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The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways

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The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways

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dc.contributor.author Gomez-Muñoz, Neus es_ES
dc.contributor.author Velazquez, K. es_ES
dc.contributor.author Vives, M.C. es_ES
dc.contributor.author Ruiz-Ruiz, Susana es_ES
dc.contributor.author Pina, J.A. es_ES
dc.contributor.author FLORES PEDAUYE, RICARDO es_ES
dc.contributor.author Moreno, P. es_ES
dc.contributor.author Guerri, J. es_ES
dc.date.accessioned 2020-07-23T03:31:07Z
dc.date.available 2020-07-23T03:31:07Z
dc.date.issued 2017-12 es_ES
dc.identifier.issn 1464-6722 es_ES
dc.identifier.uri http://hdl.handle.net/10251/148517
dc.description.abstract [EN] Citrus tristeza virus (CTV) induces in the field the decline and death of citrus varieties grafted on sour orange (SO) rootstock, which has forced the use of alternative decline-tolerant rootstocks in affected countries, despite the highly desirable agronomic features of the SO rootstock. Declining citrus plants display phloem necrosis below the bud union. In addition, SO is minimally susceptible to CTV compared with other citrus varieties, suggesting partial resistance of SO to CTV. Here, by silencing different citrus genes with a Citrus leaf blotch virus-based vector, we have examined the implication of the RNA silencing and salicylic acid (SA) defence pathways in the resistance of SO to CTV. Silencing of the genes RDR1, NPR1 and DCL2/DCL4, associated with these defence pathways, enhanced virus spread and accumulation in SO plants in comparison with non-silenced controls, whereas silencing of the genes NPR3/NPR4, associated with the hypersensitive response, produced a slight decrease in CTV accumulation and reduced stunting of SO grafted on CTV-infected rough lemon plants. We also found that the CTV RNA silencing suppressors p20 and p23 also suppress the SA signalling defence, with the suppressor activity being higher in the most virulent isolates. es_ES
dc.description.sponsorship This work was supported by grant AGL2012-32429, co-financed by ERDF (European Regional Development Fund) and the Spanish Ministerio de Economia y Competitividad (MINECO). We thank Dr W. O. Dawson for providing the GFP-tagged T36 isolate of CTV. Neus Gomez was the recipient of a doctoral fellowship from MINECO. Maria-Carmen Vives was the recipient of a contract from Instituto Valenciano de Investigaciones Agrarias (IVIA). es_ES
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation.ispartof Molecular Plant Pathology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Citrus decline es_ES
dc.subject CLBV es_ES
dc.subject Reverse genetics es_ES
dc.subject Salicylic acid signalling defence suppressor es_ES
dc.subject Viral vector es_ES
dc.title The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/mpp.12488 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2012-32429/ES/DESARROLLO DE METODOS AVANZADOS PARA LA MEJORA SANITARIA Y GENETICA DE LOS CITRICOS/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Gomez-Muñoz, N.; Velazquez, K.; Vives, M.; Ruiz-Ruiz, S.; Pina, J.; Flores Pedauye, R.; Moreno, P.... (2017). The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways. Molecular Plant Pathology. 18(9):1253-1266. https://doi.org/10.1111/mpp.12488 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1111/mpp.12488 es_ES
dc.description.upvformatpinicio 1253 es_ES
dc.description.upvformatpfin 1266 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 18 es_ES
dc.description.issue 9 es_ES
dc.identifier.pmid 27588892 es_ES
dc.identifier.pmcid PMC6638288 es_ES
dc.relation.pasarela S\357588 es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Institut Valencià d'Investigacions Agràries es_ES
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