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Design and validation of a bio-mechanical bioreactor for cartilage tissue culture

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Design and validation of a bio-mechanical bioreactor for cartilage tissue culture

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dc.contributor.author Correia, V. es_ES
dc.contributor.author Panadero, J. A. es_ES
dc.contributor.author Ribeiro, C. es_ES
dc.contributor.author Sencadas, V. es_ES
dc.contributor.author Rocha, J. G. es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.contributor.author Lanceros-Méndez, S. es_ES
dc.date.accessioned 2020-06-23T03:46:43Z
dc.date.available 2020-06-23T03:46:43Z
dc.date.issued 2016-04 es_ES
dc.identifier.issn 1617-7959 es_ES
dc.identifier.uri http://hdl.handle.net/10251/146775
dc.description.abstract [EN] Specific tissues, such as cartilage, undergo mechanical solicitation under their normal performance in human body. In this sense, it seems necessary that proper tissue engineering strategies of these tissues should incorporate mechanical solicitations during cell culture, in order to properly evaluate the influence of the mechanical stimulus. This work reports on a user-friendly bioreactor suitable for applying controlled mechanical stimulation¿amplitude and frequency¿to three-dimensional scaffolds. Its design and main components are described, as well as its operation characteristics. The modular design allows easy cleaning and operating under laminar hood. Different protocols for the sterilization of the hermetic enclosure are tested and ensure lack of observable contaminations, complying with the requirements to be used for cell culture. The cell viability study was performed with KUM5 cells. es_ES
dc.description.sponsorship This work was funded by FEDER funds through the Programa Operacional Fatores de Competitividade COMPETE and by national funds arranged by FCT Fundação para a Ciência e a Tecnologia, project reference PEST-C/FIS/UI607/2014. The authors also thank funding from Matepro Optimizing Materials and Processes , ref. NORTE-07-0124-FEDER-000037 , co-funded by the Programa Operacional Regional do Norte (ON.2 O Novo Norte), under the Quadro de Referência Estratégico Nacional (QREN), through the Fundo Europeu de Desenvolvimento Regional (FEDER). JAP, VS, CR and VC thank the FCT for the SFRH/BD/64586/2009 and SFRH/BPD/63148/2009, SFRH/BPD/90870/2012, and SFRH/BPD/ 97739/2013 grants, respectively. This work was funded also by the Spanish Ministry of Economy and Copetitiveness (MINECO) through the project MAT2013-46467-C4-1-R (including the FEDER financial support). CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008 2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Biomechanics and Modeling in Mechanobiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Bioreactor es_ES
dc.subject Mechanical stimulation es_ES
dc.subject Scaffold es_ES
dc.subject Cell culture es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Design and validation of a bio-mechanical bioreactor for cartilage tissue culture es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10237-015-0698-5 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2013-46467-C4-1-R/ES/ESTIMULACION MECANICA LOCAL DE CELULAS MESENQUIMALES DE CARA A SU DIFERENCIACION OSTEOGENICA Y CONDROGENICA EN MEDICINA REGENERATIVA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F63148%2F2009/PT/ELECTROACTIVE MATERIALS BASED POROUS MEMBRANES AND SCAFFOLDS FOR BIOMEDICAL APPLICATIONS/
dc.relation.projectID info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F97739%2F2013/PT/DEVELOPMENT OF A PIEZORESISITIVE SENSOR MATRIX TO ASSESS THE FORCES ON THE LOWER LIMB SOCKET\STUMP INTERFACE/
dc.relation.projectID info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F64586%2F2009/PT/ACTIVE BIOMATERIALS FOR CELL CULTURE UNDER MECHANICAL STIMULUS APPLIED TO CARTILAGE TISSUE ENGINEERING/
dc.relation.projectID info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F90870%2F2012/PT/TAILORING ELECTRO-MECHANICALLY ACTIVE MATERIALS FOR TISSUE ENGINEERING APPLICATIONS/
dc.relation.projectID info:eu-repo/grantAgreement/FCT/PEST-C/FIS%2FUI607%2F2014/PT/
dc.relation.projectID info:eu-repo/grantAgreement/FEDER//FEDER/NORTE-07-0124-FEDER-000037/2013/EU/Matepro Optimizing Materials and Processes/
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Correia, V.; Panadero, JA.; Ribeiro, C.; Sencadas, V.; Rocha, JG.; Gómez Ribelles, JL.; Lanceros-Méndez, S. (2016). Design and validation of a bio-mechanical bioreactor for cartilage tissue culture. Biomechanics and Modeling in Mechanobiology. 15(2):471-478. https://doi.org/10.1007/s10237-015-0698-5 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s10237-015-0698-5 es_ES
dc.description.upvformatpinicio 471 es_ES
dc.description.upvformatpfin 478 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 15 es_ES
dc.description.issue 2 es_ES
dc.relation.pasarela S\325491 es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES
dc.contributor.funder Fundação para a Ciência e a Tecnologia, Portugal es_ES
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