Mostrar el registro sencillo del ítem
dc.contributor.author | Caroselli, Raffaele | es_ES |
dc.contributor.author | Garcia Castello, Javier | es_ES |
dc.contributor.author | Escorihuela Fuentes, Jorge | es_ES |
dc.contributor.author | Bañuls Polo, María-José | es_ES |
dc.contributor.author | Maquieira Catala, Ángel | es_ES |
dc.contributor.author | García-Rupérez, Jaime | es_ES |
dc.date.accessioned | 2020-07-02T06:50:17Z | |
dc.date.available | 2020-07-02T06:50:17Z | |
dc.date.issued | 2018-03-28 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/147306 | |
dc.description.abstract | [EN] A proper antibody immobilization on a biosensor is a crucial step in order to obtain a high sensitivity to be able to detect low target analyte concentrations. In this paper, we present an experimental study of the immobilization process of antibodies as bioreceptors on a photonic ring resonator sensor. A protein A intermediate layer was created on the sensor surface in order to obtain an oriented immobilization of the antibodies, which enhances the interaction with the target antigens to be detected. The anti-bovine serum albumin (antiBSA)-bovine serum albumin (BSA) pair was used as a model for our study. An opto-fluidic setup was developed in order to flow the different reagents and, simultaneously, to monitor in real-time the spectral response of the photonic sensing structure. The antiBSA immobilization and the BSA detection, their repeatability, and specificity were studied in different conditions of the sensor surface. Finally, an experimental limit of detection for BSA recognition of only 1 ng/mL was obtained | es_ES |
dc.description.sponsorship | Funding from Spanish government through grants TEC2015-63838-C3-1-R (MINECO/FEDER, UE), TEC2013-49987-EXP BIOGATE, and CTQ/2016/75749-R, and from the European Commission through project H2020-644242-SAPHELY is acknowledged. Raffaele Caroselli also acknowledges the Generalitat Valenciana for funding his grant through the doctoral scholarship GRISOLIAP/2014/109. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.relation.ispartof | Sensors | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Integrated photonics | es_ES |
dc.subject | Ring resonator | es_ES |
dc.subject | Sensor | es_ES |
dc.subject | Biosensing | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.subject.classification | TEORIA DE LA SEÑAL Y COMUNICACIONES | es_ES |
dc.title | Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A as an Intermediate Layer | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/s18041012 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/644242/EU/Self-amplified photonic biosensing platform for microRNA-based early diagnosis of diseases/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//GRISOLIA%2F2014%2F109/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//TEC2013-49987-EXP/ES/BIOSENSORES NANOFOTONICOS ULTRASENSIBLES BASADOS EN REACCION EN CADENA MEDIANTE PUERTAS MOLECULARES NANOSCOPICAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//TEC2015-63838-C3-1-R/ES/DETECCION DE TOXINAS Y AGENTES PATOGENOS MEDIANTE BIOSENSORES OPTICOS NANOMETRICOS PARA AMENAZAS NBQ/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CTQ2016-75749-R/ES/BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICAS/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Comunicaciones - Departament de Comunicacions | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario de Tecnología Nanofotónica - Institut Universitari de Tecnologia Nanofotònica | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada | es_ES |
dc.description.bibliographicCitation | Caroselli, R.; Garcia Castello, J.; Escorihuela Fuentes, J.; Bañuls Polo, M.; Maquieira Catala, Á.; García-Rupérez, J. (2018). Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A as an Intermediate Layer. Sensors. 18(4):1-15. https://doi.org/10.3390/s18041012 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/s18041012 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 15 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 18 | es_ES |
dc.description.issue | 4 | es_ES |
dc.identifier.eissn | 1424-8220 | es_ES |
dc.identifier.pmid | 29597326 | es_ES |
dc.identifier.pmcid | PMC5949038 | es_ES |
dc.relation.pasarela | S\356711 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |