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The role of a class III gibberellin 2-oxidase in tomato internode elongation

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The role of a class III gibberellin 2-oxidase in tomato internode elongation

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dc.contributor.author Lavelle, A. es_ES
dc.contributor.author Gath, N. es_ES
dc.contributor.author Devisetty, U. es_ES
dc.contributor.author Carrera Bergua, Esther es_ES
dc.contributor.author Lopez Diaz, Isabel es_ES
dc.contributor.author Blazquez Rodriguez, Miguel Angel es_ES
dc.contributor.author Maloof, J. es_ES
dc.date.accessioned 2020-06-19T03:30:29Z
dc.date.available 2020-06-19T03:30:29Z
dc.date.issued 2018-11-05 es_ES
dc.identifier.issn 0960-7412 es_ES
dc.identifier.uri http://hdl.handle.net/10251/146635
dc.description.abstract [EN] A network of environmental inputs and internal signaling controls plant growth, development and organ elongation. In particular, the growth-promoting hormone gibberellin (GA) has been shown to play a significant role in organ elongation. The use of tomato as a model organism to study elongation presents an opportunity to study the genetic control of internode-specific elongation in a eudicot species with a sympodial growth habit and substantial internodes that can and do respond to external stimuli. To investigate internode elongation, a mutant with an elongated hypocotyl and internodes but wild-type petioles was identified through a forward genetic screen. In addition to stem-specific elongation, this mutant, named tomato internode elongated -1 (tie-1) is more sensitive to the GA biosynthetic inhibitor paclobutrazol and has altered levels of intermediate and bioactive GAs compared with wild-type plants. The mutation responsible for the internode elongation phenotype was mapped to GA2oxidase 7, a class III GA 2-oxidase in the GA biosynthetic pathway, through a bulked segregant analysis and bioinformatic pipeline, and confirmed by transgenic complementation. Furthermore, bacterially expressed recombinant TIE protein was shown to have bona fide GA 2-oxidase activity. These results define a critical role for this gene in internode elongation and are significant because they further the understanding of the role of GA biosynthetic genes in organ-specific elongation. es_ES
dc.description.sponsorship This work used the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley, supported by NIH S10 Instrumentation Grants S10RR029668 and S10RR027303. We thank the Tomato Genetics Resource Center for providing seed of the M82 and Heinz cultivars. The material was developed by and/or obtained from the UC Davis/C M Rick Tomato Genetics Resource Center and maintained by the Department of Plant Sciences, University of California, Davis, CA 95616, USA. We thank Anthony Bolger, Alisdair Fernie and Bjorn Usadel for providing us with access to pre-publication genomic reads of the S. lycopersicum cultivar M82, and Cristina Urbez and Noel Blanco-Tourinan (IBMCP, Spain) for technical help with in vitro production of TIE1. This work was supported in part by the Elsie Taylor Stocking Memorial Fellowship awarded to ASL in 2013, by NSF grant IOS-0820854, by USDA National Institute of Food and Agriculture project CA-D-PLB-2465-H, by internal UC Davis funds, and by Spanish Ministry of Economy and Competitiveness grant BFU2016-80621-P. es_ES
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation.ispartof The Plant Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Gibberellins es_ES
dc.subject Class III GA 2-oxidase es_ES
dc.subject Solanum lycopersicum es_ES
dc.subject Tomato es_ES
dc.subject Internode elongation es_ES
dc.subject Bulked segregant analysis es_ES
dc.title The role of a class III gibberellin 2-oxidase in tomato internode elongation es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/tpj.14145 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//S10RR027303/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSF//0820854/US/Elucidating Gene Networks Regulating Development in Tomato/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//S10RR029668/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIFA//CA-D-PLB-2465-H/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2016-80621-P/ES/ANÁLISIS EVOLUTIVO DE UN 'HUB' FUNCIONAL EN PLANTAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Lavelle, A.; Gath, N.; Devisetty, U.; Carrera Bergua, E.; Lopez Diaz, I.; Blazquez Rodriguez, MA.; Maloof, J. (2018). The role of a class III gibberellin 2-oxidase in tomato internode elongation. The Plant Journal. https://doi.org/10.1111/tpj.14145 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1111/tpj.14145 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.relation.pasarela S\382291 es_ES
dc.contributor.funder University of California, Davis es_ES
dc.contributor.funder National Science Foundation, EEUU es_ES
dc.contributor.funder National Institutes of Health, EEUU es_ES
dc.contributor.funder National Institute of Food and Agriculture, EEUU es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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