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Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A as an Intermediate Layer

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Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A as an Intermediate Layer

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dc.contributor.author Caroselli, Raffaele es_ES
dc.contributor.author Garcia Castello, Javier es_ES
dc.contributor.author Escorihuela Fuentes, Jorge es_ES
dc.contributor.author Bañuls Polo, María-José es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.contributor.author García-Rupérez, Jaime es_ES
dc.date.accessioned 2020-07-02T06:50:17Z
dc.date.available 2020-07-02T06:50:17Z
dc.date.issued 2018-03-28 es_ES
dc.identifier.uri http://hdl.handle.net/10251/147306
dc.description.abstract [EN] A proper antibody immobilization on a biosensor is a crucial step in order to obtain a high sensitivity to be able to detect low target analyte concentrations. In this paper, we present an experimental study of the immobilization process of antibodies as bioreceptors on a photonic ring resonator sensor. A protein A intermediate layer was created on the sensor surface in order to obtain an oriented immobilization of the antibodies, which enhances the interaction with the target antigens to be detected. The anti-bovine serum albumin (antiBSA)-bovine serum albumin (BSA) pair was used as a model for our study. An opto-fluidic setup was developed in order to flow the different reagents and, simultaneously, to monitor in real-time the spectral response of the photonic sensing structure. The antiBSA immobilization and the BSA detection, their repeatability, and specificity were studied in different conditions of the sensor surface. Finally, an experimental limit of detection for BSA recognition of only 1 ng/mL was obtained es_ES
dc.description.sponsorship Funding from Spanish government through grants TEC2015-63838-C3-1-R (MINECO/FEDER, UE), TEC2013-49987-EXP BIOGATE, and CTQ/2016/75749-R, and from the European Commission through project H2020-644242-SAPHELY is acknowledged. Raffaele Caroselli also acknowledges the Generalitat Valenciana for funding his grant through the doctoral scholarship GRISOLIAP/2014/109. es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation GV/GRISOLIA/2014/109 es_ES
dc.relation MINISTERIO DE ECONOMIA INDUSTRIA Y COMPETITIVIDAD /TEC2013-49987-EXP es_ES
dc.relation MINISTERIO DE ECONOMIA INDUSTRIA Y COMPETITIVIDAD /TEC2015-63838-C3-1-R es_ES
dc.relation AEI/CTQ2016-75749-R es_ES
dc.relation.ispartof Sensors es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Integrated photonics es_ES
dc.subject Ring resonator es_ES
dc.subject Sensor es_ES
dc.subject Biosensing es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.subject.classification TEORIA DE LA SEÑAL Y COMUNICACIONES es_ES
dc.title Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A as an Intermediate Layer es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/s18041012 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/644242/EU/Self-amplified photonic biosensing platform for microRNA-based early diagnosis of diseases/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Comunicaciones - Departament de Comunicacions es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Tecnología Nanofotónica - Institut Universitari de Tecnologia Nanofotònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Caroselli, R.; Garcia Castello, J.; Escorihuela Fuentes, J.; Bañuls Polo, M.; Maquieira Catala, Á.; García-Rupérez, J. (2018). Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A as an Intermediate Layer. Sensors. 18(4):1-15. https://doi.org/10.3390/s18041012 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/s18041012 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 15 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 18 es_ES
dc.description.issue 4 es_ES
dc.identifier.eissn 1424-8220 es_ES
dc.identifier.pmid 29597326 es_ES
dc.identifier.pmcid PMC5949038 es_ES
dc.relation.pasarela S\356711 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES


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