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Single Primer Enrichment Technology (SPET) for High-Throughput Genotyping in Tomato and Eggplant Germplasm

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Single Primer Enrichment Technology (SPET) for High-Throughput Genotyping in Tomato and Eggplant Germplasm

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dc.contributor.author Barchi, L. es_ES
dc.contributor.author Acquadro, A. es_ES
dc.contributor.author Alonso-Martín, David es_ES
dc.contributor.author Aprea, G. es_ES
dc.contributor.author Bassolino, L. es_ES
dc.contributor.author Demurtas, O.C. es_ES
dc.contributor.author Ferrante, P. es_ES
dc.contributor.author Gramazio, Pietro es_ES
dc.contributor.author Mini, P. es_ES
dc.contributor.author Portis, Ezio es_ES
dc.contributor.author Scaglione, D. es_ES
dc.contributor.author Toppino, L. es_ES
dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.contributor.author Díez Niclós, Mª José Teresa De Jesús es_ES
dc.contributor.author Rotino, G.L. es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.date.accessioned 2020-05-20T03:01:42Z
dc.date.available 2020-05-20T03:01:42Z
dc.date.issued 2019-08-07 es_ES
dc.identifier.uri http://hdl.handle.net/10251/143786
dc.description.abstract [EN] Single primer enrichment technology (SPET) is a new, robust, and customizable solution for targeted genotyping. Unlike genotyping by sequencing (GBS), and like DNA chips, SPET is a targeted genotyping technology, relying on the sequencing of a region flanking a primer. Its reliance on single primers, rather than on primer pairs, greatly simplifies panel design, and allows higher levels of multiplexing than PCR-based genotyping. Thanks to the sequencing of the regions surrounding the target SNP, SPET allows the discovery of thousands of closely linked, novel SNPs. In order to assess the potential of SPET for high-throughput genotyping in plants, a panel comprising 5k target SNPs, designed both on coding regions and introns/UTRs, was developed for tomato and eggplant. Genotyping of two panels composed of 400 tomato and 422 eggplant accessions, comprising both domesticated material and wild relatives, generated a total of 12,002 and 30,731 high confidence SNPs, respectively, which comprised both target and novel SNPs in an approximate ratio of 1:1.6, and 1:5.5 in tomato and eggplant, respectively. The vast majority of the markers was transferrable to related species that diverged up to 3.4 million years ago (Solanum pennellii for tomato and S. macrocarpon for eggplant). Maximum Likelihood phylogenetic trees and PCA outputs obtained from the whole dataset highlighted genetic relationships among accessions and species which were congruent with what was previously reported in literature. Better discrimination among domesticated accessions was achieved by using the target SNPs, while better discrimination among wild species was achieved using the whole SNP dataset. Our results reveal that SPET genotyping is a robust, high-throughput technology for genetic fingerprinting, with a high degree of cross-transferability between crops and their cultivated and wild relatives, and allows identification of duplicates and mislabeled accessions in genebanks. es_ES
dc.description.sponsorship This work has been funded by the European Union's Horizon 2020 Research and Innovation Programme under the grant agreement number 677379 (G2P-SOL project: Linking genetic resources, genomes, and phenotypes of solanaceous crops). es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Plant Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject SPET es_ES
dc.subject Genotyping es_ES
dc.subject Tomato es_ES
dc.subject Eggplant es_ES
dc.subject Germplasm es_ES
dc.subject.classification GENETICA es_ES
dc.title Single Primer Enrichment Technology (SPET) for High-Throughput Genotyping in Tomato and Eggplant Germplasm es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fpls.2019.01005 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/677379/EU/Linking genetic resources, genomes and phenotypes of Solanaceous crops/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Barchi, L.; Acquadro, A.; Alonso-Martín, D.; Aprea, G.; Bassolino, L.; Demurtas, O.; Ferrante, P.... (2019). Single Primer Enrichment Technology (SPET) for High-Throughput Genotyping in Tomato and Eggplant Germplasm. Frontiers in Plant Science. 10:1-17. https://doi.org/10.3389/fpls.2019.01005 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fpls.2019.01005 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 17 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.identifier.eissn 1664-462X es_ES
dc.identifier.pmid 31440267 es_ES
dc.identifier.pmcid PMC6693525 es_ES
dc.relation.pasarela S\407111 es_ES
dc.description.references Acquadro, A., Barchi, L., Gramazio, P., Portis, E., Vilanova, S., Comino, C., … Lanteri, S. (2017). Coding SNPs analysis highlights genetic relationships and evolution pattern in eggplant complexes. PLOS ONE, 12(7), e0180774. doi:10.1371/journal.pone.0180774 es_ES
dc.description.references Anderson, J. A., Churchill, G. A., Autrique, J. E., Tanksley, S. D., & Sorrells, M. E. (1993). Optimizing parental selection for genetic linkage maps. Genome, 36(1), 181-186. doi:10.1139/g93-024 es_ES
dc.description.references Barchi, L., Pietrella, M., Venturini, L., Minio, A., Toppino, L., Acquadro, A., … Rotino, G. L. (2019). A chromosome-anchored eggplant genome sequence reveals key events in Solanaceae evolution. Scientific Reports, 9(1). doi:10.1038/s41598-019-47985-w es_ES
dc.description.references Beddows, I., Reddy, A., Kloesges, T., & Rose, L. E. (2017). Population Genomics in Wild Tomatoes—The Interplay of Divergence and Admixture. Genome Biology and Evolution, 9(11), 3023-3038. doi:10.1093/gbe/evx224 es_ES
dc.description.references Blanca, J., Montero-Pau, J., Sauvage, C., Bauchet, G., Illa, E., Díez, M. J., … Cañizares, J. (2015). Genomic variation in tomato, from wild ancestors to contemporary breeding accessions. BMC Genomics, 16(1). doi:10.1186/s12864-015-1444-1 es_ES
dc.description.references Caicedo, A. L., & Schaal, B. A. (2004). Population structure and phylogeography of Solanum pimpinellifolium inferred from a nuclear gene. Molecular Ecology, 13(7), 1871-1882. doi:10.1111/j.1365-294x.2004.02191.x es_ES
dc.description.references Castle, J. C. (2011). SNPs Occur in Regions with Less Genomic Sequence Conservation. PLoS ONE, 6(6), e20660. doi:10.1371/journal.pone.0020660 es_ES
dc.description.references Cericola, F., Portis, E., Toppino, L., Barchi, L., Acciarri, N., Ciriaci, T., … Lanteri, S. (2013). The Population Structure and Diversity of Eggplant from Asia and the Mediterranean Basin. PLoS ONE, 8(9), e73702. doi:10.1371/journal.pone.0073702 es_ES
dc.description.references Chen, K.-Y., Cong, B., Wing, R., Vrebalov, J., & Tanksley, S. D. (2007). Changes in Regulation of a Transcription Factor Lead to Autogamy in Cultivated Tomatoes. Science, 318(5850), 643-645. doi:10.1126/science.1148428 es_ES
dc.description.references Chen, K.-Y., & Tanksley, S. D. (2004). High-Resolution Mapping and Functional Analysis ofse2.1. Genetics, 168(3), 1563-1573. doi:10.1534/genetics.103.022558 es_ES
dc.description.references Danecek, P., Auton, A., Abecasis, G., Albers, C. A., Banks, E., … DePristo, M. A. (2011). The variant call format and VCFtools. Bioinformatics, 27(15), 2156-2158. doi:10.1093/bioinformatics/btr330 es_ES
dc.description.references Davey, J. W., Hohenlohe, P. A., Etter, P. D., Boone, J. Q., Catchen, J. M., & Blaxter, M. L. (2011). Genome-wide genetic marker discovery and genotyping using next-generation sequencing. Nature Reviews Genetics, 12(7), 499-510. doi:10.1038/nrg3012 es_ES
dc.description.references Del Fabbro, C., Scalabrin, S., Morgante, M., & Giorgi, F. M. (2013). An Extensive Evaluation of Read Trimming Effects on Illumina NGS Data Analysis. PLoS ONE, 8(12), e85024. doi:10.1371/journal.pone.0085024 es_ES
dc.description.references DePristo, M. A., Banks, E., Poplin, R., Garimella, K. V., Maguire, J. R., Hartl, C., … Daly, M. J. (2011). A framework for variation discovery and genotyping using next-generation DNA sequencing data. Nature Genetics, 43(5), 491-498. doi:10.1038/ng.806 es_ES
dc.description.references Dodsworth, S., Chase, M. W., Särkinen, T., Knapp, S., & Leitch, A. R. (2015). Using genomic repeats for phylogenomics: a case study in wild tomatoes (SolanumsectionLycopersicon: Solanaceae). Biological Journal of the Linnean Society, 117(1), 96-105. doi:10.1111/bij.12612 es_ES
dc.description.references Elshire, R. J., Glaubitz, J. C., Sun, Q., Poland, J. A., Kawamoto, K., Buckler, E. S., & Mitchell, S. E. (2011). A Robust, Simple Genotyping-by-Sequencing (GBS) Approach for High Diversity Species. PLoS ONE, 6(5), e19379. doi:10.1371/journal.pone.0019379 es_ES
dc.description.references Flint-Garcia, S. A. (2013). Genetics and Consequences of Crop Domestication. Journal of Agricultural and Food Chemistry, 61(35), 8267-8276. doi:10.1021/jf305511d es_ES
dc.description.references Gramazio, P., Prohens, J., Borràs, D., Plazas, M., Herraiz, F. J., & Vilanova, S. (2017). Comparison of transcriptome-derived simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers for genetic fingerprinting, diversity evaluation, and establishment of relationships in eggplants. Euphytica, 213(12). doi:10.1007/s10681-017-2057-3 es_ES
dc.description.references Hoang, D. T., Chernomor, O., von Haeseler, A., Minh, B. Q., & Vinh, L. S. (2017). UFBoot2: Improving the Ultrafast Bootstrap Approximation. Molecular Biology and Evolution, 35(2), 518-522. doi:10.1093/molbev/msx281 es_ES
dc.description.references Hoheisel, J. D. (2006). Microarray technology: beyond transcript profiling and genotype analysis. Nature Reviews Genetics, 7(3), 200-210. doi:10.1038/nrg1809 es_ES
dc.description.references Huerta-Cepas, J., Serra, F., & Bork, P. (2016). ETE 3: Reconstruction, Analysis, and Visualization of Phylogenomic Data. Molecular Biology and Evolution, 33(6), 1635-1638. doi:10.1093/molbev/msw046 es_ES
dc.description.references Isshiki, S., Iwata, N., & Khan, M. M. R. (2008). ISSR variations in eggplant (Solanum melongena L.) and related Solanum species. Scientia Horticulturae, 117(3), 186-190. doi:10.1016/j.scienta.2008.04.003 es_ES
dc.description.references Kamenetzky, L., Asís, R., Bassi, S., de Godoy, F., Bermúdez, L., Fernie, A. R., … Carrari, F. (2010). Genomic Analysis of Wild Tomato Introgressions Determining Metabolism- and Yield-Associated Traits. Plant Physiology, 152(4), 1772-1786. doi:10.1104/pp.109.150532 es_ES
dc.description.references Kouassi, B., Prohens, J., Gramazio, P., Kouassi, A. B., Vilanova, S., Galán-Ávila, A., … Plazas, M. (2016). Development of backcross generations and new interspecific hybrid combinations for introgression breeding in eggplant ( Solanum melongena ). Scientia Horticulturae, 213, 199-207. doi:10.1016/j.scienta.2016.10.039 es_ES
dc.description.references Li, H., Handsaker, B., Wysoker, A., Fennell, T., Ruan, J., … Homer, N. (2009). The Sequence Alignment/Map format and SAMtools. Bioinformatics, 25(16), 2078-2079. doi:10.1093/bioinformatics/btp352 es_ES
dc.description.references Lin, T., Zhu, G., Zhang, J., Xu, X., Yu, Q., Zheng, Z., … Huang, S. (2014). Genomic analyses provide insights into the history of tomato breeding. Nature Genetics, 46(11), 1220-1226. doi:10.1038/ng.3117 es_ES
dc.description.references Lynch, V. J., & Wagner, G. P. (2010). DID EGG-LAYING BOAS BREAK DOLLO’S LAW? PHYLOGENETIC EVIDENCE FOR REVERSAL TO OVIPARITY IN SAND BOAS (ERYX: BOIDAE). Evolution, 64(1), 207-216. doi:10.1111/j.1558-5646.2009.00790.x es_ES
dc.description.references Mammadov, J., Aggarwal, R., Buyyarapu, R., & Kumpatla, S. (2012). SNP Markers and Their Impact on Plant Breeding. International Journal of Plant Genomics, 2012, 1-11. doi:10.1155/2012/728398 es_ES
dc.description.references Martin, M. (2011). Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet.journal, 17(1), 10. doi:10.14806/ej.17.1.200 es_ES
dc.description.references Mason, A. S., Zhang, J., Tollenaere, R., Vasquez Teuber, P., Dalton-Morgan, J., Hu, L., … Batley, J. (2015). High-throughput genotyping for species identification and diversity assessment in germplasm collections. Molecular Ecology Resources, 15(5), 1091-1101. doi:10.1111/1755-0998.12379 es_ES
dc.description.references Meyer, R. S., Karol, K. G., Little, D. P., Nee, M. H., & Litt, A. (2012). Phylogeographic relationships among Asian eggplants and new perspectives on eggplant domestication. Molecular Phylogenetics and Evolution, 63(3), 685-701. doi:10.1016/j.ympev.2012.02.006 es_ES
dc.description.references Miz, R. B., Mentz, L. A., & Souza-Chies, T. T. (2007). Overview of the phylogenetic relationships of some southern Brazilian species from section Torva and related sections of «spiny Solanum» (Solanum subgenus Leptostemonum, Solanaceae). Genetica, 132(2), 143-158. doi:10.1007/s10709-007-9156-3 es_ES
dc.description.references Nairismägi, M.-L., Tan, J., Lim, J. Q., Nagarajan, S., Ng, C. C. Y., Rajasegaran, V., … Ong, C. K. (2016). JAK-STAT and G-protein-coupled receptor signaling pathways are frequently altered in epitheliotropic intestinal T-cell lymphoma. Leukemia, 30(6), 1311-1319. doi:10.1038/leu.2016.13 es_ES
dc.description.references Nguyen, L.-T., Schmidt, H. A., von Haeseler, A., & Minh, B. Q. (2014). IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies. Molecular Biology and Evolution, 32(1), 268-274. doi:10.1093/molbev/msu300 es_ES
dc.description.references Pailles, Y., Ho, S., Pires, I. S., Tester, M., Negrão, S., & Schmöckel, S. M. (2017). Genetic Diversity and Population Structure of Two Tomato Species from the Galapagos Islands. Frontiers in Plant Science, 8. doi:10.3389/fpls.2017.00138 es_ES
dc.description.references Herraiz, F. J., Blanca, J., Ziarsolo, P., Gramazio, P., Plazas, M., Anderson, G. J., … Vilanova, S. (2016). The first de novo transcriptome of pepino (Solanum muricatum): assembly, comprehensive analysis and comparison with the closely related species S. caripense, potato and tomato. BMC Genomics, 17(1). doi:10.1186/s12864-016-2656-8 es_ES
dc.description.references Plazas, M., Andújar, I., Vilanova, S., Gramazio, P., Herraiz, F. J., & Prohens, J. (2014). Conventional and phenomics characterization provides insight into the diversity and relationships of hypervariable scarlet (Solanum aethiopicum L.) and gboma (S. macrocarpon L.) eggplant complexes. Frontiers in Plant Science, 5. doi:10.3389/fpls.2014.00318 es_ES
dc.description.references Plazas, M., Vilanova, S., Gramazio, P., Rodríguez-Burruezo, A., Fita, A., Herraiz, F. J., … Prohens, J. (2016). Interspecific Hybridization between Eggplant and Wild Relatives from Different Genepools. Journal of the American Society for Horticultural Science, 141(1), 34-44. doi:10.21273/jashs.141.1.34 es_ES
dc.description.references Poplin, R., Ruano-Rubio, V., DePristo, M. A., Fennell, T. J., Carneiro, M. O., Van der Auwera, G. A., … Banks, E. (2017). Scaling accurate genetic variant discovery to tens of thousands of samples. doi:10.1101/201178 es_ES
dc.description.references Razali, R., Bougouffa, S., Morton, M. J. L., Lightfoot, D. J., Alam, I., Essack, M., … Negrão, S. (2018). The Genome Sequence of the Wild Tomato Solanum pimpinellifolium Provides Insights Into Salinity Tolerance. Frontiers in Plant Science, 9. doi:10.3389/fpls.2018.01402 es_ES
dc.description.references Robinson, D. F., & Foulds, L. R. (1981). Comparison of phylogenetic trees. Mathematical Biosciences, 53(1-2), 131-147. doi:10.1016/0025-5564(81)90043-2 es_ES
dc.description.references Rodriguez, F., Wu, F., Ané, C., Tanksley, S., & Spooner, D. M. (2009). Do potatoes and tomatoes have a single evolutionary history, and what proportion of the genome supports this history? BMC Evolutionary Biology, 9(1), 191. doi:10.1186/1471-2148-9-191 es_ES
dc.description.references Sakata, Y., & Lester, R. N. (1997). Euphytica, 97(3), 295-301. doi:10.1023/a:1003000612441 es_ES
dc.description.references Sakata, Y., Nishio, T., & Matthews, P. J. (1991). Chloroplast DNA analysis of eggplant (Solanum melongena) and related species for their taxonomic affinity. Euphytica, 55(1), 21-26. doi:10.1007/bf00022555 es_ES
dc.description.references Särkinen, T., Bohs, L., Olmstead, R. G., & Knapp, S. (2013). A phylogenetic framework for evolutionary study of the nightshades (Solanaceae): a dated 1000-tip tree. BMC Evolutionary Biology, 13(1), 214. doi:10.1186/1471-2148-13-214 es_ES
dc.description.references Scaglione, D., Pinosio, S., Marroni, F., Di Centa, E., Fornasiero, A., Magris, G., … Morgante, M. (2019). Single primer enrichment technology as a tool for massive genotyping: a benchmark on black poplar and maize. Annals of Botany, 124(4), 543-551. doi:10.1093/aob/mcz054 es_ES
dc.description.references Scheben, A., Batley, J., & Edwards, D. (2017). Genotyping-by-sequencing approaches to characterize crop genomes: choosing the right tool for the right application. Plant Biotechnology Journal, 15(2), 149-161. doi:10.1111/pbi.12645 es_ES
dc.description.references Scolnick, J. A., Dimon, M., Wang, I.-C., Huelga, S. C., & Amorese, D. A. (2015). An Efficient Method for Identifying Gene Fusions by Targeted RNA Sequencing from Fresh Frozen and FFPE Samples. PLOS ONE, 10(7), e0128916. doi:10.1371/journal.pone.0128916 es_ES
dc.description.references Semagn, K., Babu, R., Hearne, S., & Olsen, M. (2013). Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): overview of the technology and its application in crop improvement. Molecular Breeding, 33(1), 1-14. doi:10.1007/s11032-013-9917-x es_ES
dc.description.references Sim, S.-C., Van Deynze, A., Stoffel, K., Douches, D. S., Zarka, D., Ganal, M. W., … Francis, D. M. (2012). High-Density SNP Genotyping of Tomato (Solanum lycopersicum L.) Reveals Patterns of Genetic Variation Due to Breeding. PLoS ONE, 7(9), e45520. doi:10.1371/journal.pone.0045520 es_ES
dc.description.references Syfert, M. M., Castañeda-Álvarez, N. P., Khoury, C. K., Särkinen, T., Sosa, C. C., Achicanoy, H. A., … Knapp, S. (2016). Crop wild relatives of the brinjal eggplant (Solanum melongena): Poorly represented in genebanks and many species at risk of extinction. American Journal of Botany, 103(4), 635-651. doi:10.3732/ajb.1500539 es_ES
dc.description.references (2012). The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 485(7400), 635-641. doi:10.1038/nature11119 es_ES
dc.description.references Thomson, R. C., & Shaffer, H. B. (2010). Sparse Supermatrices for Phylogenetic Inference: Taxonomy, Alignment, Rogue Taxa, and the Phylogeny of Living Turtles. Systematic Biology, 59(1), 42-58. doi:10.1093/sysbio/syp075 es_ES
dc.description.references Tranchida-Lombardo, V., Mercati, F., Avino, M., Punzo, P., Fiore, M. C., Poma, I., … Grillo, S. (2018). Genetic diversity in a collection of Italian long storage tomato landraces as revealed by SNP markers array. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 153(2), 288-297. doi:10.1080/11263504.2018.1478900 es_ES
dc.description.references Vilanova, S., Manzur, J. P., & Prohens, J. (2011). Development and characterization of genomic simple sequence repeat markers in eggplant and their application to the study of diversity and relationships in a collection of different cultivar types and origins. Molecular Breeding, 30(2), 647-660. doi:10.1007/s11032-011-9650-2 es_ES
dc.description.references Vorontsova, M. S., Stern, S., Bohs, L., & Knapp, S. (2013). African spinySolanum(subgenusLeptostemonum, Solanaceae): a thorny phylogenetic tangle. Botanical Journal of the Linnean Society, 173(2), 176-193. doi:10.1111/boj.12053 es_ES
dc.description.references Weese, T. L., & Bohs, L. (2010). Eggplant origins: Out of Africa, into the Orient. TAXON, 59(1), 49-56. doi:10.1002/tax.591006 es_ES
dc.description.references Tan, M. H., Gan, H. M., Schultz, M. B., & Austin, C. M. (2015). MitoPhAST, a new automated mitogenomic phylogeny tool in the post-genomic era with a case study of 89 decapod mitogenomes including eight new freshwater crayfish mitogenomes. Molecular Phylogenetics and Evolution, 85, 180-188. doi:10.1016/j.ympev.2015.02.009 es_ES
dc.description.references Wiens, J. J., & Morrill, M. C. (2011). Missing Data in Phylogenetic Analysis: Reconciling Results from Simulations and Empirical Data. Systematic Biology, 60(5), 719-731. doi:10.1093/sysbio/syr025 es_ES
dc.description.references Williams, C. E., & Clair, D. A. S. (1993). Phenetic relationships and levels of variability detected by restriction fragment length polymorphism and random amplified polymorphic DNA analysis of cultivated and wild accessions of Lycopersicon esculentum. Genome, 36(3), 619-630. doi:10.1139/g93-083 es_ES
dc.description.references Zheng, X., Levine, D., Shen, J., Gogarten, S. M., Laurie, C., & Weir, B. S. (2012). A high-performance computing toolset for relatedness and principal component analysis of SNP data. Bioinformatics, 28(24), 3326-3328. doi:10.1093/bioinformatics/bts606 es_ES


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