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dc.contributor.author | Cordero-Cucart, María Teresa | es_ES |
dc.contributor.author | Rosado, A. | es_ES |
dc.contributor.author | Majer, Eszter | es_ES |
dc.contributor.author | Jaramillo Rosales, Alfonso | es_ES |
dc.contributor.author | Rodrigo Tarrega, Guillermo | es_ES |
dc.contributor.author | Daros Arnau, Jose Antonio | es_ES |
dc.date.accessioned | 2020-07-02T06:50:57Z | |
dc.date.available | 2020-07-02T06:50:57Z | |
dc.date.issued | 2018-10 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/147319 | |
dc.description.abstract | [EN] Due to autotrophic growing capacity and extremely rich secondary metabolism, plants should be preferred targets of synthetic biology. However, developments in plants usually run below those in other taxonomic groups. In this work we engineered genetic circuits capable of logic YES, OR and AND Boolean computation in plant tissues with a visual output signal. The circuits, which are deployed by means of Agrobacterium tumefaciens, perform with the conditional activity of the MYB transcription factor Roseal from Antirrhinum majus inducing the accumulation of anthocyanins, plant endogenous pigments that are directly visible to the naked eye or accurately quantifiable by spectrophotometric analysis. The translational fusion of Roseal to several viral proteins, such as potyvirus NIb or fragments thereof, rendered the transcription factor inactive. However, anthocyanin accumulation could be restored by inserting protease cleavage sites between both moieties of the fusion and by coexpressing specific proteases, such as potyvirus nuclear inclusion a protease. | es_ES |
dc.description.sponsorship | This research was supported by the Ministerio de Ciencia, Innovacion y Universidades (Spain) grants AGL2013-49919-EXP, BIO2014-54269-R, BFU2015-66894-P and BIO2017-83184-R (cofinanced FEDER funds) and by the Engineering and Physical Sciences Research Council and the Biotechnology and Biological Sciences Research Council (UK) grant BB/M017982/1. E.M. was the recipient of a predoctoral fellowship (AP2012-3751) from the Ministerio de Educacion, Cultura y Deporte (Spain). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.relation.ispartof | ACS Synthetic Biology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Anthocyanins, Biological computing | es_ES |
dc.subject | Genetic circuits | es_ES |
dc.subject | Potyvirus protease | es_ES |
dc.subject | Synthetic biology | es_ES |
dc.subject.classification | GENETICA | es_ES |
dc.title | Boolean Computation in Plants Using Post-translational Genetic Control and a Visual Output Signal | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1021/acssynbio.8b00214 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UKRI//BB%2FM017982%2F1/GB/Warwick Integrative Synthetic Biology Centre/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MECD//AP2012-3751/ES/AP2012-3751/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//AGL2013-49919-EXP/ES/DETECCION DE PATOGENOS Y BIOCOMPUTACION MEDIANTE CIRCUITOS REGULADORES EN PLANTAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BIO2014-54269-R/ES/INSTRUMENTOS BIOTECNOLOGICOS DERIVADOS DE VIRUS DE PLANTAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83184-R/ES/VIRUS DE PLANTAS: PATOGENOS Y TAMBIEN VECTORES PARA LA PRODUCCION DE PROTEINAS, METABOLITOS, RNAS Y NANOPARTICULAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BFU2015-66894-P /ES/MODELADO, DISEÑO DE NOVO E INGENIERIA DE INTERRUPTORES DE RNA QUE RESPONDEN A SEÑALES GENETICAS/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.description.bibliographicCitation | Cordero-Cucart, MT.; Rosado, A.; Majer, E.; Jaramillo Rosales, A.; Rodrigo Tarrega, G.; Daros Arnau, JA. (2018). Boolean Computation in Plants Using Post-translational Genetic Control and a Visual Output Signal. ACS Synthetic Biology. 7(10):2322-2330. https://doi.org/10.1021/acssynbio.8b00214 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1021/acssynbio.8b00214 | es_ES |
dc.description.upvformatpinicio | 2322 | es_ES |
dc.description.upvformatpfin | 2330 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 7 | es_ES |
dc.description.issue | 10 | es_ES |
dc.identifier.eissn | 2161-5063 | es_ES |
dc.identifier.pmid | 30212620 | es_ES |
dc.relation.pasarela | S\382302 | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | UK Research and Innovation | es_ES |
dc.contributor.funder | Ministerio de Educación, Cultura y Deporte | es_ES |
dc.contributor.funder | Engineering and Physical Sciences Research Council, Reino Unido | es_ES |
dc.contributor.funder | Biotechnology and Biological Sciences Research Council, Reino Unido | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |