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Colorimetric detection of normetanephrine, a pheochromocytoma biomarker, using bifunctionalised gold nanoparticles

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Colorimetric detection of normetanephrine, a pheochromocytoma biomarker, using bifunctionalised gold nanoparticles

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dc.contributor.author Godoy-Reyes, Tania Mariel es_ES
dc.contributor.author Costero, Ana M. es_ES
dc.contributor.author Gaviña, Pablo es_ES
dc.contributor.author Martínez-Máñez, Ramón es_ES
dc.contributor.author Sancenón Galarza, Félix es_ES
dc.date.accessioned 2020-06-02T05:36:49Z
dc.date.available 2020-06-02T05:36:49Z
dc.date.issued 2019-05-16 es_ES
dc.identifier.issn 0003-2670 es_ES
dc.identifier.uri http://hdl.handle.net/10251/144805
dc.description.abstract [EN] A simple and effective colorimetric method for the detection of normetanephrine (NMN), an O-methylated metabolite of norepinephrine, using functionalised gold nanoparticles is described. This metabolite is an important biomarker in the diagnosis of adrenal tumours such as pheocromocytoma or paraganglioma. The colorimetric probe consists of spherical gold nanoparticles (AuNPs) functionalised with two different ligands, which specifically recognize different functional groups in normetanephrine. Thus, a benzaldehyde-terminated ligand was used for the recognition of the amino alcohol moiety in NMN, by forming the corresponding oxazolidine. On the other hand, N-acetyl-cysteine was chosen for the recognition of the phenolic hydroxyl group through the formation of hydrogen bonds. The selective double molecular recognition between the probe and the hydroxyl and the amino-alcohol moieties of normetanephrine led to interparticle-crosslinking aggregation resulting in a change in the color of the solution, from red to blue, which could be observed by naked eye. The probe was highly selective towards normetanephrine and no color changes were observed in the presence of other neurotransmitter metabolites such as homovanillic acid (HVA) (dopamine metabolite), 5-hydroxyindoleacetic acid (5-HIAA) (serotonin metabolite), or other biomolecules present in urine such as glucose (Glc), uric acid (U.A), and urea. Finally, the probe was evaluated in synthetic urine with constituents that mimic human urine, where a limit of detection of 0.5 mu M was achieved. es_ES
dc.description.sponsorship Financial support from the Spanish Government (project MAT2015-64139-C4) and Generalitat Valenciana (Project PROMETEOII/2014/047 and AICO/2017/093) is gratefully acknowledged. T. Godoy-Reyes is grateful to Generalitat Valenciana for her Santiago Grisolia fellowship. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation Generalitat Valenciana/AICO/2017/093 es_ES
dc.relation GENERALITAT VALENCIANA es_ES
dc.relation GENERALITAT VALENCIANA/PROMETEOII/2014/047 es_ES
dc.relation MINISTERIO DE ECONOMIA Y EMPRESA/MAT2015-64139-C4-1-R es_ES
dc.relation.ispartof Analytica Chimica Acta es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Normetanephrine es_ES
dc.subject Gold nanoparticles es_ES
dc.subject Colorimetric detection es_ES
dc.subject Aggregation es_ES
dc.subject Pheochromocytoma es_ES
dc.subject.classification QUIMICA INORGANICA es_ES
dc.subject.classification QUIMICA ORGANICA es_ES
dc.title Colorimetric detection of normetanephrine, a pheochromocytoma biomarker, using bifunctionalised gold nanoparticles es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.aca.2019.01.003 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Godoy-Reyes, TM.; Costero, AM.; Gaviña, P.; Martínez-Máñez, R.; Sancenón Galarza, F. (2019). Colorimetric detection of normetanephrine, a pheochromocytoma biomarker, using bifunctionalised gold nanoparticles. Analytica Chimica Acta. 1056:146-152. https://doi.org/10.1016/j.aca.2019.01.003 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.aca.2019.01.003 es_ES
dc.description.upvformatpinicio 146 es_ES
dc.description.upvformatpfin 152 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 1056 es_ES
dc.identifier.pmid 30797455 es_ES
dc.relation.pasarela S\377316 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Economía y Empresa es_ES


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