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dc.contributor.author | Roque Mesa, Edelin Marta | es_ES |
dc.contributor.author | Gómez Mena, María Concepción | es_ES |
dc.contributor.author | Hamza, Rim | es_ES |
dc.contributor.author | BELTRAN PORTER, JOSE PIO | es_ES |
dc.contributor.author | Cañas Clemente, Luís Antonio | es_ES |
dc.date.accessioned | 2020-05-19T03:02:28Z | |
dc.date.available | 2020-05-19T03:02:28Z | |
dc.date.issued | 2019-06-25 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/143617 | |
dc.description.abstract | [EN] Genetic engineered male sterility has different applications, ranging from hybrid seed production to bioconfinement of transgenes in genetic modified crops. The impact of this technology is currently patent in a wide range of crops, including legumes, which has helped to deal with the challenges of global food security. Production of engineered male sterile plants by expression of a ribonuclease gene under the control of an anther- or pollen-specific promoter has proven to be an efficient way to generate pollen-free elite cultivars. In the last years, we have been studying the genetic control of flower development in legumes and several genes that are specifically expressed in a determinate floral organ were identified. Pisum sativum ENDOTHECIUM 1 (PsEND1) is a pea anther-specific gene displaying very early expression in the anther primordium cells. This expression pattern has been assessed in both model plants and crops (tomato, tobacco, oilseed rape, rice, wheat) using genetic constructs carrying the PsEND1 promoter fused to the uidA reporter gene. This promoter fused to the barnase gene produces full anther ablation at early developmental stages, preventing the production of mature pollen grains in all plant species tested. Additional effects produced by the early anther ablation in the PsEND1::barnase-barstar plants, with interesting biotechnological applications, have also been described, such as redirection of resources to increase vegetative growth, reduction of the need for deadheading to extend the flowering period, or elimination of pollen allergens in ornamental plants (Kalanchoe, Pelargonium). Moreover, early anther ablation in transgenic PsEND1::barnase-barstar tomato plants promotes the developing of the ovaries into parthenocarpic fruits due to the absence of signals generated during the fertilization process and can be considered an efficient tool to promote fruit set and to produce seedless fruits. In legumes, the production of new hybrid cultivars will contribute to enhance yield and productivity by exploiting the hybrid vigor generated. The PsEND1::barnase-barstar construct could be also useful to generate parental lines in hybrid breeding approaches to produce new cultivars in different legume species. | es_ES |
dc.description.sponsorship | This work was funded by grants BIO2000-0940, BIO2000-0940, BIO2003-01171, BIO2006-09374, PTR95-0979-OP-03-01, RYC-2007-00627, AGL2009-13388-C03-01, AGL2009-07617, BIO2009-08134, AGL2015-64991-C3-3-R, and BIO2016-75485-R from the Spanish Ministry of Economy and Competitiveness (MINECO). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Frontiers Media SA | es_ES |
dc.relation.ispartof | Frontiers in Plant Science | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Bamase | es_ES |
dc.subject | Hybrid seeds | es_ES |
dc.subject | Male sterility | es_ES |
dc.subject | Parthenocarpy | es_ES |
dc.subject | Pisum sativum | es_ES |
dc.subject | Pollen allergens | es_ES |
dc.subject | PsEND1 promoter | es_ES |
dc.subject | Transgene bioconfinement | es_ES |
dc.title | Engineered Male Sterility by Early Anther Ablation Using the Pea Anther-Specific Promoter PsEND1 | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3389/fpls.2019.00819 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICYT//BIO2000-0940/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//PTR95-0979-OP-03-01/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//RYC-2007-00627/ES/RYC-2007-00627/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//AGL2009-13388-C03-01/ES/Generacion De Mutantes De Insercion De Tomate Cultivado Y Silvestre E Identificacion De Genes Implicados En Procesos Del Desarrollo Y Tolerancia A Estres Abiotico/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//AGL2015-64991-C3-3-R/ES/GENOMICA FUNCIONAL Y MEJORA GENETICA DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO - ESTRES ABIOTICO/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICYT//BIO2003-01171/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//BIO2006-09374/ES/ANALISIS GENETICO Y FUNCIONAL DEL DESARROLLO FLORAL EN MEDICAGO TRUNCATULA/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BIO2009-08134/ES/Mejora Del Valor Nutritivo De La Alfalfa (Medicago Sativa L.) Mediante Ingenieria Genetica/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//AGL2009-07617/ES/Analisis Genetico De La Androesterilidad Como Herramienta Para La Mejora Del Cuajado Y La Calidad Del Fruto De Variedades Comerciales De Tomate./ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BIO2016-75485-R/ES/CONTROL GENETICO DEL DESARROLLO FLORAL EN LAS LEGUMINOSAS: PAPEL DE LOS GENES CATASTRALES EN LA FORMACION DE LOS ORGANOS REPRODUCTIVOS/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Roque Mesa, EM.; Gómez Mena, MC.; Hamza, R.; Beltran Porter, JP.; Cañas Clemente, LA. (2019). Engineered Male Sterility by Early Anther Ablation Using the Pea Anther-Specific Promoter PsEND1. Frontiers in Plant Science. 10:1-9. https://doi.org/10.3389/fpls.2019.00819 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3389/fpls.2019.00819 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 9 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 10 | es_ES |
dc.identifier.eissn | 1664-462X | es_ES |
dc.identifier.pmid | 31293612 | es_ES |
dc.identifier.pmcid | PMC6603094 | es_ES |
dc.relation.pasarela | S\406615 | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación | |
dc.contributor.funder | Ministerio de Ciencia y Tecnología | es_ES |
dc.contributor.funder | Ministerio de Educación y Ciencia | es_ES |
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