González Martínez, MÁ.; Morais, S.; Puchades, R.; Maquieira Catala, A.; Marco, M.; Barceló, D. (1998). Automation of Heterogeneous Enzyme Immunoassay for Atrazine. Comparison of Three Immobilization Supports. Fresenius Journal of Analytical Chemistry. 361(2):179-184. https://doi.org/10.1007/s002160050858
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/146777
Title:
|
Automation of Heterogeneous Enzyme Immunoassay for Atrazine. Comparison of Three Immobilization Supports
|
Author:
|
González Martínez, Miguel Ángel
Morais, Sergi
Puchades, Rosa
Maquieira Catala, Angel
Marco, M.
Barceló, D.
|
UPV Unit:
|
Universitat Politècnica de València. Departamento de Química - Departament de Química
|
Issued date:
|
|
Abstract:
|
[EN] Intensive agriculture associated with the use of large amounts of different pesticides, together with the growing concern about the potential contamination of ground water, have brought about the need for developing ...[+]
[EN] Intensive agriculture associated with the use of large amounts of different pesticides, together with the growing concern about the potential contamination of ground water, have brought about the need for developing fast screening methods. This work presents the automation of an enzyme-linked immunosorbent assay for atrazine by means of a flow-through system. Three different solid supports for antibody immobilization were compared in a direct competitive assay format. Sensitivity reached in all cases was below the maximum level allowed in the EU (100 ng L-1). Cross-reactivity of atrazine-related compounds was also studied. The performance of the different supports is discussed regarding sensitivity and immunosurface regeneration.
[-]
|
Copyrigths:
|
Cerrado |
Source:
|
Fresenius Journal of Analytical Chemistry. (issn:
0937-0633
)
|
DOI:
|
10.1007/s002160050858
|
Publisher:
|
Springer-Verlag
|
Publisher version:
|
https://doi.org/ 10.1007/s002160050858
|
Project ID:
|
CICYT/AMB 96-1079
EC/ENV4-CT96-0333
MINISTERIO DE EDUCACION /PB95-0740
|
Thanks:
|
This work was supported by the CICYT project AMB 96-1079 and the DGCYT project PB 95-0740, Comission of the European Community (Contract ENV4-CT96-0333). M.A.González-Martínez acknowledges a grant from the Fundación ...[+]
This work was supported by the CICYT project AMB 96-1079 and the DGCYT project PB 95-0740, Comission of the European Community (Contract ENV4-CT96-0333). M.A.González-Martínez acknowledges a grant from the Fundación Cajamadrid, Spain, to carry out a PhD degree.
[-]
|
Type:
|
Artículo
|