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Comparison between Tris-buffer and INRA-82 extenders on the quality of chilled rabbit spermatozoa

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Comparison between Tris-buffer and INRA-82 extenders on the quality of chilled rabbit spermatozoa

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dc.contributor.author Fadl, A.M. es_ES
dc.contributor.author Ghallab, A.M. es_ES
dc.contributor.author Abou-Ahmed, M.M. es_ES
dc.coverage.spatial east=174.885971; north=-40.90055699999999; name=Nova Zelanda es_ES
dc.date.accessioned 2020-05-13T11:12:23Z
dc.date.available 2020-05-13T11:12:23Z
dc.date.issued 2020-03-31
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/143070
dc.description.abstract [EN] The aim of the present study was to compare the effects of tris-buffer and INRA-82 extenders on the quality of cooled rabbit spermatozoa. Pooled semen samples were collected from eight New Zealand White rabbit bucks, divided into three groups and diluted 1:5 with three different semen extenders: INRA-82, tris-citrate glucose (TCG) and tris-citrate trehalose (TCT). Following dilution, the samples were stored at 5°C for up to 48 h. Sperm motility was recorded at 24, 36 and 48 h post-cooling. Furthermore, sperm viability, morphology, membrane integrity, acrosome status and DNA integrity were assessed at 24 h post-cooling. We found that progressive motility percentages were significantly higher (P<0.05) in samples diluted in INRA-82 (61.00, 53.50, 44.00% at 24, 36 and 48 h post-cooling, respectively) than those diluted in either TCG (39.25, 32.00, 19.75%) or in TCT (47.25, 40.50, 29.00%). We also reported that sperm viability, percentage of normal spermatozoa, percentage of spermatozoa with intact acrosome and DNA integrity after 24 h cooling were significantly higher (P<0.05) in INRA-82 diluted samples compared to TCG and TCT diluted ones. In summary, our results show that dilution of rabbit semen in INRA-82 improved sperm physiological parameters post-cooling compared to those diluted in TCG or TCT. Our findings also suggest that INRA-82 is a promising diluent that can be used effectively to maintain the viability of chilled rabbit semen. es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.relation.ispartof World Rabbit Science es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Rabbit bucks es_ES
dc.subject Dilution es_ES
dc.subject Cooling es_ES
dc.subject INRA82 es_ES
dc.subject TCT es_ES
dc.subject TCG es_ES
dc.title Comparison between Tris-buffer and INRA-82 extenders on the quality of chilled rabbit spermatozoa es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.4995/wrs.2020.12287
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Fadl, A.; Ghallab, A.; Abou-Ahmed, M. (2020). Comparison between Tris-buffer and INRA-82 extenders on the quality of chilled rabbit spermatozoa. World Rabbit Science. 28(1):13-18. https://doi.org/10.4995/wrs.2020.12287 es_ES
dc.description.accrualMethod OJS es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2020.12287 es_ES
dc.description.upvformatpinicio 13 es_ES
dc.description.upvformatpfin 18 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 28 es_ES
dc.description.issue 1 es_ES
dc.identifier.eissn 1989-8886
dc.relation.pasarela OJS\12287 es_ES
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