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Detecting Nonvolatile Life- and Nonlife-Derived Organics in a Carbonaceous Chondrite Analogue with a New Multiplex Immunoassay and Its Relevance for Planetary Exploration

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Detecting Nonvolatile Life- and Nonlife-Derived Organics in a Carbonaceous Chondrite Analogue with a New Multiplex Immunoassay and Its Relevance for Planetary Exploration

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dc.contributor.author Moreno-Paz, Mercedes es_ES
dc.contributor.author Gómez-Cifuentes, Ana es_ES
dc.contributor.author Ruiz-Bermejo, Marta es_ES
dc.contributor.author Hofstetter, Oliver es_ES
dc.contributor.author Maquieira Catala, Angel es_ES
dc.contributor.author Manchado, Juan M. es_ES
dc.contributor.author Morais, Sergi es_ES
dc.contributor.author Sephton, Mark A. es_ES
dc.contributor.author Niessner, Reinhard es_ES
dc.contributor.author Knopp, Dietmar es_ES
dc.contributor.author Parro, Victor es_ES
dc.date.accessioned 2020-07-07T03:32:53Z
dc.date.available 2020-07-07T03:32:53Z
dc.date.issued 2018-08-01 es_ES
dc.identifier.issn 1531-1074 es_ES
dc.identifier.uri http://hdl.handle.net/10251/147527
dc.description.abstract [EN] Potential martian molecular targets include those supplied by meteoritic carbonaceous chondrites such as amino acids and polycyclic aromatic hydrocarbons and true biomarkers stemming from any hypothetical martian biota (organic architectures that can be directly related to once living organisms). Heat extraction and pyrolysis-based methods currently used in planetary exploration are highly aggressive and very often modify the target molecules making their identification a cumbersome task. We have developed and validated a mild, nondestructive, multiplex inhibitory microarray immunoassay and demonstrated its implementation in the SOLID (Signs of Life Detector) instrument for simultaneous detection of several nonvolatile life- and nonlife-derived organic molecules relevant in planetary exploration and environmental monitoring. By utilizing a set of highly specific antibodies that recognize D- or L- aromatic amino acids (Phe, Tyr, Trp), benzo[a]pyrene (B[a]P), pentachlorophenol, and sulfone-containing aromatic compounds, respectively, the assay was validated in the SOLID instrument for the analysis of carbon-rich samples used as analogues of the organic material in carbonaceous chondrites or even Mars samples. Most of the antibodies enabled sensitivities at the 1-10ppb level and some even at the ppt level. The multiplex immunoassay allowed the detection of B[a]P as well as aromatic sulfones in a water/methanol extract of an Early Cretaceous lignite sample (c.a., 140 Ma) representing type IV kerogen. No L- or D-aromatic amino acids were detected, reflecting the advanced diagenetic stage and the fossil nature of the sample. The results demonstrate the ability of the liquid extraction by ultrasonication and the versatility of the multiplex inhibitory immunoassays in the SOLID instrument to discriminate between organic matter derived from life and nonlife processes, an essential step toward life detection outside Earth. es_ES
dc.description.sponsorship This work was supported by granted projects AYA2011-24803, ESP2014-51989-P, and ESP2015-69540-R, from the Ministry of Economy and Competitiveness (MINECO) of Spain, and by Grant No. ST/N000803/1 (United Kingdom). A. G-C was a fellow of Plan de Formación from INTA. es_ES
dc.language Inglés es_ES
dc.publisher Mary Ann Liebert es_ES
dc.relation.ispartof Astrobiology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Planetary exploration es_ES
dc.subject Molecular biomarkers es_ES
dc.subject D- and L- aromatic amino acids es_ES
dc.subject Life detection es_ES
dc.subject Multiplex inhibitory/competitive immunoassay es_ES
dc.subject Kerogen type IV es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Detecting Nonvolatile Life- and Nonlife-Derived Organics in a Carbonaceous Chondrite Analogue with a New Multiplex Immunoassay and Its Relevance for Planetary Exploration es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1089/ast.2017.1747 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AYA2011-24803/ES/DETECCION DE BIOMOLECULAS EN EXPLORACION PLANETARIA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//ST%2FN000803%2F1/GB/Planetary Origins and Evolution at Imperial (2016-2019)/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//ESP2014-51989-P/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//ESP2015-69540-R/ES/DETECCION DE BIOMOLECULAS EN EXPLORACION PLANETARIA/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Moreno-Paz, M.; Gómez-Cifuentes, A.; Ruiz-Bermejo, M.; Hofstetter, O.; Maquieira Catala, A.; Manchado, JM.; Morais, S.... (2018). Detecting Nonvolatile Life- and Nonlife-Derived Organics in a Carbonaceous Chondrite Analogue with a New Multiplex Immunoassay and Its Relevance for Planetary Exploration. Astrobiology. 18(8):1041-1056. https://doi.org/10.1089/ast.2017.1747 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1089/ast.2017.1747 es_ES
dc.description.upvformatpinicio 1041 es_ES
dc.description.upvformatpfin 1056 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 18 es_ES
dc.description.issue 8 es_ES
dc.identifier.pmid 29638146 es_ES
dc.identifier.pmcid PMC6225596 es_ES
dc.relation.pasarela S\361100 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder UK Research and Innovation es_ES
dc.contributor.funder Science and Technology Facilities Council, Reino Unido es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES


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