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Molecular characterisation of antimicrobial resistance and virulence genes in Escherichia coli strains isolated from diarrhoeic and healthy rabbits in Tunisia

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Molecular characterisation of antimicrobial resistance and virulence genes in Escherichia coli strains isolated from diarrhoeic and healthy rabbits in Tunisia

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dc.contributor.author Ben Rhouma, Raouia es_ES
dc.contributor.author Jouini, Ahlem es_ES
dc.contributor.author Klibi, Amira es_ES
dc.contributor.author Hamrouni, Safa es_ES
dc.contributor.author Boubaker, Aziza es_ES
dc.contributor.author Kmiha, Souhir es_ES
dc.contributor.author Maaroufi, Abderrazek es_ES
dc.coverage.spatial east=9.537499; north=33.886917; name=Governació de Gabès, Tunísia es_ES
dc.date.accessioned 2020-07-27T07:12:17Z
dc.date.available 2020-07-27T07:12:17Z
dc.date.issued 2020-06-30
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/148721
dc.description.abstract [EN] The purpose of this study was to identify Escherichia coli isolates in diarrhoeic and healthy rabbits in Tunisia and characterise their virulence and antibiotic resistance genes. In the 2014-2015 period, 60 faecal samples from diarrhoeic and healthy rabbits were collected from different breeding farms in Tunisia. Susceptibility to 14 antimicrobial agents was tested by disc diffusion method and the mechanisms of gene resistance were evaluated using polymerase chain reaction and sequencing methods. Forty E. coli isolates were recovered in selective media. High frequency of resistance to tetracycline (95%) was detected, followed by different levels of resistance to sulphonamide (72.5%), streptomycin (62.5%), trimethoprim-sulfamethoxazole (60%), nalidixic acid (32.5%), ampicillin (37.5%) and ticarcillin (35%). E. coli strains were susceptible to cefotaxime, ceftazidime and imipenem. Different variants of blaTEM, tet, sul genes were detected in most of the strains resistant to ampicillin, tetracycline and sulphonamide, respectively. The presence of class 1 integron was studied in 29 sulphonamide-resistant E. coli strains from which 15 harboured class 1 integron with four different arrangements of gene cassettes, dfrA17+aadA5 (n=9), dfrA1 + aadA1 (n=4), dfrA12 + addA2 (n=1), dfrA12+orf+addA2 (n=1). The qnrB gene was detected in six strains out of 13 quinolone-resistant E. coli strains. Seventeen E. coli isolates from diarrhoeic rabbits harboured the enteropathogenic eae genes associated with different virulence genes tested (fimA, cnf1, aer), and affiliated to B2 (n=8) and D (n=9) phylogroups. Isolated E. coli strains from healthy rabbit were harbouring fim A and/or cnf1 genes and affiliated to A and B1 phylogroups. This study showed that E. coli strains from the intestinal tract of rabbits are resistant to the widely prescribed antibiotics in medicine. Therefore, they constitute a reservoir of antimicrobial-resistant genes, which may play a significant role in the spread of antimicrobial resistance. In addition, the eae virulence gene seemed to be implicated in diarrhoea in breeder rabbits in Tunisia. es_ES
dc.description.sponsorship The work was supported by Tunisian Ministry of Higher Education, Scientific Research and Technology (LR16IP03). Many thanks go to the members of the Department of Animal Production, National Institute of Agronomy of Tunisia for their help in collecting the samples. es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.relation.ispartof World Rabbit Science es_ES
dc.rights Reconocimiento - No comercial - Compartir igual (by-nc-sa) es_ES
dc.subject Antibiotic resistance genes es_ES
dc.subject Breeding rabbits es_ES
dc.subject E. coli isolates es_ES
dc.subject Integrons es_ES
dc.subject Virulence genes es_ES
dc.subject Tunisia es_ES
dc.title Molecular characterisation of antimicrobial resistance and virulence genes in Escherichia coli strains isolated from diarrhoeic and healthy rabbits in Tunisia es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.4995/wrs.2020.10879
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Ben Rhouma, R.; Jouini, A.; Klibi, A.; Hamrouni, S.; Boubaker, A.; Kmiha, S.; Maaroufi, A. (2020). Molecular characterisation of antimicrobial resistance and virulence genes in Escherichia coli strains isolated from diarrhoeic and healthy rabbits in Tunisia. World Rabbit Science. 28(2):81-91. https://doi.org/10.4995/wrs.2020.10879 es_ES
dc.description.accrualMethod OJS es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2020.10879 es_ES
dc.description.upvformatpinicio 81 es_ES
dc.description.upvformatpfin 91 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 28 es_ES
dc.description.issue 2 es_ES
dc.identifier.eissn 1989-8886
dc.relation.pasarela OJS\10879 es_ES
dc.contributor.funder Ministère de l'Enseignement Supérieur et de la Recherche Scientifique, Túnez es_ES
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