has been supported by First research projects funding (PAID-06-18), Vice-Rectorate for Research, Innovation and Transfer of Universitat Politècnica de València" (UPV), València, Spain. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Environmental Research es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Free-living amoebae es_ES
dc.subject Legionella spp. es_ES
dc.subject DVC-FISH es_ES
dc.subject Wastewater es_ES
dc.subject Drinking water es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title DVC-FISH to identify potentially pathogenic Legionella inside free-living amoebae from water sources es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.envres.2019.06.002 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-06-18/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Moreno Trigos, MY.; Moreno-Mesonero, L.; García Hernández, J. (2019). DVC-FISH to identify potentially pathogenic Legionella inside free-living amoebae from water sources. Environmental Research. 176:1-7. https://doi.org/10.1016/j.envres.2019.06.002 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.envres.2019.06.002 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 7 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 176 es_ES
dc.identifier.pmid 31195295 es_ES
dc.relation.pasarela S\394449 es_ES
dc.contributor.funder Universitat Politècnica de València es_ES


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