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alpha 2-COP is involved in early secretory traffic in Arabidopsis and is required for plant growth

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alpha 2-COP is involved in early secretory traffic in Arabidopsis and is required for plant growth

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dc.contributor.author Gimeno-Ferrer, Fatima es_ES
dc.contributor.author Pastor-Cantizano, Noelia es_ES
dc.contributor.author Bernat-Silvestre, Cesar es_ES
dc.contributor.author Selvi-Martinez, Pilar es_ES
dc.contributor.author Vera Sirera, Francisco José es_ES
dc.contributor.author Gao, Caiji es_ES
dc.contributor.author PEREZ AMADOR, MIGUEL ANGEL es_ES
dc.contributor.author Jiang, Liwen es_ES
dc.contributor.author Aniento, Fernando es_ES
dc.contributor.author Marcote, Maria Jesús es_ES
dc.date.accessioned 2020-07-02T06:50:36Z
dc.date.available 2020-07-02T06:50:36Z
dc.date.issued 2017-01 es_ES
dc.identifier.issn 0022-0957 es_ES
dc.identifier.uri http://hdl.handle.net/10251/147312
dc.description.abstract [EN] COP (coat protein) I-coated vesicles mediate intra-Golgi transport and retrograde transport from the Golgi to the endoplasmic reticulum. These vesicles form through the action of the small GTPase ADP-ribosylation factor 1 (ARF1) and the COPI heptameric protein complex (coatomer), which consists of seven subunits (alpha-, beta-, beta' -, gamma-, delta-, epsilon- and xi-COP). In contrast to mammals and yeast, several isoforms for coatomer subunits, with the exception of gamma and delta, have been identified in Arabidopsis. To understand the role of COPI proteins in plant biology, we have identified and characterized a loss-of-function mutant of alpha 2-COP, an Arabidopsis alpha-COP isoform. The alpha 2-cop mutant displayed defects in plant growth, including small rosettes, stems and roots and mislocalization of p24 delta 5, a protein of the p24 family containing a C-terminal dilysine motif involved in COPI binding. The alpha 2-cop mutant also exhibited abnormal morphology of the Golgi apparatus. Global expression analysis of the alpha 2-cop mutant revealed altered expression of plant cell wall-associated genes. In addition, a strong upregulation of SEC31A, which encodes a subunit of the COPII coat, was observed in the alpha 2-cop mutant; this also occurs in a mutant of a gene upstream of COPI assembly, GNL1, which encodes an ARF-guanine nucleotide exchange factor (GEF). These findings suggest that loss of alpha 2-COP affects the expression of secretory pathway genes. es_ES
dc.description.sponsorship Antibody against alpha-COP was kindly provided by Prof. F. Wieland. This work was supported by the Ministerio de Economia y Competitividad (grant number. BFU2012-33883 to FA and MJM; grant numbers BIO2011-26302 and BIO2014-55946-P to MP), the Generalitat Valenciana (grant numbers ISIC/2013/004 and GVACOMP2014-202 to FA and MJM) and the Research Grants Council (grant number AoE/M-05/12 to LJ). NP and CB were recipients of a fellowship from Ministerio de Educacion (FPU program). We thank the Salk Institute Genomic Analysis Laboratory for providing the sequence-indexed Arabidopsis T-DNA insertion mutants and the microscopy and genomics section of SCSIE (University of Valencia). es_ES
dc.language Inglés es_ES
dc.publisher Oxford University Press es_ES
dc.relation.ispartof Journal of Experimental Botany es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Alpha 1-COP es_ES
dc.subject Alpha 2-COP es_ES
dc.subject Arabidopsis es_ES
dc.subject Coat protein es_ES
dc.subject COPI es_ES
dc.subject COPII, Golgi apparatus es_ES
dc.subject P24 family protein es_ES
dc.subject SEC31 es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title alpha 2-COP is involved in early secretory traffic in Arabidopsis and is required for plant growth es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/jxb/erw446 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2011-26302/ES/ANALISIS DE LOS MECANISMOS MOLECULARES DE LA SEÑALIZACION POR GIBERELINAS EN LA FRUCTIFICACION DE ARABIDOPSIS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2012-33883/ES/TRAFICO INTRACELULAR DE PROTEINAS EN CELULAS VEGETALES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ISIC%2F2013%2F004/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACOMP%2F2014%2F202/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/RGC//AoE%2FM-05%2F12/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2014-55946-P/ES/LAS GIBERELINAS EN EL CONTROL DE LA MORFOGENESIS DE LOS OVULOS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Gimeno-Ferrer, F.; Pastor-Cantizano, N.; Bernat-Silvestre, C.; Selvi-Martinez, P.; Vera Sirera, FJ.; Gao, C.; Perez Amador, MA.... (2017). alpha 2-COP is involved in early secretory traffic in Arabidopsis and is required for plant growth. Journal of Experimental Botany. 68(3):391-401. https://doi.org/10.1093/jxb/erw446 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1093/jxb/erw446 es_ES
dc.description.upvformatpinicio 391 es_ES
dc.description.upvformatpfin 401 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 68 es_ES
dc.description.issue 3 es_ES
dc.identifier.pmid 28025315 es_ES
dc.identifier.pmcid PMC5441910 es_ES
dc.relation.pasarela S\356555 es_ES
dc.contributor.funder Ministerio de Ciencia, Innovación y Universidades es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Deutsche Forschungsgemeinschaft es_ES
dc.contributor.funder Research Grant Council, Hong Kong es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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