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Association of Melanophilin (MLPH) gene polymorphism with coat colour in Rex rabbits

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Association of Melanophilin (MLPH) gene polymorphism with coat colour in Rex rabbits

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dc.contributor.author Li, J. es_ES
dc.contributor.author Chen, Y. es_ES
dc.contributor.author Liu, M. es_ES
dc.contributor.author Chen, Q. es_ES
dc.contributor.author Zhou, J. es_ES
dc.contributor.author Bao, G. es_ES
dc.contributor.author Wu, X. es_ES
dc.date.accessioned 2020-05-13T11:09:58Z
dc.date.available 2020-05-13T11:09:58Z
dc.date.issued 2020-03-31
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/143069
dc.description.abstract [EN] Rex rabbit, with multiple phenotypes and colourful fur, is an interesting model for assessing the effect of coat colour gene mutations on characteristic pigmentation phenotype. Based on previous study, the melanophilin (MLPH) gene is a positional candidate gene related coat colour dilution. The fur colours are a lighter shade, e.g. grey instead of black. We sequenced 1689 base pairs of the MLPH gene in Chinchilla and black Rex rabbit. A total of 13 polymorphisms were identified, including seven missense mutations. The rabbit MLPH gene has a very high GC content and the protein shows 64.87% identity to the orthologous human protein (lack of homologous amino acids encoded by human MLPH exon 9). Hardy-Weinberg test showed that, except for the g.606C>A single nucleotid polymorphism (SNP), all other SNPs were in Hardy-Weinberg equilibrium. Haplotype analysis revealed that the seven missense mutation SNPs of two strains of Rex rabbits formed 10 haplotypes, but there were only seven major types of haplotypes (haplotype frequency P>0.05). The major haplotypes of the Chinchilla and black Rex rabbits were H1/H2/H3/H4/H5 and H1/H2/H3/H6/H8, respectively. The special haplotypes of Chinchilla Rex rabbit (H4, H5, H7) were consistently associated with the Chinchilla phenotype. This study provides evidence that different coat colour formation may be caused by one or more mutations within MLPH gene in several Rex rabbit strains. The data on polymorphisms that are associated with the Chinchilla phenotype facilitate the breeding of rabbits with defined coat colours. es_ES
dc.description.sponsorship This research was supported by the Modern Agricultural Industrial System Special Funding (CARS-43-A-1), the Science and Technology Major Project of New Variety Breeding (Livestock and Poultry) of Zhejiang Province, China (2016C02054-10), and National Natural Science Foundation of China (National Natural Science Foundation of China - Grant No. 31702081). es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.relation.ispartof World Rabbit Science es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject MLPH es_ES
dc.subject Rex rabbit es_ES
dc.subject Polymorphism es_ES
dc.subject Coat colour es_ES
dc.title Association of Melanophilin (MLPH) gene polymorphism with coat colour in Rex rabbits es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.4995/wrs.2020.12082
dc.relation.projectID info:eu-repo/grantAgreement/Earmarked Fund for Modern Agro-industry Technology Research System//CARS-43-A-1/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSFC//31702081/ es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Li, J.; Chen, Y.; Liu, M.; Chen, Q.; Zhou, J.; Bao, G.; Wu, X. (2020). Association of Melanophilin (MLPH) gene polymorphism with coat colour in Rex rabbits. World Rabbit Science. 28(1):29-38. https://doi.org/10.4995/wrs.2020.12082 es_ES
dc.description.accrualMethod OJS es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2020.12082 es_ES
dc.description.upvformatpinicio 29 es_ES
dc.description.upvformatpfin 38 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 28 es_ES
dc.description.issue 1 es_ES
dc.identifier.eissn 1989-8886
dc.relation.pasarela OJS\12082 es_ES
dc.contributor.funder Earmarked Fund for Modern Agro-industry Technology Research System, China es_ES
dc.contributor.funder National Natural Science Foundation of China es_ES
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